Restriction map for
pF1KE0624
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DdeI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MseI MunI MvnI NarI NdeI
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I Tsp45I
Tsp509I Tth111I XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AcyI 349 GACGCC G[GA]^CG[CT]C
Asp700I 786 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 510 CCCGGG C^[CT]CG[GA]G
BalI 137 TGGCCA TGG^CCA
BanI 964 GGCACC G^G[CT][GA]CC
BanII 304 GAGCTC G[GA]GC[CT]^C
BbsI 460 GAAGAC GAAGAC(2/6)
BciVI 547 GGATAC GTATCC(6/5)
BfaI 844 CTAG C^TAG
BglI 154 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 166 CTGGAG CTGGAG(16/14)
BpuAI 460 GAAGAC GAAGAC(2/6)
BsaAI 550 TACGTG [CT]AC^GT[GA]
BsaHI 349 GACGCC G[GA]^CG[CT]C
BsgI 208 CTGCAC GTGCAG(16/14)
BsoBI 510 CCCGGG C^[CT]CG[GA]G
Bsp1407I 411 TGTACA T^GTACA
BspHI 473 TCATGA T^CATGA
BspMI 392 ACCTGC ACCTGC(4/8)
BsrBI 343 GAGCGG CCGCTC(-3/-3)
BsrDI 150 CATTGC GCAATG(2/0)
BsrFI 1032 GCCGGC [GA]^CCGG[CT]
BsrGI 411 TGTACA T^GTACA
BstAPI 804 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 640 GGTTACC G^GT[ACGT]ACC
BstPI 640 GGTTACC G^GT[ACGT]ACC
BstXI 926 CCACGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 420 GGATCT [GA]^GATC[CT]
Cfr10I 1032 GCCGGC [GA]^CCGG[CT]
EaeI 137 TGGCCA [CT]^GGCC[GA]
EcoO65I 640 GGTTACC G^GT[ACGT]ACC
EcoT22I 147 ATGCAT ATGCA^T
FauI 345 GCGGG CCCGC(4/6)
Hin1I 349 GACGCC G[GA]^CG[CT]C
MaeI 844 CTAG C^TAG
MaeII 551 ACGT A^CGT
MaeIII 641 GTTAC ^GT[ACGT]AC
MflI 420 GGATCT [GA]^GATC[CT]
MluNI 137 TGGCCA TGG^CCA
MscI 137 TGGCCA TGG^CCA
MslI 927 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1032 GCCGGC GCC^GGC
NgoMIV 1032 GCCGGC G^CCGGC
NsiI 147 ATGCAT ATGCA^T
PstI 955 CTGCAG CTGCA^G
RcaI 473 TCATGA T^CATGA
SacI 304 GAGCTC GAGCT^C
SmaI 510 CCCGGG CCC^GGG
SmlI 424 CTCAAG C^T[CT][GA]AG
SspBI 411 TGTACA T^GTACA
StuI 806 AGGCCT AGG^CCT
TaqI 1053 TCGA T^CGA
TthHB8I 1053 TCGA T^CGA
XbaI 843 TCTAGA T^CTAGA
XcmI 155 CCAAGGAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 420 GGATCT [GA]^GATC[CT]
XmaCI 510 CCCGGG C^CCGGG
XmaI 510 CCCGGG C^CCGGG
XmnI 786 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwI 102 GATCC GGATC(4/5)
420 GGATC
AspHI 304 GAGCTC G[AT]GC[AT]^C
520 GTGCTC
BcgI 660 CGAGAATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1054 CGAGACTGCTGC
BsiHKAI 304 GAGCTC G[AT]GC[AT]^C
520 GTGCTC
BsmAI 50 GAGAC GTCTC(1/5)
1055 GAGAC
HgaI 123 GACGC GACGC(5/10)
349 GACGC
NcoI 820 CCATGG C^CATGG
999 CCATGG
PflMI 369 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1018 CCAGTGGCTGG
PpuMI 506 AGGACCC [GA]G^G[AT]CC[CT]
776 AGGACCC
SfaNI 379 GATGC GCATC(5/9)
667 GATGC
TfiI 435 GAATC G^A[AT]TC
791 GATTC
Van91I 369 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1018 CCAGTGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DpnII
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI StyI TseI TspRI