##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BseRI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssKI       BssSI       Bst1107I    BstBI       
BstEII      BstNI       BstPI       BstXI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        HphI        KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MluI        MluNI       MscI        MseI        MslI        
MunI        MvaI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccII         669  CGCG                 CG^CG
AccIII         19  TCCGGA               T^CCGGA
AocI          737  CCTCAGG              CC^T[ACGT]AGG
AspHI         483  GTGCTC               G[AT]GC[AT]^C
BsaWI          19  TCCGGA               [AT]^CCGG[AT]
BseAI          19  TCCGGA               T^CCGGA
BsgI          708  GTGCAG               GTGCAG(16/14)
BsiHKAI       483  GTGCTC               G[AT]GC[AT]^C
BsmFI         808  GTCCC                GGGAC(10/14)
BspEI          19  TCCGGA               T^CCGGA
BstAPI        776  GCAAGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         669  CGCG                 CG^CG
Bsu36I        737  CCTCAGG              CC^T[ACGT]AGG
CfoI          668  GCGC                 GCG^C
Cfr13I        494  GGGCC                G^G[ACGT]CC
DraII         493  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EarI          210  CTCTTC               CTCTTC(1/4)
Eco81I        737  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      493  GGGGCCT              [GA]G^G[ACGT]CC[CT]
HaeIII        495  GGCC                 GG^CC
HhaI          668  GCGC                 GCG^C
HinP1I        668  GCGC                 G^CGC
Ksp632I       210  CTCTTC               CTCTTC(1/4)
MboII         211  TCTTC                GAAGA(8/7)
MroI           19  TCCGGA               T^CCGGA
MvnI          669  CGCG                 CG^CG
NlaIII        417  CATG                 CATG^
SapI          209  GCTCTTC              GCTCTTC(1/4)
Sau96I        494  GGGCC                G^G[ACGT]CC


###################################
# TWO CUT ENZYMES (    3 enzymes) #
###################################

DdeI          677  CTAAG                C^T[ACGT]AG
              738  CTCAG               
FauI          670  GCGGG                CCCGC(4/6)
              689  GCGGG               
TspRI         706  CAGTG                CA[GC]TG[ACGT][ACGT]^
              823  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       BamHI       
BanII       BbvI        BmyI        BpmI        BsaJI       
BsiYI       BslI        Bsp1286I    BsrI        BstF5I      
BstYI       Cac8I       DpnII       EcoT14I     Fnu4HI      
FokI        HapII       HpaII       ItaI        MboI        
MflI        MnlI        MspA1I      MspI        MwoI        
NdeII       NlaIV       PvuII       Sau3AI      StyI        
TseI        XhoII