##################################
# NO CUT ENZYMES (  216 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstBI       BstEII      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeII       MaeIII      MamI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        ScrFI       SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   10 enzymes) #
###################################

BfaI          458  CTAG                 C^TAG
BseRI         309  CTCCTC               GAGGAG(10/8)
BsgI          282  GTGCAG               GTGCAG(16/14)
BsmFI         469  GTCCC                GGGAC(10/14)
BstAPI        437  GCAATCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII         492  GATC                 ^GATC
MaeI          458  CTAG                 C^TAG
MboI          492  GATC                 ^GATC
NdeII         492  GATC                 ^GATC
Sau3AI        492  GATC                 ^GATC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AocI          311  CCTCAGG              CC^T[ACGT]AGG
              398  CCTCAGG             
BanII         183  GGGCTC               G[GA]GC[CT]^C
              213  GGGCTC              
BpmI           96  CTCCAG               CTGGAG(16/14)
              162  CTCCAG              
BsaJI         188  CCAAGG               C^C[ACGT][ACGT]GG
              218  CCAAGG              
BsiYI          35  CCGGCTGTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               56  CCGGCTGTGGG         
BslI           35  CCGGCTGTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               56  CCGGCTGTGGG         
Bsu36I        311  CCTCAGG              CC^T[ACGT]AGG
              398  CCTCAGG             
Cac8I         125  GCAAGC               GC[ACGT]^[ACGT]GC
              147  GCCAGC              
Eco81I        311  CCTCAGG              CC^T[ACGT]AGG
              398  CCTCAGG             
EcoT14I       188  CCAAGG               C^C[AT][AT]GG
              218  CCAAGG              
HapII          35  CCGG                 C^CGG
               56  CCGG                
HpaII          35  CCGG                 C^CGG
               56  CCGG                
MspI           35  CCGG                 C^CGG
               56  CCGG                
StyI          188  CCAAGG               C^C[AT][AT]GG
              218  CCAAGG              
TspRI         280  CAGTG                CA[GC]TG[ACGT][ACGT]^
              484  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AluI        AlwNI       BbvI        BmyI        Bsp1286I    
BsrI        BstF5I      DdeI        Fnu4HI      FokI        
ItaI        MnlI        MspA1I      MwoI        NlaIV       
PvuII       TseI