Restriction map for
pF1KE0682
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BcgI BciVI BclI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstYI BstZ17I Cfr10I ClaI
CpoI DraI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 775 GTCTAC GT^[AC][GT]AC
AcyI 446 GGCGCC G[GA]^CG[CT]C
AfaI 645 GTAC GT^AC
AvaI 221 CCCGGG C^[CT]CG[GA]G
BbeI 446 GGCGCC GGCGC^C
BbrPI 451 CACGTG CAC^GTG
BbsI 237 GAAGAC GAAGAC(2/6)
BbvCI 757 CCTCAGC CCTCAGC(-5/-2)
BfaI 1112 CTAG C^TAG
BglI 136 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 522 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 522 GCTCAGC GC^T[ACGT]AGC
BpuAI 237 GAAGAC GAAGAC(2/6)
BsaAI 451 CACGTG [CT]AC^GT[GA]
BsaHI 446 GGCGCC G[GA]^CG[CT]C
BsaI 217 GAGACC GGTCTC(1/5)
BsgI 502 CTGCAC GTGCAG(16/14)
BsmBI 538 GAGACG CGTCTC(1/5)
BsoBI 221 CCCGGG C^[CT]CG[GA]G
BspMI 494 ACCTGC ACCTGC(4/8)
BsrBI 520 CCGCTC CCGCTC(-3/-3)
BstEII 375 GGTGACC G^GT[ACGT]ACC
BstPI 375 GGTGACC G^GT[ACGT]ACC
BstXI 1075 CCACCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 522 GCTCAGC GC^T[ACGT]AGC
DpnII 75 GATC ^GATC
DraIII 451 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 969 GAAGAG CTCTTC(1/4)
EcoO65I 375 GGTGACC G^GT[ACGT]ACC
HgaI 540 GACGC GACGC(5/10)
Hin1I 446 GGCGCC G[GA]^CG[CT]C
KasI 446 GGCGCC G^GCGCC
Ksp632I 969 GAAGAG CTCTTC(1/4)
MaeI 1112 CTAG C^TAG
MaeII 452 ACGT A^CGT
MboI 75 GATC ^GATC
NarI 446 GGCGCC GG^CGCC
NdeII 75 GATC ^GATC
PflMI 102 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 451 CACGTG CAC^GTG
PmlI 451 CACGTG CAC^GTG
RsaI 645 GTAC GT^AC
SapI 969 GAAGAGC GCTCTTC(1/4)
Sau3AI 75 GATC ^GATC
SfoI 446 GGCGCC GGC^GCC
SmaI 221 CCCGGG CCC^GGG
StuI 982 AGGCCT AGG^CCT
TfiI 604 GAATC G^A[AT]TC
Van91I 102 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1111 TCTAGA T^CTAGA
XcmI 999 CCAGAATACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 221 CCCGGG C^CCGGG
XmaI 221 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 337 CCTCAGG CC^T[ACGT]AGG
591 CCTGAGG
BseRI 1160 CTCCTC GAGGAG(10/8)
178 GAGGAG
BsmI 162 GAATGC GAATGC(1/-1)
1023 GCATTC
BstF5I 51 CATCC GGATG(2/0)
744 CATCC
Bsu36I 337 CCTCAGG CC^T[ACGT]AGG
591 CCTGAGG
DsaI 288 CCATGG C^C[GA][CT]GG
872 CCATGG
Eco81I 337 CCTCAGG CC^T[ACGT]AGG
591 CCTGAGG
EcoNI 187 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
985 CCTGTCCCAGG
FokI 51 CATCC GGATG(9/13)
744 CATCC
HaeII 446 GGCGCC [GA]GCGC^[CT]
472 AGCGCC
MaeIII 38 GTCAC ^GT[ACGT]AC
376 GTGAC
MslI 78 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
909 CATCTATGTG
NcoI 288 CCATGG C^CATGG
872 CCATGG
PleI 567 GACTC GAGTC(4/5)
36 GAGTC
PpuMI 491 AGGACCT [GA]G^G[AT]CC[CT]
629 AGGACCC
SbfI 207 CCTGCAGG CCTGCA^GG
577 CCTGCAGG
SfaNI 684 GATGC GCATC(5/9)
124 GCATC
Sse8387I 207 CCTGCAGG CCTGCA^GG
577 CCTGCAGG
Tsp45I 38 GTCAC ^GT[GC]AC
376 GTGAC
TspRI 117 CACTG CA[GC]TG[ACGT][ACGT]^
250 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstAPI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PstI PvuII Sau96I ScrFI SfcI
StyI TseI