################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoRV EcoT22I FseI FspI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MroI MseI MspA1I MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI Tru9I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 439 GGTACC G^GTACC AccI 100 GTCTAC GT^[AC][GT]AC AccII 817 CGCG CG^CG AcsI 31 GAATTC [GA]^AATT[CT] AluI 209 AGCT AG^CT AlwI 627 GATCC GGATC(4/5) ApoI 31 GAATTC [GA]^AATT[CT] Asp718I 439 GGTACC G^GTACC AspHI 208 GAGCTC G[AT]GC[AT]^C AspI 814 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 239 GGACC G^G[AT]CC BclI 131 TGATCA T^GATCA BfaI 679 CTAG C^TAG BglI 421 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 590 AGATCT A^GATCT BsaI 744 GGTCTC GGTCTC(1/5) BsiEI 784 CGGCCG CG[GA][CT]^CG BsiHKAI 208 GAGCTC G[AT]GC[AT]^C BspMI 721 ACCTGC ACCTGC(4/8) BsrDI 682 GCAATG GCAATG(2/0) BsrI 550 CCAGT ACTGG(1/-1) BssSI 739 CTCGTG CACGAG(-5/-1) BstEII 108 GGTCACC G^GT[ACGT]ACC BstPI 108 GGTCACC G^GT[ACGT]ACC BstUI 817 CGCG CG^CG BstXI 236 CCAGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 590 AGATCT [GA]^GATC[CT] DraIII 910 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 784 CGGCCG C^GGCCG EclXI 784 CGGCCG C^GGCCG Eco52I 784 CGGCCG C^GGCCG EcoNI 141 CCTCACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 108 GGTCACC G^GT[ACGT]ACC EcoRI 31 GAATTC G^AATTC FauI 525 CCCGC CCCGC(4/6) FbaI 131 TGATCA T^GATCA HaeII 926 AGCGCC [GA]GCGC^[CT] HapII 783 CCGG C^CGG HgaI 818 GCGTC GACGC(5/10) HinfI 47 GATTC G^A[ACGT]TC HpaII 783 CCGG C^CGG KpnI 439 GGTACC GGTAC^C MaeI 679 CTAG C^TAG MflI 590 AGATCT [GA]^GATC[CT] MspI 783 CCGG C^CGG MvnI 817 CGCG CG^CG PflFI 814 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 238 AGGACCC [GA]G^G[AT]CC[CT] PstI 67 CTGCAG CTGCA^G SacI 208 GAGCTC GAGCT^C SapI 210 GCTCTTC GCTCTTC(1/4) SbfI 66 CCTGCAGG CCTGCA^GG ScaI 552 AGTACT AGT^ACT SexAI 104 ACCTGGT A^CC[AT]GGT SfcI 67 CTGCAG C^T[GA][CT]AG SfiI 420 GGCCTGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 66 CCTGCAGG CCTGCA^GG TfiI 47 GATTC G^A[AT]TC Tsp509I 32 AATT ^AATT Tth111I 814 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 674 CCATCCTAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 590 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### ApaI 72 GGGCCC GGGCC^C 899 GGGCCC BalI 245 TGGCCA TGG^CCA 428 TGGCCA BanI 439 GGTACC G^G[CT][GA]CC 670 GGTGCC BsgI 163 CTGCAC GTGCAG(16/14) 293 GTGCAG BsmAI 745 GTCTC GTCTC(1/5) 827 GTCTC CfoI 189 GCGC GCG^C 927 GCGC EcoT14I 353 CCATGG C^C[AT][AT]GG 431 CCATGG HhaI 189 GCGC GCG^C 927 GCGC HinP1I 189 GCGC G^CGC 927 GCGC MaeII 308 ACGT A^CGT 890 ACGT MluNI 245 TGGCCA TGG^CCA 428 TGGCCA MscI 245 TGGCCA TGG^CCA 428 TGGCCA MwoI 421 GCCTGCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 673 GCCATCCTAGC NcoI 353 CCATGG C^CATGG 431 CCATGG SmlI 58 CTCAAG C^T[CT][GA]AG 408 CTTGAG StyI 353 CCATGG C^C[AT][AT]GG 431 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AfaI BanII BbsI BbvI BmyI BpuAI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MslI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI