Restriction map for
pF1KE0883
##################################
# NO CUT ENZYMES ( 163 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AflIII AgeI AlwI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcnI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstYI BstZ17I CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRII EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI HinP1I HindIII HpaI
HpaII KasI KpnI KspI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MspI MunI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII Sau96I SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp45I Tth111I XbaI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 571 GACGTC GACGT^C
AccI 222 GTCGAC GT^[AC][GT]AC
AciI 398 CCGC CCGC(-3/-1)
AcsI 31 GAATTC [GA]^AATT[CT]
AcyI 571 GACGTC G[GA]^CG[CT]C
AfaI 177 GTAC GT^AC
AhdI 811 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 921 GTGCAC G^TGCAC
AocI 873 CCTAAGG CC^T[ACGT]AGG
ApaLI 921 GTGCAC G^TGCAC
ApoI 31 GAATTC [GA]^AATT[CT]
Asp700I 855 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 811 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 262 GGCACC G^G[CT][GA]CC
BcgI 758 GCACAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 638 GTATCC GTATCC(6/5)
BclI 590 TGATCA T^GATCA
BlpI 56 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 56 GCTCAGC GC^T[ACGT]AGC
BsaBI 538 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 571 GACGTC G[GA]^CG[CT]C
BsaJI 116 CCTTGG C^C[ACGT][ACGT]GG
BsmAI 574 GTCTC GTCTC(1/5)
BsmBI 573 CGTCTC CGTCTC(1/5)
BspMI 718 ACCTGC ACCTGC(4/8)
BsrDI 135 CATTGC GCAATG(2/0)
BsrI 259 ACTGG ACTGG(1/-1)
BstXI 110 CCACCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 873 CCTAAGG CC^T[ACGT]AGG
CelII 56 GCTCAGC GC^T[ACGT]AGC
Eam1105I 811 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 873 CCTAAGG CC^T[ACGT]AGG
EcoRI 31 GAATTC G^AATTC
EcoT14I 116 CCTTGG C^C[AT][AT]GG
FbaI 590 TGATCA T^GATCA
HaeIII 198 GGCC GG^CC
Hin1I 571 GACGTC G[GA]^CG[CT]C
MamI 538 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 125 ACATGC [GA]CATG^[CT]
PflMI 426 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 154 GACTC GAGTC(4/5)
PshAI 225 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 177 GTAC GT^AC
SalI 222 GTCGAC G^TCGAC
SapI 300 GCTCTTC GCTCTTC(1/4)
SfaNI 849 GATGC GCATC(5/9)
SmlI 916 CTCAAG C^T[CT][GA]AG
StyI 116 CCTTGG C^C[AT][AT]GG
TaqI 223 TCGA T^CGA
TfiI 855 GAATC G^A[AT]TC
Tsp509I 32 AATT ^AATT
TthHB8I 223 TCGA T^CGA
Van91I 426 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 426 CCAGCTAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 855 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 85 GTGCTC G[AT]GC[AT]^C
921 GTGCAC
BfaI 53 CTAG C^TAG
430 CTAG
BmyI 85 GTGCTC G[AGT]GC[ACT]^C
921 GTGCAC
BsiHKAI 85 GTGCTC G[AT]GC[AT]^C
921 GTGCAC
Bsp1286I 85 GTGCTC G[AGT]GC[ACT]^C
921 GTGCAC
BstF5I 519 CATCC GGATG(2/0)
788 CATCC
Cac8I 283 GCAGGC GC[ACGT]^[ACGT]GC
698 GCAAGC
EarI 301 CTCTTC CTCTTC(1/4)
891 GAAGAG
FokI 519 CATCC GGATG(9/13)
788 CATCC
HinfI 154 GACTC G^A[ACGT]TC
855 GAATC
Ksp632I 301 CTCTTC CTCTTC(1/4)
891 GAAGAG
MaeI 53 CTAG C^TAG
430 CTAG
MaeII 220 ACGT A^CGT
572 ACGT
MaeIII 191 GTAAC ^GT[ACGT]AC
451 GTTAC
MspA1I 493 CAGCTG C[AC]G^C[GT]G
723 CAGCTG
NdeI 356 CATATG CA^TATG
796 CATATG
NlaIII 126 CATG CATG^
828 CATG
NlaIV 262 GGCACC GG[ACGT]^[ACGT]CC
843 GGCTCC
PstI 553 CTGCAG CTGCA^G
720 CTGCAG
PvuII 493 CAGCTG CAG^CTG
723 CAGCTG
TspRI 669 CACTG CA[GC]TG[ACGT][ACGT]^
737 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI BbvI BsiYI BslI BsmFI
DdeI DpnII Fnu4HI HincII HindII
HphI ItaI MboI MboII MnlI
NdeII Sau3AI SfcI TseI