Restriction map for
pF1KE0669
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AlwI 702 GATCC GGATC(4/5)
AspHI 573 GAGCAC G[AT]GC[AT]^C
BanI 639 GGCACC G^G[CT][GA]CC
BcgI 147 CGAGGACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 522 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 726 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 726 GCTCAGC GC^T[ACGT]AGC
BseRI 223 GAGGAG GAGGAG(10/8)
BsiHKAI 573 GAGCAC G[AT]GC[AT]^C
Bsp1407I 71 TGTACA T^GTACA
BspMI 344 ACCTGC ACCTGC(4/8)
BsrGI 71 TGTACA T^GTACA
BssHII 262 GCGCGC G^CGCGC
BssSI 682 CACGAG CACGAG(-5/-1)
BstYI 701 AGATCC [GA]^GATC[CT]
CelII 726 GCTCAGC GC^T[ACGT]AGC
DpnII 702 GATC ^GATC
EarI 136 CTCTTC CTCTTC(1/4)
HaeII 107 GGCGCT [GA]GCGC^[CT]
HinfI 288 GAGTC G^A[ACGT]TC
Ksp632I 136 CTCTTC CTCTTC(1/4)
MaeIII 41 GTGAC ^GT[ACGT]AC
MboI 702 GATC ^GATC
MboII 137 TCTTC GAAGA(8/7)
MflI 701 AGATCC [GA]^GATC[CT]
MslI 267 CACCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 527 GCCGGC GCC^GGC
NcoI 299 CCATGG C^CATGG
NdeII 702 GATC ^GATC
NgoMIV 527 GCCGGC G^CCGGC
NspI 131 ACATGC [GA]CATG^[CT]
PleI 288 GAGTC GAGTC(4/5)
PpuMI 253 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 376 CAGCTG CAG^CTG
SapI 135 GCTCTTC GCTCTTC(1/4)
Sau3AI 702 GATC ^GATC
SfaNI 295 GATGC GCATC(5/9)
SgrAI 103 CACCGGCG C[GA]^CCGG[CT]G
SmaI 257 CCCGGG CCC^GGG
SspBI 71 TGTACA T^GTACA
StuI 592 AGGCCT AGG^CCT
Tsp45I 41 GTGAC ^GT[GC]AC
XhoII 701 AGATCC [GA]^GATC[CT]
XmaCI 257 CCCGGG C^CCGGG
XmaI 257 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 43 GACGCC G[GA]^CG[CT]C
175 GGCGTC
AfaI 72 GTAC GT^AC
388 GTAC
AlwNI 373 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
430 CAGTCCCTG
ApaI 649 GGGCCC GGGCC^C
707 GGGCCC
AvaI 257 CCCGGG C^[CT]CG[GA]G
601 CCCGAG
BalI 693 TGGCCA TGG^CCA
717 TGGCCA
BsaAI 271 TACGTG [CT]AC^GT[GA]
389 TACGTG
BsaHI 43 GACGCC G[GA]^CG[CT]C
175 GGCGTC
BsgI 195 CTGCAC GTGCAG(16/14)
679 CTGCAC
BsoBI 257 CCCGGG C^[CT]CG[GA]G
601 CCCGAG
BsrFI 104 ACCGGC [GA]^CCGG[CT]
527 GCCGGC
Cfr10I 104 ACCGGC [GA]^CCGG[CT]
527 GCCGGC
EcoT14I 299 CCATGG C^C[AT][AT]GG
548 CCAAGG
Hin1I 43 GACGCC G[GA]^CG[CT]C
175 GGCGTC
MaeII 272 ACGT A^CGT
390 ACGT
MluNI 693 TGGCCA TGG^CCA
717 TGGCCA
MscI 693 TGGCCA TGG^CCA
717 TGGCCA
MspA1I 281 CCGCTG C[AC]G^C[GT]G
376 CAGCTG
RsaI 72 GTAC GT^AC
388 GTAC
StyI 299 CCATGG C^C[AT][AT]GG
548 CCAAGG
TaqI 146 TCGA T^CGA
338 TCGA
TthHB8I 146 TCGA T^CGA
338 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI MnlI MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI TseI TspRI