Restriction map for
pF1KE0717
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII Eco81I
EcoO109I EcoO65I EcoRII EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI MvaI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 6 GAATTC [GA]^AATT[CT]
AcyI 184 GACGCC G[GA]^CG[CT]C
AhdI 394 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 566 GATCC GGATC(4/5)
AlwNI 834 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 134 AGCGCT AGC^GCT
ApaI 796 GGGCCC GGGCC^C
ApoI 6 GAATTC [GA]^AATT[CT]
AspEI 394 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 346 GAGCAC G[AT]GC[AT]^C
AvaII 862 GGACC G^G[AT]CC
BanII 796 GGGCCC G[GA]GC[CT]^C
BbvCI 328 GCTGAGG CCTCAGC(-5/-2)
BcgI 381 CGAGATTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 184 GACGCC G[GA]^CG[CT]C
BsaI 611 GAGACC GGTCTC(1/5)
BsaWI 541 TCCGGT [AT]^CCGG[AT]
BseRI 175 GAGGAG GAGGAG(10/8)
BsgI 46 GTGCAG GTGCAG(16/14)
BsiHKAI 346 GAGCAC G[AT]GC[AT]^C
BsmBI 320 GAGACG CGTCTC(1/5)
BsrBI 807 GAGCGG CCGCTC(-3/-3)
BstF5I 481 CATCC GGATG(2/0)
DrdI 51 GACGAGTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 394 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 804 GAAGAG CTCTTC(1/4)
Eco47III 134 AGCGCT AGC^GCT
EcoRI 6 GAATTC G^AATTC
EcoRV 532 GATATC GAT^ATC
FauI 240 GCGGG CCCGC(4/6)
FokI 481 CATCC GGATG(9/13)
HaeII 134 AGCGCT [GA]GCGC^[CT]
Hin1I 184 GACGCC G[GA]^CG[CT]C
Ksp632I 804 GAAGAG CTCTTC(1/4)
KspI 464 CCGCGG CCGC^GG
MaeII 323 ACGT A^CGT
MspA1I 464 CCGCGG C[AC]G^C[GT]G
NcoI 191 CCATGG C^CATGG
SacII 464 CCGCGG CCGC^GG
SapI 804 GAAGAGC GCTCTTC(1/4)
SfaNI 780 GCATC GCATC(5/9)
SfcI 771 CTACAG C^T[GA][CT]AG
Tsp45I 157 GTGAC ^GT[GC]AC
Tsp509I 7 AATT ^AATT
TspRI 225 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AvaI 251 CCCGGG C^[CT]CG[GA]G
572 CCCGGG
BanI 274 GGTGCC G^G[CT][GA]CC
364 GGCACC
BbvI 767 GCAGC GCAGC(8/12)
905 GCAGC
BsmAI 320 GAGAC GTCTC(1/5)
611 GAGAC
BsoBI 251 CCCGGG C^[CT]CG[GA]G
572 CCCGGG
DdeI 11 CTCAG C^T[ACGT]AG
329 CTGAG
DpnII 342 GATC ^GATC
566 GATC
EagI 579 CGGCCG C^GGCCG
588 CGGCCG
EclXI 579 CGGCCG C^GGCCG
588 CGGCCG
Eco52I 579 CGGCCG C^GGCCG
588 CGGCCG
EcoNI 624 CCTTTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
865 CCTTACAGAGG
HgaI 184 GACGC GACGC(5/10)
448 GACGC
HphI 117 GGTGA GGTGA(8/7)
507 GGTGA
MboI 342 GATC ^GATC
566 GATC
MboII 510 GAAGA GAAGA(8/7)
804 GAAGA
MseI 290 TTAA T^TAA
314 TTAA
NdeII 342 GATC ^GATC
566 GATC
PflMI 207 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
495 CCATCGCGTGG
PleI 93 GACTC GAGTC(4/5)
58 GAGTC
Sau3AI 342 GATC ^GATC
566 GATC
SfiI 64 GGCCTGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
580 GGCCGAGGCGGCC
SmaI 251 CCCGGG CCC^GGG
572 CCCGGG
StuI 83 AGGCCT AGG^CCT
309 AGGCCT
TaqI 344 TCGA T^CGA
609 TCGA
Tru9I 290 TTAA T^TAA
314 TTAA
TseI 767 GCAGC G^C[AT]GC
905 GCAGC
TthHB8I 344 TCGA T^CGA
609 TCGA
Van91I 207 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
495 CCATCGCGTGG
XmaCI 251 CCCGGG C^CCGGG
572 CCCGGG
XmaI 251 CCCGGG C^CCGGG
572 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AluI BcnI BglI
BmyI BsaJI BsiEI BsiYI BslI
BsmFI Bsp1286I BssKI BstUI Cac8I
CfoI Cfr13I DsaI EaeI EcoT14I
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeIII MnlI
MspI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI StyI TfiI