Restriction map for
pF1KE0573
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaBI BseAI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CfoI ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 445 GTCTAC GT^[AC][GT]AC
AocI 73 CCTGAGG CC^T[ACGT]AGG
BbsI 175 GAAGAC GAAGAC(2/6)
BglI 755 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 372 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 372 GCTGAGC GC^T[ACGT]AGC
BpuAI 175 GAAGAC GAAGAC(2/6)
BsaAI 448 TACGTG [CT]AC^GT[GA]
BsaWI 794 ACCGGA [AT]^CCGG[AT]
BsrBI 18 CCGCTC CCGCTC(-3/-3)
BsrDI 242 CATTGC GCAATG(2/0)
BsrFI 801 GCCGGC [GA]^CCGG[CT]
BstYI 439 AGATCC [GA]^GATC[CT]
Bsu36I 73 CCTGAGG CC^T[ACGT]AGG
CelII 372 GCTGAGC GC^T[ACGT]AGC
Cfr10I 801 GCCGGC [GA]^CCGG[CT]
Eco81I 73 CCTGAGG CC^T[ACGT]AGG
FauI 701 GCGGG CCCGC(4/6)
MflI 439 AGATCC [GA]^GATC[CT]
NaeI 801 GCCGGC GCC^GGC
NcoI 287 CCATGG C^CATGG
NgoMIV 801 GCCGGC G^CCGGC
NlaIII 288 CATG CATG^
PpuMI 406 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 367 CAGCTG CAG^CTG
SgrAI 800 CGCCGGCG C[GA]^CCGG[CT]G
StuI 44 AGGCCT AGG^CCT
TaqI 711 TCGA T^CGA
TfiI 114 GATTC G^A[AT]TC
TspRI 809 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 711 TCGA T^CGA
XcmI 107 CCAAGGAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 439 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 357 CGCG CG^CG
700 CGCG
AvaI 12 CCCGAG C^[CT]CG[GA]G
412 CCCGAG
BanI 352 GGCACC G^G[CT][GA]CC
735 GGCACC
BsgI 325 GTGCAG GTGCAG(16/14)
601 GTGCAG
BsiEI 359 CGGCCG CG[GA][CT]^CG
829 CGGCCG
BsmFI 304 GGGAC GGGAC(10/14)
406 GGGAC
BsoBI 12 CCCGAG C^[CT]CG[GA]G
412 CCCGAG
BstUI 357 CGCG CG^CG
700 CGCG
EaeI 359 CGGCCG [CT]^GGCC[GA]
829 CGGCCG
EagI 359 CGGCCG C^GGCCG
829 CGGCCG
EclXI 359 CGGCCG C^GGCCG
829 CGGCCG
Eco52I 359 CGGCCG C^GGCCG
829 CGGCCG
KspI 356 CCGCGG CCGC^GG
699 CCGCGG
MaeIII 526 GTCAC ^GT[ACGT]AC
539 GTGAC
MslI 267 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
648 CATCAAGGTG
MvnI 357 CGCG CG^CG
700 CGCG
MwoI 204 GCTGTCTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
755 GCCTCCAGGGC
PflMI 124 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
252 CCAGGAGCTGG
PleI 39 GACTC GAGTC(4/5)
524 GAGTC
SacII 356 CCGCGG CCGC^GG
699 CCGCGG
SfaNI 166 GATGC GCATC(5/9)
190 GATGC
Tsp45I 526 GTCAC ^GT[GC]AC
539 GTGAC
Van91I 124 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
252 CCAGGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
AvaII BanII BbvI BcnI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI Hin1I HinfI HpaII
ItaI Ksp632I MaeII MboI MboII
MnlI MspA1I MspI MvaI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
StyI TseI