Restriction map for
pF1KE0680
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AgeI AhdI Alw44I
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BanI BbeI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsiWI BsmBI BsmI BspDI BspHI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI DraI
DrdI Eam1105I EarI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MluI MseI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI TaqI
Tru9I Tsp45I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 36 TCCGGA T^CCGGA
AcyI 10 GGCGTC G[GA]^CG[CT]C
AflIII 548 ACACGT A^C[GA][CT]GT
Aor51HI 632 AGCGCT AGC^GCT
Asp700I 207 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 7 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 641 TGGCCA TGG^CCA
BamHI 236 GGATCC G^GATCC
BbrPI 549 CACGTG CAC^GTG
BbsI 819 GAAGAC GAAGAC(2/6)
BpuAI 819 GAAGAC GAAGAC(2/6)
BsaAI 549 CACGTG [CT]AC^GT[GA]
BsaHI 10 GGCGTC G[GA]^CG[CT]C
BseAI 36 TCCGGA T^CCGGA
BsgI 421 GTGCAG GTGCAG(16/14)
BsiEI 1026 CGGCCG CG[GA][CT]^CG
BsmAI 593 GTCTC GTCTC(1/5)
Bsp1407I 299 TGTACA T^GTACA
BspEI 36 TCCGGA T^CCGGA
BspMI 674 ACCTGC ACCTGC(4/8)
BsrBI 43 GAGCGG CCGCTC(-3/-3)
BsrDI 196 GCAATG GCAATG(2/0)
BsrGI 299 TGTACA T^GTACA
BstAPI 78 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 779 CGGACCG CG^G[AT]CCG
DraIII 1048 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 881 CCACGG C^C[GA][CT]GG
EagI 1026 CGGCCG C^GGCCG
EclXI 1026 CGGCCG C^GGCCG
Eco47III 632 AGCGCT AGC^GCT
Eco52I 1026 CGGCCG C^GGCCG
HgaI 11 GCGTC GACGC(5/10)
Hin1I 10 GGCGTC G[GA]^CG[CT]C
MluNI 641 TGGCCA TGG^CCA
MroI 36 TCCGGA T^CCGGA
MscI 641 TGGCCA TGG^CCA
PflFI 7 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 240 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 33 GACTC GAGTC(4/5)
PmaCI 549 CACGTG CAC^GTG
PmlI 549 CACGTG CAC^GTG
PpuMI 671 GGGACCT [GA]G^G[AT]CC[CT]
PstI 265 CTGCAG CTGCA^G
RsrII 779 CGGACCG CG^G[AT]CCG
SacI 115 GAGCTC GAGCT^C
SfcI 265 CTGCAG C^T[GA][CT]AG
SspBI 299 TGTACA T^GTACA
StuI 494 AGGCCT AGG^CCT
Tsp509I 477 AATT ^AATT
Tth111I 7 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 240 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 128 CCATGAGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 207 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AlwNI 79 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
100 CAGCGCCTG
AspHI 115 GAGCTC G[AT]GC[AT]^C
373 GTGCTC
AvaI 277 CCCGAG C^[CT]CG[GA]G
896 CCCGAG
BsiHKAI 115 GAGCTC G[AT]GC[AT]^C
373 GTGCTC
BsoBI 277 CCCGAG C^[CT]CG[GA]G
896 CCCGAG
DraII 425 AGGCCCT [GA]G^G[ACGT]CC[CT]
671 GGGACCT
EaeI 641 TGGCCA [CT]^GGCC[GA]
1026 CGGCCG
EcoO109I 425 AGGCCCT [GA]G^G[ACGT]CC[CT]
671 GGGACCT
HphI 816 GGTGA GGTGA(8/7)
404 TCACC
MaeII 550 ACGT A^CGT
823 ACGT
TspRI 120 CAGTG CA[GC]TG[ACGT][ACGT]^
371 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsaWI BseRI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII Eco81I
EcoNI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI XhoII