Restriction map for
pF1KE0805
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI AlwNI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsiEI
BsiWI BsmBI BsmFI Bsp1407I BspDI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FauI FbaI FseI HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MfeI MflI MluI MseI
MspA1I MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 1013 TCCGGA T^CCGGA
AcyI 628 GGCGTC G[GA]^CG[CT]C
AflIII 737 ACATGT A^C[GA][CT]GT
Alw44I 733 GTGCAC G^TGCAC
AlwI 91 GGATC GGATC(4/5)
ApaI 875 GGGCCC GGGCC^C
ApaLI 733 GTGCAC G^TGCAC
AvaI 235 CCCGAG C^[CT]CG[GA]G
AviII 1028 TGCGCA TGC^GCA
BanI 721 GGCACC G^G[CT][GA]CC
BbsI 223 GTCTTC GAAGAC(2/6)
BpuAI 223 GTCTTC GAAGAC(2/6)
BsaAI 730 TACGTG [CT]AC^GT[GA]
BsaHI 628 GGCGTC G[GA]^CG[CT]C
BsaI 391 GAGACC GGTCTC(1/5)
BsaWI 1013 TCCGGA [AT]^CCGG[AT]
BseAI 1013 TCCGGA T^CCGGA
BseRI 712 CTCCTC GAGGAG(10/8)
BsmI 114 GAATGC GAATGC(1/-1)
BsoBI 235 CCCGAG C^[CT]CG[GA]G
BspEI 1013 TCCGGA T^CCGGA
BspLU11I 737 ACATGT A^CATGT
BsrDI 109 GCAATG GCAATG(2/0)
BsrI 441 CCAGT ACTGG(1/-1)
BstAPI 109 GCAATGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 128 GCCTGC GC[ACGT]^[ACGT]GC
DrdI 532 GACAGGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 296 CCGTGG C^C[GA][CT]GG
EcoT22I 819 ATGCAT ATGCA^T
FspI 1028 TGCGCA TGC^GCA
HaeII 205 AGCGCC [GA]GCGC^[CT]
HapII 1014 CCGG C^CGG
HgaI 629 GCGTC GACGC(5/10)
Hin1I 628 GGCGTC G[GA]^CG[CT]C
HincII 529 GTCGAC GT[CT]^[GA]AC
HindII 529 GTCGAC GT[CT]^[GA]AC
HpaII 1014 CCGG C^CGG
MroI 1013 TCCGGA T^CCGGA
MspI 1014 CCGG C^CGG
NsiI 819 ATGCAT ATGCA^T
PflMI 450 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 472 GACTC GAGTC(4/5)
PstI 342 CTGCAG CTGCA^G
SalI 529 GTCGAC G^TCGAC
SfcI 342 CTGCAG C^T[GA][CT]AG
TfiI 1047 GATTC G^A[AT]TC
Tsp509I 1025 AATT ^AATT
Van91I 450 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 529 GTCGAC GT^[AC][GT]AC
652 GTCTAC
AccII 555 CGCG CG^CG
752 CGCG
AfaI 330 GTAC GT^AC
537 GTAC
AluI 58 AGCT AG^CT
86 AGCT
AspHI 166 GAGCAC G[AT]GC[AT]^C
733 GTGCAC
BalI 518 TGGCCA TGG^CCA
608 TGGCCA
BanII 79 GAGCCC G[GA]GC[CT]^C
875 GGGCCC
BsaBI 402 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
480 GATCTGCATC
BsgI 316 CTGCAC GTGCAG(16/14)
757 CTGCAC
BsiHKAI 166 GAGCAC G[AT]GC[AT]^C
733 GTGCAC
BsmAI 391 GAGAC GTCTC(1/5)
249 GTCTC
BspHI 398 TCATGA T^CATGA
575 TCATGA
BstF5I 6 CATCC GGATG(2/0)
279 CATCC
BstUI 555 CGCG CG^CG
752 CGCG
BstXI 380 CCAATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
845 CCATTCTCCTGG
DraII 590 AGGCCCT [GA]G^G[ACGT]CC[CT]
875 GGGCCCC
EcoO109I 590 AGGCCCT [GA]G^G[ACGT]CC[CT]
875 GGGCCCC
FokI 6 CATCC GGATG(9/13)
279 CATCC
HinfI 472 GACTC G^A[ACGT]TC
1047 GATTC
MaeIII 541 GTCAC ^GT[ACGT]AC
835 GTCAC
MamI 402 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
480 GATCTGCATC
MluNI 518 TGGCCA TGG^CCA
608 TGGCCA
MscI 518 TGGCCA TGG^CCA
608 TGGCCA
MslI 576 CATGACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
687 CATCACCATG
MvnI 555 CGCG CG^CG
752 CGCG
NspI 362 ACATGC [GA]CATG^[CT]
737 ACATGT
RcaI 398 TCATGA T^CATGA
575 TCATGA
RsaI 330 GTAC GT^AC
537 GTAC
SexAI 893 ACCTGGT A^CC[AT]GGT
962 ACCTGGT
Tsp45I 541 GTCAC ^GT[GC]AC
835 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AvaII BbvI BmyI BpmI
BsaJI BsiYI BslI Bsp1286I BspMI
BssKI BstNI CfoI Cfr13I DpnII
EaeI EcoRII Fnu4HI HaeIII HhaI
HinP1I HphI ItaI MaeII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I