Restriction map for
pF1KE0782
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AgeI Alw44I AlwNI
ApaI ApaLI AscI AseI AsnI
Asp718I AviII AvrII BamHI BbsI
BbvCI BciVI BfrI BglII BlnI
BpuAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DraII
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Van91I
XbaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AhdI 667 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 696 GATCC GGATC(4/5)
AocI 1345 CCTGAGG CC^T[ACGT]AGG
Asp700I 1059 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 667 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 472 GAGCTC G[AT]GC[AT]^C
AspI 1281 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 770 TGGCCA TGG^CCA
BbrPI 571 CACGTG CAC^GTG
BclI 554 TGATCA T^GATCA
BfaI 1246 CTAG C^TAG
BlpI 396 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 396 GCTGAGC GC^T[ACGT]AGC
BsaI 385 GGTCTC GGTCTC(1/5)
BsgI 619 GTGCAG GTGCAG(16/14)
BsiEI 740 CGGCCG CG[GA][CT]^CG
BsiHKAI 472 GAGCTC G[AT]GC[AT]^C
BspMI 746 ACCTGC ACCTGC(4/8)
BsrFI 547 GCCGGC [GA]^CCGG[CT]
BstAPI 143 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 695 AGATCC [GA]^GATC[CT]
Bsu36I 1345 CCTGAGG CC^T[ACGT]AGG
CelII 396 GCTGAGC GC^T[ACGT]AGC
Cfr10I 547 GCCGGC [GA]^CCGG[CT]
DrdI 1216 GACATCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 458 CCGTGG C^C[GA][CT]GG
EagI 740 CGGCCG C^GGCCG
Eam1105I 667 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 740 CGGCCG C^GGCCG
Eco52I 740 CGGCCG C^GGCCG
Eco81I 1345 CCTGAGG CC^T[ACGT]AGG
EcoNI 1296 CCTACTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1179 GAATTC G^AATTC
FbaI 554 TGATCA T^GATCA
FseI 546 GGCCGGCC GGCCGG^CC
HincII 1231 GTCAAC GT[CT]^[GA]AC
HindII 1231 GTCAAC GT[CT]^[GA]AC
MaeI 1246 CTAG C^TAG
MflI 695 AGATCC [GA]^GATC[CT]
MluNI 770 TGGCCA TGG^CCA
MscI 770 TGGCCA TGG^CCA
NaeI 547 GCCGGC GCC^GGC
NgoMIV 547 GCCGGC G^CCGGC
PaeR7I 1254 CTCGAG C^TCGAG
PflFI 1281 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 571 CACGTG CAC^GTG
PmlI 571 CACGTG CAC^GTG
SacI 472 GAGCTC GAGCT^C
SapI 474 GCTCTTC GCTCTTC(1/4)
SfiI 24 GGCCGCGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 1281 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 563 CCAATGCCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1254 CTCGAG C^TCGAG
XhoII 695 AGATCC [GA]^GATC[CT]
XmnI 1059 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1154 GAATTT [GA]^AATT[CT]
1179 GAATTC
AflIII 89 ACGTGT A^C[GA][CT]GT
296 ACGTGT
Aor51HI 12 AGCGCT AGC^GCT
1061 AGCGCT
ApoI 1154 GAATTT [GA]^AATT[CT]
1179 GAATTC
AvaII 462 GGTCC G^G[AT]CC
919 GGACC
BbeI 66 GGCGCC GGCGC^C
370 GGCGCC
BcgI 1256 CGAGTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
779 GCAGTCCCTTCG
BpmI 220 CTGGAG CTGGAG(16/14)
1300 CTGGAG
BsaAI 127 TACGTG [CT]AC^GT[GA]
571 CACGTG
BseRI 1289 CTCCTC GAGGAG(10/8)
1327 CTCCTC
BsrDI 426 CATTGC GCAATG(2/0)
681 CATTGC
DraIII 259 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
571 CACGTGGTG
EarI 475 CTCTTC CTCTTC(1/4)
1330 CTCTTC
Eco47III 12 AGCGCT AGC^GCT
1061 AGCGCT
HgaI 345 GACGC GACGC(5/10)
1239 GCGTC
KasI 66 GGCGCC G^GCGCC
370 GGCGCC
Ksp632I 475 CTCTTC CTCTTC(1/4)
1330 CTCTTC
MslI 283 CACACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1074 CATACGGATG
NarI 66 GGCGCC GG^CGCC
370 GGCGCC
PvuII 358 CAGCTG CAG^CTG
394 CAGCTG
SfoI 66 GGCGCC GGC^GCC
370 GGCGCC
SmaI 50 CCCGGG CCC^GGG
597 CCCGGG
TaqI 665 TCGA T^CGA
1255 TCGA
Tsp509I 1155 AATT ^AATT
1180 AATT
TthHB8I 665 TCGA T^CGA
1255 TCGA
XmaCI 50 CCCGGG C^CCGGG
597 CCCGGG
XmaI 50 CCCGGG C^CCGGG
597 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BanI BanII BbvI BcnI BglI
BmyI BsaHI BsaJI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TseI Tsp45I TspRI