Restriction map for
pF1KE0801
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MfeI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 934 AAATTT [GA]^AATT[CT]
AcyI 487 GGCGTC G[GA]^CG[CT]C
Alw44I 850 GTGCAC G^TGCAC
ApaLI 850 GTGCAC G^TGCAC
ApoI 934 AAATTT [GA]^AATT[CT]
AspHI 850 GTGCAC G[AT]GC[AT]^C
BalI 419 TGGCCA TGG^CCA
BamHI 494 GGATCC G^GATCC
BanII 60 GGGCTC G[GA]GC[CT]^C
BbsI 1047 GTCTTC GAAGAC(2/6)
BfaI 880 CTAG C^TAG
BpmI 1062 CTCCAG CTGGAG(16/14)
BpuAI 1047 GTCTTC GAAGAC(2/6)
BsaHI 487 GGCGTC G[GA]^CG[CT]C
BseRI 265 CTCCTC GAGGAG(10/8)
BsgI 244 CTGCAC GTGCAG(16/14)
BsiHKAI 850 GTGCAC G[AT]GC[AT]^C
BsmFI 68 GGGAC GGGAC(10/14)
BsrDI 176 GCAATG GCAATG(2/0)
BstAPI 295 GCAGTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 202 GGTTACC G^GT[ACGT]ACC
BstPI 202 GGTTACC G^GT[ACGT]ACC
Cac8I 954 GCACGC GC[ACGT]^[ACGT]GC
CfoI 714 GCGC GCG^C
DraI 937 TTTAAA TTT^AAA
DsaI 305 CCGTGG C^C[GA][CT]GG
EcoNI 333 CCTATGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 202 GGTTACC G^GT[ACGT]ACC
HgaI 488 GCGTC GACGC(5/10)
HhaI 714 GCGC GCG^C
Hin1I 487 GGCGTC G[GA]^CG[CT]C
HinP1I 714 GCGC G^CGC
HincII 364 GTCAAC GT[CT]^[GA]AC
HindII 364 GTCAAC GT[CT]^[GA]AC
MaeI 880 CTAG C^TAG
MaeII 539 ACGT A^CGT
MluNI 419 TGGCCA TGG^CCA
MscI 419 TGGCCA TGG^CCA
MslI 963 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PshAI 729 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 372 CTACAG C^T[GA][CT]AG
TaqI 794 TCGA T^CGA
TthHB8I 794 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 411 CCGC CCGC(-3/-1)
573 CCGC
AfaI 237 GTAC GT^AC
318 GTAC
AvaII 408 GGACC G^G[AT]CC
980 GGTCC
BbvI 243 GCTGC GCAGC(8/12)
768 GCTGC
BmyI 60 GGGCTC G[AGT]GC[ACT]^C
850 GTGCAC
BsaBI 786 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
861 GATTTCCATC
Bsp1286I 60 GGGCTC G[AGT]GC[ACT]^C
850 GTGCAC
BstYI 494 GGATCC [GA]^GATC[CT]
992 AGATCC
DraII 719 AGGCCCT [GA]G^G[ACGT]CC[CT]
875 AGGCCCT
EaeI 257 TGGCCG [CT]^GGCC[GA]
419 TGGCCA
EcoO109I 719 AGGCCCT [GA]G^G[ACGT]CC[CT]
875 AGGCCCT
Fnu4HI 243 GCTGC GC^[ACGT]GC
768 GCTGC
HphI 869 TCACC GGTGA(8/7)
962 TCACC
ItaI 243 GCTGC GC^[ACGT]GC
768 GCTGC
MamI 786 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
861 GATTTCCATC
MflI 494 GGATCC [GA]^GATC[CT]
992 AGATCC
MseI 160 TTAA T^TAA
938 TTAA
RsaI 237 GTAC GT^AC
318 GTAC
SfaNI 301 GATGC GCATC(5/9)
791 GCATC
SmlI 724 CTCAAG C^T[CT][GA]AG
988 CTCAAG
Tru9I 160 TTAA T^TAA
938 TTAA
TseI 243 GCTGC G^C[AT]GC
768 GCTGC
Tsp45I 569 GTGAC ^GT[GC]AC
690 GTCAC
Tsp509I 115 AATT ^AATT
935 AATT
XhoII 494 GGATCC [GA]^GATC[CT]
992 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI AlwI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII EcoT14I FokI
HaeIII HinfI MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
StyI TspRI