Restriction map for pF1KE0759
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AlwI        
AlwNI       AocI        Aor51HI     ApaI        AscI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MroI        MslI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

AcsI          130  AAATTT               [GA]^AATT[CT]
AfaI          801  GTAC                 GT^AC
AflIII        419  ACGTGT               A^C[GA][CT]GT
Alw44I        887  GTGCAC               G^TGCAC
ApaLI         887  GTGCAC               G^TGCAC
ApoI          130  AAATTT               [GA]^AATT[CT]
AseI          708  ATTAAT               AT^TAAT
AsnI          708  ATTAAT               AT^TAAT
Asp700I       127  GAAAAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         887  GTGCAC               G[AT]GC[AT]^C
AspI           19  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          215  CCCGAG               C^[CT]CG[GA]G
BalI          683  TGGCCA               TGG^CCA
BanII         367  GAGCCC               G[GA]GC[CT]^C
BbsI          267  GAAGAC               GAAGAC(2/6)
BcnI          734  CCCGG                CC^[GC]GG
BlpI          691  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      691  GCTGAGC              GC^T[ACGT]AGC
BpuAI         267  GAAGAC               GAAGAC(2/6)
BsaAI          37  TACGTG               [CT]AC^GT[GA]
BsiEI         561  CGGTCG               CG[GA][CT]^CG
BsiHKAI       887  GTGCAC               G[AT]GC[AT]^C
BsmI          460  GAATGC               GAATGC(1/-1)
BsoBI         215  CCCGAG               C^[CT]CG[GA]G
BspHI         480  TCATGA               T^CATGA
BssSI         884  CTCGTG               CACGAG(-5/-1)
BstF5I        987  CATCC                GGATG(2/0)
CelII         691  GCTGAGC              GC^T[ACGT]AGC
CfoI          797  GCGC                 GCG^C
DraII         111  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      111  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRV         502  GATATC               GAT^ATC
FauI          396  GCGGG                CCCGC(4/6)
FokI          987  CATCC                GGATG(9/13)
HapII         735  CCGG                 C^CGG
HhaI          797  GCGC                 GCG^C
HinP1I        797  GCGC                 G^CGC
HpaII         735  CCGG                 C^CGG
MluNI         683  TGGCCA               TGG^CCA
MscI          683  TGGCCA               TGG^CCA
MspA1I        394  CAGCGG               C[AC]G^C[GT]G
MspI          735  CCGG                 C^CGG
NciI          734  CCCGG                CC^[GC]GG
NspI          231  GCATGC               [GA]CATG^[CT]
PflFI          19  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         111  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI         708  ATTAAT               AT^TAAT
RcaI          480  TCATGA               T^CATGA
RsaI          801  GTAC                 GT^AC
SphI          231  GCATGC               GCATG^C
TaqI          265  TCGA                 T^CGA
Tsp45I        605  GTGAC                ^GT[GC]AC
Tsp509I       131  AATT                 ^AATT
Tth111I        19  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       265  TCGA                 T^CGA
XcmI          515  CCAAGGTCAAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          127  GAAAAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AccII         798  CGCG                 CG^CG
              947  CGCG                
BfaI          104  CTAG                 C^TAG
              715  CTAG                
BmyI          367  GAGCCC               G[AGT]GC[ACT]^C
              887  GTGCAC              
BpmI          978  CTCCAG               CTGGAG(16/14)
              292  CTGGAG              
BseRI         301  GAGGAG               GAGGAG(10/8)
              552  GAGGAG              
BsgI          184  CTGCAC               GTGCAG(16/14)
              391  GTGCAG              
BsiYI         299  CCGAGGAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              318  CCTCTGCATGG         
BslI          299  CCGAGGAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              318  CCTCTGCATGG         
BsmAI         220  GAGAC                GTCTC(1/5)
              882  GTCTC               
BsmFI         337  GGGAC                GGGAC(10/14)
              566  GGGAC               
Bsp1286I      367  GAGCCC               G[AGT]GC[ACT]^C
              887  GTGCAC              
BstNI         341  CCAGG                CC^[AT]GG
              444  CCTGG               
BstUI         798  CGCG                 CG^CG
              947  CGCG                
Cac8I         231  GCATGC               GC[ACGT]^[ACGT]GC
              311  GCAAGC              
DpnII         474  GATC                 ^GATC
              654  GATC                
EaeI          380  TGGCCG               [CT]^GGCC[GA]
              683  TGGCCA              
EarI          785  CTCTTC               CTCTTC(1/4)
              925  GAAGAG              
EcoRII        341  CCAGG                ^CC[AT]GG
              444  CCTGG               
EcoT14I       360  CCTTGG               C^C[AT][AT]GG
              515  CCAAGG              
HindIII        58  AAGCTT               A^AGCTT
              747  AAGCTT              
Ksp632I       785  CTCTTC               CTCTTC(1/4)
              925  GAAGAG              
MaeI          104  CTAG                 C^TAG
              715  CTAG                
MaeIII        175  GTAAC                ^GT[ACGT]AC
              605  GTGAC               
MboI          474  GATC                 ^GATC
              654  GATC                
MseI           14  TTAA                 T^TAA
              709  TTAA                
MvaI          341  CCAGG                CC^[AT]GG
              444  CCTGG               
MvnI          798  CGCG                 CG^CG
              947  CGCG                
MwoI          676  GCGACACTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              939  GCCGTTGTCGC         
NdeII         474  GATC                 ^GATC
              654  GATC                
Sau3AI        474  GATC                 ^GATC
              654  GATC                
SfaNI          77  GATGC                GCATC(5/9)
              807  GCATC               
SfcI          843  CTACAG               C^T[GA][CT]AG
              972  CTACAG              
StyI          360  CCTTGG               C^C[AT][AT]GG
              515  CCAAGG              
TfiI          413  GATTC                G^A[AT]TC
              965  GATTC               
Tru9I          14  TTAA                 T^TAA
              709  TTAA                
TspRI         548  CACTG                CA[GC]TG[ACGT][ACGT]^
              680  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BsaJI       
BsrI        BssKI       Cfr13I      DdeI        Fnu4HI      
HaeIII      HinfI       HphI        ItaI        MaeII       
MboII       MnlI        NlaIII      NlaIV       PleI        
Sau96I      ScrFI       TseI        
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