Restriction map for
pF1KE0650
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AgeI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BciVI BclI
BfaI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
CelII CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MflI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp509I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AflII 547 CTTAAG C^TTAAG
AflIII 671 ACATGT A^C[GA][CT]GT
AhdI 670 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 631 CCTGAGG CC^T[ACGT]AGG
Aor51HI 332 AGCGCT AGC^GCT
ApaI 30 GGGCCC GGGCC^C
AspEI 670 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 77 GTGCTC G[AT]GC[AT]^C
AspI 985 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1016 TGGCCA TGG^CCA
BcgI 920 CGATGGTAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 547 CTTAAG C^TTAAG
BglI 782 GCCAGTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 175 CTGGAG CTGGAG(16/14)
BsaI 577 GAGACC GGTCTC(1/5)
BsgI 469 CTGCAC GTGCAG(16/14)
BsiHKAI 77 GTGCTC G[AT]GC[AT]^C
BsmI 355 GAATGC GAATGC(1/-1)
Bsp1407I 695 TGTACA T^GTACA
BspDI 918 ATCGAT AT^CGAT
BspHI 893 TCATGA T^CATGA
BspLU11I 671 ACATGT A^CATGT
BspMI 141 GCAGGT ACCTGC(4/8)
BsrDI 483 CATTGC GCAATG(2/0)
BsrFI 1076 ACCGGC [GA]^CCGG[CT]
BsrGI 695 TGTACA T^GTACA
BstXI 1007 CCAGTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 631 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1076 ACCGGC [GA]^CCGG[CT]
ClaI 918 ATCGAT AT^CGAT
DsaI 324 CCATGG C^C[GA][CT]GG
EaeI 1016 TGGCCA [CT]^GGCC[GA]
Eam1105I 670 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 762 GAAGAG CTCTTC(1/4)
Eco47III 332 AGCGCT AGC^GCT
Eco81I 631 CCTGAGG CC^T[ACGT]AGG
EcoT22I 357 ATGCAT ATGCA^T
HaeII 332 AGCGCT [GA]GCGC^[CT]
HgaI 538 GACGC GACGC(5/10)
Ksp632I 762 GAAGAG CTCTTC(1/4)
MluNI 1016 TGGCCA TGG^CCA
MscI 1016 TGGCCA TGG^CCA
MseI 548 TTAA T^TAA
MspA1I 835 CAGCTG C[AC]G^C[GT]G
NcoI 324 CCATGG C^CATGG
NsiI 357 ATGCAT ATGCA^T
PflFI 985 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 915 CCAATCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 835 CAGCTG CAG^CTG
RcaI 893 TCATGA T^CATGA
SexAI 1040 ACCAGGT A^CC[AT]GGT
SmaI 753 CCCGGG CCC^GGG
SmlI 547 CTTAAG C^T[CT][GA]AG
SspBI 695 TGTACA T^GTACA
TaqI 919 TCGA T^CGA
Tru9I 548 TTAA T^TAA
Tth111I 985 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 919 TCGA T^CGA
Van91I 915 CCAATCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 753 CCCGGG C^CCGGG
XmaI 753 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 253 GTAGAC GT^[AC][GT]AC
1030 GTAGAC
AlwI 840 GATCC GGATC(4/5)
908 GGATC
AlwNI 142 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
463 CAGTTTCTG
AvaI 753 CCCGGG C^[CT]CG[GA]G
953 CCCGAG
BanII 30 GGGCCC G[GA]GC[CT]^C
97 GAGCCC
BbsI 855 GAAGAC GAAGAC(2/6)
1062 GAAGAC
BpuAI 855 GAAGAC GAAGAC(2/6)
1062 GAAGAC
BsmFI 983 GGGAC GGGAC(10/14)
678 GTCCC
BsoBI 753 CCCGGG C^[CT]CG[GA]G
953 CCCGAG
CfoI 192 GCGC GCG^C
333 GCGC
HhaI 192 GCGC GCG^C
333 GCGC
HinP1I 192 GCGC G^CGC
333 GCGC
HinfI 387 GATTC G^A[ACGT]TC
771 GATTC
MaeIII 115 GTGAC ^GT[ACGT]AC
668 GTGAC
MslI 345 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
490 CACCGAGATG
NspI 317 ACATGC [GA]CATG^[CT]
671 ACATGT
PpuMI 635 AGGTCCT [GA]G^G[AT]CC[CT]
1055 AGGACCT
SfaNI 826 GATGC GCATC(5/9)
296 GCATC
TfiI 387 GATTC G^A[AT]TC
771 GATTC
Tsp45I 115 GTGAC ^GT[GC]AC
668 GTGAC
XcmI 137 CCAAGCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
186 CCATCGGCGCACTGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
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AciI AfaI AluI AvaII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TseI TspRI