Restriction map for
pF1KE0592
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccIII AclI AflII
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII BbvCI BcgI
BciVI BfrI BlpI Bpu1102I BsaBI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstXI
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HindIII HpaI KpnI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PleI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1087 GTATAC GT^[AC][GT]AC
AccII 1260 CGCG CG^CG
AflIII 977 ACACGT A^C[GA][CT]GT
AgeI 1241 ACCGGT A^CCGGT
Asp700I 579 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 326 GAGCTC G[AT]GC[AT]^C
AvrII 1311 CCTAGG C^CTAGG
BamHI 1337 GGATCC G^GATCC
BbrPI 998 CACGTG CAC^GTG
BclI 362 TGATCA T^GATCA
BglI 956 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1376 AGATCT A^GATCT
BlnI 1311 CCTAGG C^CTAGG
BpmI 1040 CTCCAG CTGGAG(16/14)
BsaAI 998 CACGTG [CT]AC^GT[GA]
BsaWI 1241 ACCGGT [AT]^CCGG[AT]
BsiHKAI 326 GAGCTC G[AT]GC[AT]^C
BspHI 725 TCATGA T^CATGA
BspMI 791 ACCTGC ACCTGC(4/8)
BsrDI 546 CATTGC GCAATG(2/0)
BsrFI 1241 ACCGGT [GA]^CCGG[CT]
Bst1107I 1087 GTATAC GTA^TAC
BstUI 1260 CGCG CG^CG
BstZ17I 1087 GTATAC GTA^TAC
Cfr10I 1241 ACCGGT [GA]^CCGG[CT]
EcoNI 687 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 809 CCCGC CCCGC(4/6)
FbaI 362 TGATCA T^GATCA
HincII 601 GTCAAC GT[CT]^[GA]AC
HindII 601 GTCAAC GT[CT]^[GA]AC
KspI 1259 CCGCGG CCGC^GG
MslI 222 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1260 CGCG CG^CG
NcoI 1223 CCATGG C^CATGG
PinAI 1241 ACCGGT A^CCGGT
PmaCI 998 CACGTG CAC^GTG
PmlI 998 CACGTG CAC^GTG
PpuMI 413 GGGACCC [GA]G^G[AT]CC[CT]
PstI 877 CTGCAG CTGCA^G
RcaI 725 TCATGA T^CATGA
SacI 326 GAGCTC GAGCT^C
SacII 1259 CCGCGG CCGC^GG
ScaI 1367 AGTACT AGT^ACT
SexAI 967 ACCTGGT A^CC[AT]GGT
SfaNI 1280 GCATC GCATC(5/9)
SfcI 877 CTGCAG C^T[GA][CT]AG
XcmI 345 CCAAAAGTTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 579 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 286 GGCGCC G[GA]^CG[CT]C
1315 GGCGCC
AspI 673 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1069 GACCCGGTC
BalI 659 TGGCCA TGG^CCA
819 TGGCCA
BbeI 286 GGCGCC GGCGC^C
1315 GGCGCC
BsaHI 286 GGCGCC G[GA]^CG[CT]C
1315 GGCGCC
BsaI 1216 GAGACC GGTCTC(1/5)
1143 GGTCTC
BsmAI 1216 GAGAC GTCTC(1/5)
1144 GTCTC
BsmFI 413 GGGAC GGGAC(10/14)
1035 GTCCC
BsmI 583 GCATTC GAATGC(1/-1)
1117 GCATTC
CfoI 287 GCGC GCG^C
1316 GCGC
HaeII 286 GGCGCC [GA]GCGC^[CT]
1315 GGCGCC
HhaI 287 GCGC GCG^C
1316 GCGC
Hin1I 286 GGCGCC G[GA]^CG[CT]C
1315 GGCGCC
HinP1I 287 GCGC G^CGC
1316 GCGC
KasI 286 GGCGCC G^GCGCC
1315 GGCGCC
MluNI 659 TGGCCA TGG^CCA
819 TGGCCA
MscI 659 TGGCCA TGG^CCA
819 TGGCCA
NarI 286 GGCGCC GG^CGCC
1315 GGCGCC
PflFI 673 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1069 GACCCGGTC
PvuII 173 CAGCTG CAG^CTG
1177 CAGCTG
SapI 846 GCTCTTC GCTCTTC(1/4)
1422 GCTCTTC
SfoI 286 GGCGCC GGC^GCC
1315 GGCGCC
Tth111I 673 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1069 GACCCGGTC
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI RsaI Sau3AI Sau96I
ScrFI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII