Restriction map for
pF1KE0822
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# NO CUT ENZYMES ( 151 enzymes) #
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Acc65I AccI AccII AccIII AclI
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BcnI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BspHI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII CfoI ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FauI Fnu4HI FseI
FspI HaeII HgaI HhaI HinP1I
HincII HindII HpaI HphI ItaI
KasI KpnI KspI MamI MfeI
MflI MluI MroI MspA1I MunI
MvnI NarI NciI NcoI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI SwaI TaqI
TfiI TseI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 73 enzymes) #
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AatII 542 GACGTC GACGT^C
AciI 123 CCGC CCGC(-3/-1)
AcsI 9 AAATTT [GA]^AATT[CT]
AcyI 542 GACGTC G[GA]^CG[CT]C
AflII 240 CTTAAG C^TTAAG
AlwI 301 GATCC GGATC(4/5)
AocI 633 CCTCAGG CC^T[ACGT]AGG
ApoI 9 AAATTT [GA]^AATT[CT]
Asp700I 440 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 91 GAGCAC G[AT]GC[AT]^C
BalI 131 TGGCCA TGG^CCA
BbsI 27 GAAGAC GAAGAC(2/6)
BclI 395 TGATCA T^GATCA
BfaI 4 CTAG C^TAG
BfrI 240 CTTAAG C^TTAAG
BmyI 91 GAGCAC G[AGT]GC[ACT]^C
BpuAI 27 GAAGAC GAAGAC(2/6)
BsaAI 333 CACGTA [CT]AC^GT[GA]
BsaHI 542 GACGTC G[GA]^CG[CT]C
BseRI 305 CTCCTC GAGGAG(10/8)
BsiHKAI 91 GAGCAC G[AT]GC[AT]^C
BsiYI 123 CCGCTATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 123 CCGCTATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 540 GAGACG CGTCTC(1/5)
Bsp1286I 91 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 356 TGTACA T^GTACA
BspMI 43 GCAGGT ACCTGC(4/8)
BsrFI 367 GCCGGC [GA]^CCGG[CT]
BsrGI 356 TGTACA T^GTACA
BssKI 189 CCTGG ^CC[ACGT]GG
BstNI 189 CCTGG CC^[AT]GG
Bsu36I 633 CCTCAGG CC^T[ACGT]AGG
Cfr10I 367 GCCGGC [GA]^CCGG[CT]
DraII 244 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 560 CCGTGG C^C[GA][CT]GG
EaeI 131 TGGCCA [CT]^GGCC[GA]
Eco81I 633 CCTCAGG CC^T[ACGT]AGG
EcoO109I 244 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 189 CCTGG ^CC[AT]GG
EcoT14I 171 CCAAGG C^C[AT][AT]GG
EcoT22I 532 ATGCAT ATGCA^T
FbaI 395 TGATCA T^GATCA
HapII 368 CCGG C^CGG
Hin1I 542 GACGTC G[GA]^CG[CT]C
HindIII 467 AAGCTT A^AGCTT
HinfI 556 GAGTC G^A[ACGT]TC
HpaII 368 CCGG C^CGG
MaeI 4 CTAG C^TAG
MaeIII 502 GTCAC ^GT[ACGT]AC
MluNI 131 TGGCCA TGG^CCA
MscI 131 TGGCCA TGG^CCA
MseI 241 TTAA T^TAA
MspI 368 CCGG C^CGG
MvaI 189 CCTGG CC^[AT]GG
MwoI 106 GCAGTCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 367 GCCGGC GCC^GGC
NdeI 116 CATATG CA^TATG
NgoMIV 367 GCCGGC G^CCGGC
NsiI 532 ATGCAT ATGCA^T
PleI 556 GAGTC GAGTC(4/5)
PpuMI 244 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 495 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 449 CTGCAG CTGCA^G
ScrFI 189 CCTGG CC^[ACGT]GG
SexAI 188 ACCTGGT A^CC[AT]GGT
SfaNI 54 GATGC GCATC(5/9)
SmlI 240 CTTAAG C^T[CT][GA]AG
SspBI 356 TGTACA T^GTACA
StyI 171 CCAAGG C^C[AT][AT]GG
Tru9I 241 TTAA T^TAA
Tsp45I 502 GTCAC ^GT[GC]AC
Tsp509I 10 AATT ^AATT
XmnI 440 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 336 GTAC GT^AC
357 GTAC
AluI 347 AGCT AG^CT
468 AGCT
AvaII 81 GGTCC G^G[AT]CC
245 GGACC
BsaJI 171 CCAAGG C^C[ACGT][ACGT]GG
560 CCGTGG
BsmAI 540 GAGAC GTCTC(1/5)
387 GTCTC
BspLU11I 413 ACATGT A^CATGT
478 ACATGT
BsrDI 72 CATTGC GCAATG(2/0)
146 CATTGC
BsrI 318 ACTGG ACTGG(1/-1)
384 CCAGT
Cfr13I 81 GGTCC G^G[ACGT]CC
245 GGACC
EarI 389 CTCTTC CTCTTC(1/4)
523 CTCTTC
HaeIII 132 GGCC GG^CC
631 GGCC
Ksp632I 389 CTCTTC CTCTTC(1/4)
523 CTCTTC
MslI 72 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
594 CACCACAGTG
NlaIV 484 GGTTCC GG[ACGT]^[ACGT]CC
517 GGAACC
RsaI 336 GTAC GT^AC
357 GTAC
Sau96I 81 GGTCC G^G[ACGT]CC
245 GGACC
SphI 322 GCATGC GCATG^C
530 GCATGC
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AflIII BsmFI BstF5I Cac8I DdeI
DpnII FokI MaeII MboI MboII
MnlI NdeII NlaIII NspI Sau3AI
SfcI TspRI