Restriction map for
pF1KE0579
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbvCI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstYI
BstZ17I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FbaI FokI FseI FspI HgaI
HincII HindII HpaI KpnI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
MvnI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcsI 148 GAATTC [GA]^AATT[CT]
AcyI 62 GGCGCC G[GA]^CG[CT]C
AlwI 345 GATCC GGATC(4/5)
AlwNI 377 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 390 CCTCAGG CC^T[ACGT]AGG
ApoI 148 GAATTC [GA]^AATT[CT]
Asp700I 775 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 62 GGCGCC G^G[CT][GA]CC
BanII 425 GGGCTC G[GA]GC[CT]^C
BbeI 62 GGCGCC GGCGC^C
BbrPI 292 CACGTG CAC^GTG
BcgI 511 CGAGAACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 172 CCGGG CC^[GC]GG
BsaAI 292 CACGTG [CT]AC^GT[GA]
BsaHI 62 GGCGCC G[GA]^CG[CT]C
BsaI 337 GAGACC GGTCTC(1/5)
BsrFI 41 GCCGGC [GA]^CCGG[CT]
Bsu36I 390 CCTCAGG CC^T[ACGT]AGG
Cfr10I 41 GCCGGC [GA]^CCGG[CT]
DraII 658 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 56 CCGTGG C^C[GA][CT]GG
EarI 681 CTCTTC CTCTTC(1/4)
Eco81I 390 CCTCAGG CC^T[ACGT]AGG
EcoNI 342 CCTGATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 658 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 148 GAATTC G^AATTC
EcoT14I 522 CCTTGG C^C[AT][AT]GG
HaeIII 119 GGCC GG^CC
Hin1I 62 GGCGCC G[GA]^CG[CT]C
HindIII 792 AAGCTT A^AGCTT
HphI 133 GGTGA GGTGA(8/7)
KasI 62 GGCGCC G^GCGCC
Ksp632I 681 CTCTTC CTCTTC(1/4)
MaeII 293 ACGT A^CGT
MaeIII 158 GTCAC ^GT[ACGT]AC
NaeI 41 GCCGGC GCC^GGC
NarI 62 GGCGCC GG^CGCC
NciI 172 CCGGG CC^[GC]GG
NgoMIV 41 GCCGGC G^CCGGC
NspI 502 GCATGT [GA]CATG^[CT]
PflMI 376 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 292 CACGTG CAC^GTG
PmlI 292 CACGTG CAC^GTG
PpuMI 658 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 418 CAGCTG CAG^CTG
SfoI 62 GGCGCC GGC^GCC
StyI 522 CCTTGG C^C[AT][AT]GG
TaqI 194 TCGA T^CGA
TfiI 89 GATTC G^A[AT]TC
Tsp45I 158 GTCAC ^GT[GC]AC
TthHB8I 194 TCGA T^CGA
Van91I 376 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 775 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 256 GTAC GT^AC
465 GTAC
AspHI 79 GTGCTC G[AT]GC[AT]^C
708 GAGCAC
AvaII 659 GGACC G^G[AT]CC
823 GGACC
BlpI 415 GCTCAGC GC^T[ACGT]AGC
794 GCTTAGC
BpmI 122 CTGGAG CTGGAG(16/14)
439 CTGGAG
Bpu1102I 415 GCTCAGC GC^T[ACGT]AGC
794 GCTTAGC
BsiHKAI 79 GTGCTC G[AT]GC[AT]^C
708 GAGCAC
BsmAI 337 GAGAC GTCTC(1/5)
605 GTCTC
BspMI 535 ACCTGC ACCTGC(4/8)
8 GCAGGT
BstXI 50 CCACCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
468 CCACCTGTATGG
CelII 415 GCTCAGC GC^T[ACGT]AGC
794 GCTTAGC
DpnII 297 GATC ^GATC
345 GATC
FauI 216 CCCGC CCCGC(4/6)
548 CCCGC
HapII 42 CCGG C^CGG
172 CCGG
HpaII 42 CCGG C^CGG
172 CCGG
MboI 297 GATC ^GATC
345 GATC
MspI 42 CCGG C^CGG
172 CCGG
NdeII 297 GATC ^GATC
345 GATC
NlaIII 503 CATG CATG^
582 CATG
PleI 125 GAGTC GAGTC(4/5)
590 GAGTC
RsaI 256 GTAC GT^AC
465 GTAC
Sau3AI 297 GATC ^GATC
345 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI BbsI BbvI BmyI
BpuAI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
Fnu4HI HaeII HhaI HinP1I HinfI
ItaI MboII MnlI MslI MspA1I
MvaI MwoI NlaIV Sau96I ScrFI
SfaNI TseI Tsp509I TspRI