Restriction map for
pF1KE0839
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaHI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HapII HgaI Hin1I
HpaII KasI KpnI KspI MaeII
MfeI MflI MluI MroI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AlwNI 162 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 772 CCTCAGG CC^T[ACGT]AGG
Aor51HI 760 AGCGCT AGC^GCT
AvaII 407 GGTCC G^G[AT]CC
BalI 152 TGGCCA TGG^CCA
BbvI 207 GCTGC GCAGC(8/12)
BglI 688 GCCATAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 159 CTCCAG CTGGAG(16/14)
BsaBI 129 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 659 GAGACC GGTCTC(1/5)
BsaJI 552 CCTGGG C^C[ACGT][ACGT]GG
BspHI 480 TCATGA T^CATGA
BstF5I 138 CATCC GGATG(2/0)
Bsu36I 772 CCTCAGG CC^T[ACGT]AGG
CfoI 761 GCGC GCG^C
DraII 406 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 152 TGGCCA [CT]^GGCC[GA]
EarI 113 GAAGAG CTCTTC(1/4)
Eco47III 760 AGCGCT AGC^GCT
Eco81I 772 CCTCAGG CC^T[ACGT]AGG
EcoO109I 406 GGGTCCT [GA]G^G[ACGT]CC[CT]
Fnu4HI 207 GCTGC GC^[ACGT]GC
FokI 138 CATCC GGATG(9/13)
HaeII 760 AGCGCT [GA]GCGC^[CT]
HhaI 761 GCGC GCG^C
HinP1I 761 GCGC G^CGC
HindIII 872 AAGCTT A^AGCTT
ItaI 207 GCTGC GC^[ACGT]GC
Ksp632I 113 GAAGAG CTCTTC(1/4)
MamI 129 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 152 TGGCCA TGG^CCA
MscI 152 TGGCCA TGG^CCA
NdeI 224 CATATG CA^TATG
NspI 642 GCATGC [GA]CATG^[CT]
PpuMI 406 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 480 TCATGA T^CATGA
SfcI 647 CTACAG C^T[GA][CT]AG
SmlI 325 CTCAAG C^T[CT][GA]AG
SpeI 84 ACTAGT A^CTAGT
SphI 642 GCATGC GCATG^C
TaqI 636 TCGA T^CGA
TseI 207 GCTGC G^C[AT]GC
Tsp45I 800 GTGAC ^GT[GC]AC
TspRI 698 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 636 TCGA T^CGA
XcmI 349 CCATTTTTCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
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AcsI 502 AAATTC [GA]^AATT[CT]
518 AAATTC
ApoI 502 AAATTC [GA]^AATT[CT]
518 AAATTC
Asp700I 871 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
900 GAAGTGTTTC
BclI 143 TGATCA T^GATCA
701 TGATCA
BseRI 715 CTCCTC GAGGAG(10/8)
718 CTCCTC
BsmAI 659 GAGAC GTCTC(1/5)
287 GTCTC
FbaI 143 TGATCA T^GATCA
701 TGATCA
HincII 88 GTTAAC GT[CT]^[GA]AC
540 GTTAAC
HindII 88 GTTAAC GT[CT]^[GA]AC
540 GTTAAC
HpaI 88 GTTAAC GTT^AAC
540 GTTAAC
MwoI 10 GCAATAGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
688 GCCATAAAGGC
NlaIV 406 GGGTCC GG[ACGT]^[ACGT]CC
564 GGTTCC
XmnI 871 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
900 GAAGTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI BfaI BsiYI BslI
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII HaeIII HinfI
HphI MaeI MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
RsaI Sau3AI Sau96I ScrFI TfiI
Tru9I Tsp509I