################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcsI 130 AAATTT [GA]^AATT[CT] AfaI 801 GTAC GT^AC AflIII 419 ACGTGT A^C[GA][CT]GT Alw44I 887 GTGCAC G^TGCAC ApaLI 887 GTGCAC G^TGCAC ApoI 130 AAATTT [GA]^AATT[CT] AseI 708 ATTAAT AT^TAAT AsnI 708 ATTAAT AT^TAAT Asp700I 127 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 887 GTGCAC G[AT]GC[AT]^C AspI 19 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 215 CCCGAG C^[CT]CG[GA]G BalI 683 TGGCCA TGG^CCA BanII 367 GAGCCC G[GA]GC[CT]^C BbsI 267 GAAGAC GAAGAC(2/6) BcnI 734 CCCGG CC^[GC]GG BlpI 691 GCTGAGC GC^T[ACGT]AGC Bpu1102I 691 GCTGAGC GC^T[ACGT]AGC BpuAI 267 GAAGAC GAAGAC(2/6) BsaAI 37 TACGTG [CT]AC^GT[GA] BsiEI 561 CGGTCG CG[GA][CT]^CG BsiHKAI 887 GTGCAC G[AT]GC[AT]^C BsmI 460 GAATGC GAATGC(1/-1) BsoBI 215 CCCGAG C^[CT]CG[GA]G BspHI 480 TCATGA T^CATGA BssSI 884 CTCGTG CACGAG(-5/-1) BstF5I 987 CATCC GGATG(2/0) CelII 691 GCTGAGC GC^T[ACGT]AGC CfoI 797 GCGC GCG^C DraII 111 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 111 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 502 GATATC GAT^ATC FauI 396 GCGGG CCCGC(4/6) FokI 987 CATCC GGATG(9/13) HapII 735 CCGG C^CGG HhaI 797 GCGC GCG^C HinP1I 797 GCGC G^CGC HpaII 735 CCGG C^CGG MluNI 683 TGGCCA TGG^CCA MscI 683 TGGCCA TGG^CCA MspA1I 394 CAGCGG C[AC]G^C[GT]G MspI 735 CCGG C^CGG NciI 734 CCCGG CC^[GC]GG NspI 231 GCATGC [GA]CATG^[CT] PflFI 19 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 111 AGGACCC [GA]G^G[AT]CC[CT] PshBI 708 ATTAAT AT^TAAT RcaI 480 TCATGA T^CATGA RsaI 801 GTAC GT^AC SphI 231 GCATGC GCATG^C TaqI 265 TCGA T^CGA Tsp45I 605 GTGAC ^GT[GC]AC Tsp509I 131 AATT ^AATT Tth111I 19 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 265 TCGA T^CGA XcmI 515 CCAAGGTCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 127 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccII 798 CGCG CG^CG 947 CGCG BfaI 104 CTAG C^TAG 715 CTAG BmyI 367 GAGCCC G[AGT]GC[ACT]^C 887 GTGCAC BpmI 978 CTCCAG CTGGAG(16/14) 292 CTGGAG BseRI 301 GAGGAG GAGGAG(10/8) 552 GAGGAG BsgI 184 CTGCAC GTGCAG(16/14) 391 GTGCAG BsiYI 299 CCGAGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 318 CCTCTGCATGG BslI 299 CCGAGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 318 CCTCTGCATGG BsmAI 220 GAGAC GTCTC(1/5) 882 GTCTC BsmFI 337 GGGAC GGGAC(10/14) 566 GGGAC Bsp1286I 367 GAGCCC G[AGT]GC[ACT]^C 887 GTGCAC BstNI 341 CCAGG CC^[AT]GG 444 CCTGG BstUI 798 CGCG CG^CG 947 CGCG Cac8I 231 GCATGC GC[ACGT]^[ACGT]GC 311 GCAAGC DpnII 474 GATC ^GATC 654 GATC EaeI 380 TGGCCG [CT]^GGCC[GA] 683 TGGCCA EarI 785 CTCTTC CTCTTC(1/4) 925 GAAGAG EcoRII 341 CCAGG ^CC[AT]GG 444 CCTGG EcoT14I 360 CCTTGG C^C[AT][AT]GG 515 CCAAGG HindIII 58 AAGCTT A^AGCTT 747 AAGCTT Ksp632I 785 CTCTTC CTCTTC(1/4) 925 GAAGAG MaeI 104 CTAG C^TAG 715 CTAG MaeIII 175 GTAAC ^GT[ACGT]AC 605 GTGAC MboI 474 GATC ^GATC 654 GATC MseI 14 TTAA T^TAA 709 TTAA MvaI 341 CCAGG CC^[AT]GG 444 CCTGG MvnI 798 CGCG CG^CG 947 CGCG MwoI 676 GCGACACTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 939 GCCGTTGTCGC NdeII 474 GATC ^GATC 654 GATC Sau3AI 474 GATC ^GATC 654 GATC SfaNI 77 GATGC GCATC(5/9) 807 GCATC SfcI 843 CTACAG C^T[GA][CT]AG 972 CTACAG StyI 360 CCTTGG C^C[AT][AT]GG 515 CCAAGG TfiI 413 GATTC G^A[AT]TC 965 GATTC Tru9I 14 TTAA T^TAA 709 TTAA TspRI 548 CACTG CA[GC]TG[ACGT][ACGT]^ 680 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AluI AvaII BbvI BsaJI BsrI BssKI Cfr13I DdeI Fnu4HI HaeIII HinfI HphI ItaI MaeII MboII MnlI NlaIII NlaIV PleI Sau96I ScrFI TseI