Restriction map for
pF1KE0875
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AvaII
AviII AvrII BamHI BanII BbeI
BbvCI BcgI BcnI BfrI BglI
BlnI BpmI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoT22I FauI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MroI MunI MvnI MwoI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 847 GGTACC G^GTACC
Asp718I 847 GGTACC G^GTACC
AspHI 810 GTGCTC G[AT]GC[AT]^C
BalI 461 TGGCCA TGG^CCA
BanI 847 GGTACC G^G[CT][GA]CC
BbrPI 807 CACGTG CAC^GTG
BbsI 788 GAAGAC GAAGAC(2/6)
BbvI 1003 GCTGC GCAGC(8/12)
BciVI 145 GGATAC GTATCC(6/5)
BclI 833 TGATCA T^GATCA
BglII 58 AGATCT A^GATCT
BlpI 812 GCTCAGC GC^T[ACGT]AGC
BmyI 810 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 812 GCTCAGC GC^T[ACGT]AGC
BpuAI 788 GAAGAC GAAGAC(2/6)
BsaAI 807 CACGTG [CT]AC^GT[GA]
BsaWI 541 TCCGGT [AT]^CCGG[AT]
BseRI 315 CTCCTC GAGGAG(10/8)
BsiHKAI 810 GTGCTC G[AT]GC[AT]^C
Bsp1286I 810 GTGCTC G[AGT]GC[ACT]^C
BstYI 58 AGATCT [GA]^GATC[CT]
CelII 812 GCTCAGC GC^T[ACGT]AGC
DrdI 108 GACACTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 683 CCATGG C^C[GA][CT]GG
EaeI 461 TGGCCA [CT]^GGCC[GA]
EcoNI 495 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 774 GAATTC G^AATTC
EcoT14I 683 CCATGG C^C[AT][AT]GG
FbaI 833 TGATCA T^GATCA
Fnu4HI 1003 GCTGC GC^[ACGT]GC
HapII 542 CCGG C^CGG
HgaI 791 GACGC GACGC(5/10)
HincII 414 GTCAAC GT[CT]^[GA]AC
HindII 414 GTCAAC GT[CT]^[GA]AC
HpaII 542 CCGG C^CGG
ItaI 1003 GCTGC GC^[ACGT]GC
KpnI 847 GGTACC GGTAC^C
MaeII 808 ACGT A^CGT
MaeIII 949 GTGAC ^GT[ACGT]AC
MflI 58 AGATCT [GA]^GATC[CT]
MluNI 461 TGGCCA TGG^CCA
MscI 461 TGGCCA TGG^CCA
MspA1I 557 CAGCTG C[AC]G^C[GT]G
MspI 542 CCGG C^CGG
NcoI 683 CCATGG C^CATGG
NlaIV 847 GGTACC GG[ACGT]^[ACGT]CC
NspI 622 GCATGT [GA]CATG^[CT]
PmaCI 807 CACGTG CAC^GTG
PmlI 807 CACGTG CAC^GTG
PvuII 557 CAGCTG CAG^CTG
SfaNI 236 GCATC GCATC(5/9)
SfcI 975 CTATAG C^T[GA][CT]AG
StyI 683 CCATGG C^C[AT][AT]GG
TseI 1003 GCTGC G^C[AT]GC
Tsp45I 949 GTGAC ^GT[GC]AC
XhoII 58 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 8 enzymes) #
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BfaI 184 CTAG C^TAG
196 CTAG
BsaJI 276 CCTGGG C^C[ACGT][ACGT]GG
683 CCATGG
BsgI 380 GTGCAG GTGCAG(16/14)
385 GTGCAG
BsmAI 582 GAGAC GTCTC(1/5)
879 GTCTC
BsrDI 212 GCAATG GCAATG(2/0)
406 GCAATG
EcoRV 298 GATATC GAT^ATC
690 GATATC
MaeI 184 CTAG C^TAG
196 CTAG
MslI 258 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
681 CACCATGGTG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BsiYI BslI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII FokI
HaeIII HinfI HphI MboI MboII
MnlI MseI MvaI NdeII NlaIII
RsaI Sau3AI ScrFI SmlI SspI
TfiI Tru9I Tsp509I TspRI XmnI