Restriction map for pF1KE0863
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# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     AspEI       AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BfrI        BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DrdI        Eam1105I    Eco47III    
Eco81I      EcoRV       EcoT22I     FseI        FspI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MseI        MspA1I      MunI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        TaqI        Tru9I       
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   62 enzymes) #
###################################

Acc65I        439  GGTACC               G^GTACC
AccI          100  GTCTAC               GT^[AC][GT]AC
AccII         817  CGCG                 CG^CG
AcsI           31  GAATTC               [GA]^AATT[CT]
AluI          209  AGCT                 AG^CT
AlwI          627  GATCC                GGATC(4/5)
ApoI           31  GAATTC               [GA]^AATT[CT]
Asp718I       439  GGTACC               G^GTACC
AspHI         208  GAGCTC               G[AT]GC[AT]^C
AspI          814  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         239  GGACC                G^G[AT]CC
BclI          131  TGATCA               T^GATCA
BfaI          679  CTAG                 C^TAG
BglI          421  GCCTGCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         590  AGATCT               A^GATCT
BsaI          744  GGTCTC               GGTCTC(1/5)
BsiEI         784  CGGCCG               CG[GA][CT]^CG
BsiHKAI       208  GAGCTC               G[AT]GC[AT]^C
BspMI         721  ACCTGC               ACCTGC(4/8)
BsrDI         682  GCAATG               GCAATG(2/0)
BsrI          550  CCAGT                ACTGG(1/-1)
BssSI         739  CTCGTG               CACGAG(-5/-1)
BstEII        108  GGTCACC              G^GT[ACGT]ACC
BstPI         108  GGTCACC              G^GT[ACGT]ACC
BstUI         817  CGCG                 CG^CG
BstXI         236  CCAGGACCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         590  AGATCT               [GA]^GATC[CT]
DraIII        910  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          784  CGGCCG               C^GGCCG
EclXI         784  CGGCCG               C^GGCCG
Eco52I        784  CGGCCG               C^GGCCG
EcoNI         141  CCTCACACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       108  GGTCACC              G^GT[ACGT]ACC
EcoRI          31  GAATTC               G^AATTC
FauI          525  CCCGC                CCCGC(4/6)
FbaI          131  TGATCA               T^GATCA
HaeII         926  AGCGCC               [GA]GCGC^[CT]
HapII         783  CCGG                 C^CGG
HgaI          818  GCGTC                GACGC(5/10)
HinfI          47  GATTC                G^A[ACGT]TC
HpaII         783  CCGG                 C^CGG
KpnI          439  GGTACC               GGTAC^C
MaeI          679  CTAG                 C^TAG
MflI          590  AGATCT               [GA]^GATC[CT]
MspI          783  CCGG                 C^CGG
MvnI          817  CGCG                 CG^CG
PflFI         814  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         238  AGGACCC              [GA]G^G[AT]CC[CT]
PstI           67  CTGCAG               CTGCA^G
SacI          208  GAGCTC               GAGCT^C
SapI          210  GCTCTTC              GCTCTTC(1/4)
SbfI           66  CCTGCAGG             CCTGCA^GG
ScaI          552  AGTACT               AGT^ACT
SexAI         104  ACCTGGT              A^CC[AT]GGT
SfcI           67  CTGCAG               C^T[GA][CT]AG
SfiI          420  GGCCTGCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I       66  CCTGCAGG             CCTGCA^GG
TfiI           47  GATTC                G^A[AT]TC
Tsp509I        32  AATT                 ^AATT
Tth111I       814  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          674  CCATCCTAGCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         590  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

ApaI           72  GGGCCC               GGGCC^C
              899  GGGCCC              
BalI          245  TGGCCA               TGG^CCA
              428  TGGCCA              
BanI          439  GGTACC               G^G[CT][GA]CC
              670  GGTGCC              
BsgI          163  CTGCAC               GTGCAG(16/14)
              293  GTGCAG              
BsmAI         745  GTCTC                GTCTC(1/5)
              827  GTCTC               
CfoI          189  GCGC                 GCG^C
              927  GCGC                
EcoT14I       353  CCATGG               C^C[AT][AT]GG
              431  CCATGG              
HhaI          189  GCGC                 GCG^C
              927  GCGC                
HinP1I        189  GCGC                 G^CGC
              927  GCGC                
MaeII         308  ACGT                 A^CGT
              890  ACGT                
MluNI         245  TGGCCA               TGG^CCA
              428  TGGCCA              
MscI          245  TGGCCA               TGG^CCA
              428  TGGCCA              
MwoI          421  GCCTGCATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              673  GCCATCCTAGC         
NcoI          353  CCATGG               C^CATGG
              431  CCATGG              
SmlI           58  CTCAAG               C^T[CT][GA]AG
              408  CTTGAG              
StyI          353  CCATGG               C^C[AT][AT]GG
              431  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AfaI        BanII       BbsI        BbvI        
BmyI        BpuAI       BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MslI        MvaI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TseI        Tsp45I      TspRI       
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