Restriction map for
pF1KE0709
##################################
# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BclI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI Fnu4HI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI ItaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TseI Tth111I Van91I XbaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 214 GTAGAC GT^[AC][GT]AC
AciI 105 GCGG CCGC(-3/-1)
AflIII 380 ACGTGT A^C[GA][CT]GT
AluI 362 AGCT AG^CT
AocI 603 CCTGAGG CC^T[ACGT]AGG
AseI 96 ATTAAT AT^TAAT
AsnI 96 ATTAAT AT^TAAT
AvaI 779 CTCGAG C^[CT]CG[GA]G
BbsI 303 GAAGAC GAAGAC(2/6)
BciVI 112 GGATAC GTATCC(6/5)
BfaI 424 CTAG C^TAG
BpmI 571 CTCCAG CTGGAG(16/14)
BpuAI 303 GAAGAC GAAGAC(2/6)
BsmFI 546 GTCCC GGGAC(10/14)
BsoBI 779 CTCGAG C^[CT]CG[GA]G
BsrDI 879 CATTGC GCAATG(2/0)
BstF5I 507 GGATG GGATG(2/0)
BstYI 38 GGATCT [GA]^GATC[CT]
Bsu36I 603 CCTGAGG CC^T[ACGT]AGG
Cac8I 525 GCCAGC GC[ACGT]^[ACGT]GC
DsaI 470 CCGTGG C^C[GA][CT]GG
Eco81I 603 CCTGAGG CC^T[ACGT]AGG
EcoRI 240 GAATTC G^AATTC
FokI 507 GGATG GGATG(9/13)
HaeIII 608 GGCC GG^CC
HincII 196 GTTGAC GT[CT]^[GA]AC
HindII 196 GTTGAC GT[CT]^[GA]AC
HphI 269 TCACC GGTGA(8/7)
MaeI 424 CTAG C^TAG
MflI 38 GGATCT [GA]^GATC[CT]
MslI 797 CATTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV 486 GGAACC GG[ACGT]^[ACGT]CC
PaeR7I 779 CTCGAG C^TCGAG
PleI 777 GACTC GAGTC(4/5)
PshBI 96 ATTAAT AT^TAAT
SfaNI 567 GATGC GCATC(5/9)
SmlI 779 CTCGAG C^T[CT][GA]AG
StuI 607 AGGCCT AGG^CCT
TaqI 780 TCGA T^CGA
TfiI 668 GAATC G^A[AT]TC
TthHB8I 780 TCGA T^CGA
XhoI 779 CTCGAG C^TCGAG
XhoII 38 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 600 GATCC GGATC(4/5)
38 GGATC
BcnI 117 CCGGG CC^[GC]GG
548 CCCGG
BsaJI 394 CCTGGG C^C[ACGT][ACGT]GG
470 CCGTGG
BsmAI 451 GAGAC GTCTC(1/5)
775 GAGAC
DpnII 39 GATC ^GATC
600 GATC
HapII 117 CCGG C^CGG
549 CCGG
HinfI 668 GAATC G^A[ACGT]TC
777 GACTC
HpaII 117 CCGG C^CGG
549 CCGG
MaeII 380 ACGT A^CGT
466 ACGT
MboI 39 GATC ^GATC
600 GATC
MspI 117 CCGG C^CGG
549 CCGG
NciI 117 CCGGG CC^[GC]GG
548 CCCGG
NdeII 39 GATC ^GATC
600 GATC
NspI 223 ACATGC [GA]CATG^[CT]
529 GCATGT
Sau3AI 39 GATC ^GATC
600 GATC
Tsp45I 384 GTCAC ^GT[GC]AC
617 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI AfaI ApoI BsiYI BslI
BsrI BssKI BstNI DdeI EcoRII
MaeIII MboII MnlI MseI MvaI
NlaIII RsaI ScrFI Tru9I Tsp509I
TspRI