################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiHKAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 909 CGCG CG^CG AflIII 303 ACACGT A^C[GA][CT]GT AlwNI 446 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 904 GGGCCC GGGCC^C BbsI 543 GAAGAC GAAGAC(2/6) BfaI 440 CTAG C^TAG BglI 291 GCCCAAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 820 GCTCAGC GC^T[ACGT]AGC Bpu1102I 820 GCTCAGC GC^T[ACGT]AGC BpuAI 543 GAAGAC GAAGAC(2/6) BsaWI 323 TCCGGT [AT]^CCGG[AT] BsmAI 307 GTCTC GTCTC(1/5) BsmBI 306 CGTCTC CGTCTC(1/5) Bsp1407I 140 TGTACA T^GTACA BsrFI 646 ACCGGC [GA]^CCGG[CT] BsrGI 140 TGTACA T^GTACA BstNI 172 CCAGG CC^[AT]GG BstUI 909 CGCG CG^CG CelII 820 GCTCAGC GC^T[ACGT]AGC Cfr10I 646 ACCGGC [GA]^CCGG[CT] EaeI 102 CGGCCA [CT]^GGCC[GA] EcoRI 610 GAATTC G^AATTC EcoRII 172 CCAGG ^CC[AT]GG EcoT14I 410 CCAAGG C^C[AT][AT]GG HinfI 866 GAGTC G^A[ACGT]TC KspI 908 CCGCGG CCGC^GG MaeI 440 CTAG C^TAG MseI 395 TTAA T^TAA MvaI 172 CCAGG CC^[AT]GG MvnI 909 CGCG CG^CG PflMI 563 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 866 GAGTC GAGTC(4/5) PshAI 108 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 830 CTGCAG CTGCA^G PvuI 482 CGATCG CGAT^CG PvuII 875 CAGCTG CAG^CTG SacII 908 CCGCGG CCGC^GG SmaI 461 CCCGGG CCC^GGG SspBI 140 TGTACA T^GTACA StyI 410 CCAAGG C^C[AT][AT]GG Tru9I 395 TTAA T^TAA Tsp45I 622 GTGAC ^GT[GC]AC Van91I 563 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 461 CCCGGG C^CCGGG XmaI 461 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccI 598 GTCTAC GT^[AC][GT]AC 868 GTCTAC AcsI 371 AAATTT [GA]^AATT[CT] 610 GAATTC ApoI 371 AAATTT [GA]^AATT[CT] 610 GAATTC BsiEI 482 CGATCG CG[GA][CT]^CG 755 CGGTCG BsrI 24 CCAGT ACTGG(1/-1) 583 CCAGT DpnII 387 GATC ^GATC 483 GATC DraII 205 GGGACCC [GA]G^G[ACGT]CC[CT] 332 GGGACCC DsaI 518 CCACGG C^C[GA][CT]GG 908 CCGCGG EcoO109I 205 GGGACCC [GA]G^G[ACGT]CC[CT] 332 GGGACCC MaeII 305 ACGT A^CGT 380 ACGT MboI 387 GATC ^GATC 483 GATC MboII 543 GAAGA GAAGA(8/7) 678 TCTTC NdeII 387 GATC ^GATC 483 GATC PpuMI 205 GGGACCC [GA]G^G[AT]CC[CT] 332 GGGACCC Sau3AI 387 GATC ^GATC 483 GATC SfaNI 448 GATGC GCATC(5/9) 50 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AfaI AluI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BssKI BstF5I Cac8I CfoI Cfr13I DdeI FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MnlI MspA1I MspI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfcI TseI Tsp509I TspRI