Restriction map for
pF1KE0777
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I CpoI
DdeI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TfiI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AcsI 756 GAATTC [GA]^AATT[CT]
AlwNI 695 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 756 GAATTC [GA]^AATT[CT]
Asp700I 91 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcnI 331 CCGGG CC^[GC]GG
BfaI 38 CTAG C^TAG
BglII 260 AGATCT A^GATCT
BmyI 244 GGGCAC G[AGT]GC[ACT]^C
BpmI 767 CTGGAG CTGGAG(16/14)
BsgI 184 GTGCAG GTGCAG(16/14)
BsiEI 328 CGACCG CG[GA][CT]^CG
Bsp1286I 244 GGGCAC G[AGT]GC[ACT]^C
BspDI 734 ATCGAT AT^CGAT
BsrDI 585 CATTGC GCAATG(2/0)
BstYI 260 AGATCT [GA]^GATC[CT]
ClaI 734 ATCGAT AT^CGAT
DsaI 921 CCGTGG C^C[GA][CT]GG
EaeI 918 TGGCCG [CT]^GGCC[GA]
EcoRI 756 GAATTC G^AATTC
HapII 331 CCGG C^CGG
HgaI 34 GACGC GACGC(5/10)
HpaII 331 CCGG C^CGG
MaeI 38 CTAG C^TAG
MflI 260 AGATCT [GA]^GATC[CT]
MspI 331 CCGG C^CGG
NciI 331 CCGGG CC^[GC]GG
NlaIV 333 GGGACC GG[ACGT]^[ACGT]CC
NspI 725 ACATGC [GA]CATG^[CT]
StuI 176 AGGCCT AGG^CCT
TaqI 735 TCGA T^CGA
Tsp45I 679 GTGAC ^GT[GC]AC
TthHB8I 735 TCGA T^CGA
XhoII 260 AGATCT [GA]^GATC[CT]
XmnI 91 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 773 GCGG CCGC(-3/-1)
959 GCGG
AlwI 301 GATCC GGATC(4/5)
844 GGATC
AvaII 25 GGTCC G^G[AT]CC
334 GGACC
BbvI 80 GCAGC GCAGC(8/12)
700 GCTGC
BsaJI 156 CCAGGG C^C[ACGT][ACGT]GG
921 CCGTGG
BsiYI 349 CCAATAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
766 CCTGGAGGCGG
BslI 349 CCAATAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
766 CCTGGAGGCGG
BstAPI 208 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
694 GCAGTAGCTGC
BstF5I 976 GGATG GGATG(2/0)
986 GGATG
BstNI 156 CCAGG CC^[AT]GG
766 CCTGG
Cfr13I 25 GGTCC G^G[ACGT]CC
334 GGACC
EcoRII 156 CCAGG ^CC[AT]GG
766 CCTGG
FokI 976 GGATG GGATG(9/13)
986 GGATG
HaeIII 177 GGCC GG^CC
919 GGCC
HphI 508 GGTGA GGTGA(8/7)
601 TCACC
MaeIII 679 GTGAC ^GT[ACGT]AC
944 GTTAC
MslI 490 CATGGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
685 CATGAAGATG
MvaI 156 CCAGG CC^[AT]GG
766 CCTGG
NdeI 338 CATATG CA^TATG
547 CATATG
Sau96I 25 GGTCC G^G[ACGT]CC
334 GGACC
SfaNI 511 GATGC GCATC(5/9)
691 GATGC
TseI 80 GCAGC G^C[AT]GC
700 GCTGC
TspRI 879 CAGTG CA[GC]TG[ACGT][ACGT]^
936 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
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AfaI AluI BsmFI BssKI DpnII
EarI Fnu4HI ItaI Ksp632I MboI
MboII MnlI MwoI NdeII NlaIII
RsaI Sau3AI ScrFI Tsp509I