Restriction map for
pF1KE0676
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglII
BlnI BpmI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsiYI BslI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cac8I
CfoI Cfr10I CpoI DraI DraII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 350 GTCTAC GT^[AC][GT]AC
AflII 474 CTTAAG C^TTAAG
AspHI 36 GAGCTC G[AT]GC[AT]^C
BanII 36 GAGCTC G[GA]GC[CT]^C
BfaI 925 CTAG C^TAG
BfrI 474 CTTAAG C^TTAAG
BglI 131 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 468 GCTAAGC GC^T[ACGT]AGC
BmyI 36 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 468 GCTAAGC GC^T[ACGT]AGC
BsaAI 959 TACGTG [CT]AC^GT[GA]
BseRI 1141 GAGGAG GAGGAG(10/8)
BsiHKAI 36 GAGCTC G[AT]GC[AT]^C
BsmAI 48 GAGAC GTCTC(1/5)
Bsp1286I 36 GAGCTC G[AGT]GC[ACT]^C
BspDI 688 ATCGAT AT^CGAT
BstF5I 1002 GGATG GGATG(2/0)
BstXI 899 CCAAATACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 798 AGATCC [GA]^GATC[CT]
CelII 468 GCTAAGC GC^T[ACGT]AGC
ClaI 688 ATCGAT AT^CGAT
DraIII 197 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 529 GATATC GAT^ATC
FokI 1002 GGATG GGATG(9/13)
HindIII 471 AAGCTT A^AGCTT
MaeI 925 CTAG C^TAG
MflI 798 AGATCC [GA]^GATC[CT]
SacI 36 GAGCTC GAGCT^C
SfcI 989 CTATAG C^T[GA][CT]AG
SspI 841 AATATT AAT^ATT
StuI 536 AGGCCT AGG^CCT
TaqI 689 TCGA T^CGA
TthHB8I 689 TCGA T^CGA
XhoII 798 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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Asp700I 496 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
619 GAAGGAGTTC
AvaII 232 GGACC G^G[AT]CC
1017 GGACC
BalI 129 TGGCCA TGG^CCA
768 TGGCCA
BbsI 29 GAAGAC GAAGAC(2/6)
643 GAAGAC
BpuAI 29 GAAGAC GAAGAC(2/6)
643 GAAGAC
BsrI 766 ACTGG ACTGG(1/-1)
221 CCAGT
EaeI 129 TGGCCA [CT]^GGCC[GA]
768 TGGCCA
EcoT14I 132 CCAAGG C^C[AT][AT]GG
778 CCTTGG
HphI 724 GGTGA GGTGA(8/7)
889 GGTGA
MaeII 720 ACGT A^CGT
960 ACGT
MluNI 129 TGGCCA TGG^CCA
768 TGGCCA
MscI 129 TGGCCA TGG^CCA
768 TGGCCA
MseI 250 TTAA T^TAA
475 TTAA
MwoI 16 GCAGCTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
131 GCCAAGGAGGC
PleI 178 GAGTC GAGTC(4/5)
1178 GAGTC
SmlI 20 CTCAAG C^T[CT][GA]AG
474 CTTAAG
StyI 132 CCAAGG C^C[AT][AT]GG
778 CCTTGG
Tru9I 250 TTAA T^TAA
475 TTAA
XcmI 132 CCAAGGAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
756 CCAATGGCTTACTGG
XmnI 496 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
619 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BsaJI BssKI BssSI BstNI
Cfr13I DdeI DpnII EcoRI EcoRII
Fnu4HI HaeIII HinfI ItaI MaeIII
MboI MboII MnlI MslI MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI TfiI
TseI Tsp45I Tsp509I TspRI