Restriction map for pF1KE0793
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BpmI        BsaAI       BsaBI       BsaHI       BsaI        
BseAI       BseRI       BsgI        BsiHKAI     BsiWI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        Ksp632I     MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TaqI        TfiI        Tth111I     
TthHB8I     XbaI        XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         909  CGCG                 CG^CG
AflIII        303  ACACGT               A^C[GA][CT]GT
AlwNI         446  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          904  GGGCCC               GGGCC^C
BbsI          543  GAAGAC               GAAGAC(2/6)
BfaI          440  CTAG                 C^TAG
BglI          291  GCCCAAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          820  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      820  GCTCAGC              GC^T[ACGT]AGC
BpuAI         543  GAAGAC               GAAGAC(2/6)
BsaWI         323  TCCGGT               [AT]^CCGG[AT]
BsmAI         307  GTCTC                GTCTC(1/5)
BsmBI         306  CGTCTC               CGTCTC(1/5)
Bsp1407I      140  TGTACA               T^GTACA
BsrFI         646  ACCGGC               [GA]^CCGG[CT]
BsrGI         140  TGTACA               T^GTACA
BstNI         172  CCAGG                CC^[AT]GG
BstUI         909  CGCG                 CG^CG
CelII         820  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        646  ACCGGC               [GA]^CCGG[CT]
EaeI          102  CGGCCA               [CT]^GGCC[GA]
EcoRI         610  GAATTC               G^AATTC
EcoRII        172  CCAGG                ^CC[AT]GG
EcoT14I       410  CCAAGG               C^C[AT][AT]GG
HinfI         866  GAGTC                G^A[ACGT]TC
KspI          908  CCGCGG               CCGC^GG
MaeI          440  CTAG                 C^TAG
MseI          395  TTAA                 T^TAA
MvaI          172  CCAGG                CC^[AT]GG
MvnI          909  CGCG                 CG^CG
PflMI         563  CCAACGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          866  GAGTC                GAGTC(4/5)
PshAI         108  GACCCCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          830  CTGCAG               CTGCA^G
PvuI          482  CGATCG               CGAT^CG
PvuII         875  CAGCTG               CAG^CTG
SacII         908  CCGCGG               CCGC^GG
SmaI          461  CCCGGG               CCC^GGG
SspBI         140  TGTACA               T^GTACA
StyI          410  CCAAGG               C^C[AT][AT]GG
Tru9I         395  TTAA                 T^TAA
Tsp45I        622  GTGAC                ^GT[GC]AC
Van91I        563  CCAACGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         461  CCCGGG               C^CCGGG
XmaI          461  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AccI          598  GTCTAC               GT^[AC][GT]AC
              868  GTCTAC              
AcsI          371  AAATTT               [GA]^AATT[CT]
              610  GAATTC              
ApoI          371  AAATTT               [GA]^AATT[CT]
              610  GAATTC              
BsiEI         482  CGATCG               CG[GA][CT]^CG
              755  CGGTCG              
BsrI           24  CCAGT                ACTGG(1/-1)
              583  CCAGT               
DpnII         387  GATC                 ^GATC
              483  GATC                
DraII         205  GGGACCC              [GA]G^G[ACGT]CC[CT]
              332  GGGACCC             
DsaI          518  CCACGG               C^C[GA][CT]GG
              908  CCGCGG              
EcoO109I      205  GGGACCC              [GA]G^G[ACGT]CC[CT]
              332  GGGACCC             
MaeII         305  ACGT                 A^CGT
              380  ACGT                
MboI          387  GATC                 ^GATC
              483  GATC                
MboII         543  GAAGA                GAAGA(8/7)
              678  TCTTC               
NdeII         387  GATC                 ^GATC
              483  GATC                
PpuMI         205  GGGACCC              [GA]G^G[AT]CC[CT]
              332  GGGACCC             
Sau3AI        387  GATC                 ^GATC
              483  GATC                
SfaNI         448  GATGC                GCATC(5/9)
               50  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AfaI        AluI        AvaI        AvaII       
BanII       BbvI        BcnI        BmyI        BsaJI       
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BsrBI       BssKI       BstF5I      Cac8I       CfoI        
Cfr13I      DdeI        FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MaeIII      MnlI        MspA1I      MspI        
MwoI        NciI        NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       SfcI        TseI        Tsp509I     
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com