Restriction map for
pF1KE0812
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 772 GTCTAC GT^[AC][GT]AC
AciI 363 CCGC CCGC(-3/-1)
AclI 604 AACGTT AA^CGTT
AcsI 818 AAATTC [GA]^AATT[CT]
AflII 675 CTTAAG C^TTAAG
ApaI 757 GGGCCC GGGCC^C
ApoI 818 AAATTC [GA]^AATT[CT]
AspHI 725 GTGCTC G[AT]GC[AT]^C
AspI 286 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 319 CTCGGG C^[CT]CG[GA]G
BanII 757 GGGCCC G[GA]GC[CT]^C
BbsI 150 GTCTTC GAAGAC(2/6)
BfaI 656 CTAG C^TAG
BfrI 675 CTTAAG C^TTAAG
BlpI 261 GCTGAGC GC^T[ACGT]AGC
BpmI 67 CTGGAG CTGGAG(16/14)
Bpu1102I 261 GCTGAGC GC^T[ACGT]AGC
BpuAI 150 GTCTTC GAAGAC(2/6)
BseRI 219 CTCCTC GAGGAG(10/8)
BsgI 108 CTGCAC GTGCAG(16/14)
BsiHKAI 725 GTGCTC G[AT]GC[AT]^C
BsoBI 319 CTCGGG C^[CT]CG[GA]G
BsrDI 735 CATTGC GCAATG(2/0)
BstAPI 326 GCATTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 512 CCAATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 416 GGATCT [GA]^GATC[CT]
CelII 261 GCTGAGC GC^T[ACGT]AGC
DsaI 809 CCGTGG C^C[GA][CT]GG
MaeI 656 CTAG C^TAG
MaeII 605 ACGT A^CGT
MflI 416 GGATCT [GA]^GATC[CT]
MseI 676 TTAA T^TAA
MslI 168 CACACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 326 GCATTGAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI 286 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 499 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 269 GACTC GAGTC(4/5)
Psp1406I 604 AACGTT AA^CGTT
SspI 903 AATATT AAT^ATT
Tru9I 676 TTAA T^TAA
Tth111I 286 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 499 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 791 CCAACCCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 416 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 686 GTAC GT^AC
814 GTAC
AluI 299 AGCT AG^CT
595 AGCT
AlwI 416 GGATC GGATC(4/5)
522 GGATC
AvaII 508 GGTCC G^G[AT]CC
838 GGACC
BsmFI 233 GTCCC GGGAC(10/14)
509 GTCCC
BsmI 458 GCATTC GAATGC(1/-1)
680 GCATTC
BspHI 404 TCATGA T^CATGA
824 TCATGA
BspMI 718 ACCTGC ACCTGC(4/8)
776 ACCTGC
BsrI 117 ACTGG ACTGG(1/-1)
467 CCAGT
BssKI 186 CCTGG ^CC[ACGT]GG
429 CCTGG
BstNI 186 CCTGG CC^[AT]GG
429 CCTGG
EcoRII 186 CCTGG ^CC[AT]GG
429 CCTGG
EcoT14I 412 CCAAGG C^C[AT][AT]GG
557 CCTTGG
MvaI 186 CCTGG CC^[AT]GG
429 CCTGG
PstI 720 CTGCAG CTGCA^G
778 CTGCAG
RcaI 404 TCATGA T^CATGA
824 TCATGA
RsaI 686 GTAC GT^AC
814 GTAC
ScrFI 186 CCTGG CC^[ACGT]GG
429 CCTGG
SfcI 720 CTGCAG C^T[GA][CT]AG
778 CTGCAG
SmlI 675 CTTAAG C^T[CT][GA]AG
867 CTTGAG
StyI 412 CCAAGG C^C[AT][AT]GG
557 CCTTGG
Tsp509I 639 AATT ^AATT
819 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BstF5I Cac8I Cfr13I DdeI
DpnII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MnlI NdeII NlaIII NlaIV Sau3AI
Sau96I TfiI TseI Tsp45I TspRI