Restriction map for
pF1KE0888
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRV FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeII
MfeI MluI MroI MspA1I MunI
MvnI NarI NciI NdeI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 31 GAATTC [GA]^AATT[CT]
ApoI 31 GAATTC [GA]^AATT[CT]
Asp700I 705 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 408 GTGCTC G[AT]GC[AT]^C
AvaI 692 CTCGGG C^[CT]CG[GA]G
AvrII 937 CCTAGG C^CTAGG
BanI 619 GGCACC G^G[CT][GA]CC
BclI 449 TGATCA T^GATCA
BlnI 937 CCTAGG C^CTAGG
BsaBI 958 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 360 CGACCG CG[GA][CT]^CG
BsiHKAI 408 GTGCTC G[AT]GC[AT]^C
BsoBI 692 CTCGGG C^[CT]CG[GA]G
BspMI 478 ACCTGC ACCTGC(4/8)
BsrFI 508 GCCGGC [GA]^CCGG[CT]
BsrI 462 CCAGT ACTGG(1/-1)
BstEII 108 GGTCACC G^GT[ACGT]ACC
BstPI 108 GGTCACC G^GT[ACGT]ACC
BstYI 680 AGATCC [GA]^GATC[CT]
CfoI 506 GCGC GCG^C
Cfr10I 508 GCCGGC [GA]^CCGG[CT]
DsaI 326 CCATGG C^C[GA][CT]GG
EcoNI 932 CCTGTCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 108 GGTCACC G^GT[ACGT]ACC
EcoRI 31 GAATTC G^AATTC
EcoT22I 1006 ATGCAT ATGCA^T
FauI 384 CCCGC CCCGC(4/6)
FbaI 449 TGATCA T^GATCA
HhaI 506 GCGC GCG^C
HinP1I 506 GCGC G^CGC
HincII 205 GTTGAC GT[CT]^[GA]AC
HindII 205 GTTGAC GT[CT]^[GA]AC
MamI 958 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 680 AGATCC [GA]^GATC[CT]
MseI 677 TTAA T^TAA
MwoI 346 GCAGTAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 508 GCCGGC GCC^GGC
NcoI 326 CCATGG C^CATGG
NgoMIV 508 GCCGGC G^CCGGC
NheI 243 GCTAGC G^CTAGC
NsiI 1006 ATGCAT ATGCA^T
SexAI 104 ACCTGGT A^CC[AT]GGT
SfcI 867 CTACAG C^T[GA][CT]AG
TaqI 359 TCGA T^CGA
Tru9I 677 TTAA T^TAA
Tsp509I 32 AATT ^AATT
TthHB8I 359 TCGA T^CGA
XcmI 272 CCATCCCCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 680 AGATCC [GA]^GATC[CT]
XmnI 705 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 681 GATCC GGATC(4/5)
976 GGATC
AvaII 323 GGACC G^G[AT]CC
758 GGACC
BmyI 408 GTGCTC G[AGT]GC[ACT]^C
618 GGGCAC
BsmAI 1055 GAGAC GTCTC(1/5)
465 GTCTC
Bsp1286I 408 GTGCTC G[AGT]GC[ACT]^C
618 GGGCAC
BstXI 98 CCACATACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
581 CCAACGAGCTGG
Cac8I 243 GCTAGC GC[ACGT]^[ACGT]GC
508 GCCGGC
Cfr13I 323 GGACC G^G[ACGT]CC
758 GGACC
EarI 79 CTCTTC CTCTTC(1/4)
187 CTCTTC
EcoT14I 326 CCATGG C^C[AT][AT]GG
937 CCTAGG
HapII 509 CCGG C^CGG
563 CCGG
HpaII 509 CCGG C^CGG
563 CCGG
Ksp632I 79 CTCTTC CTCTTC(1/4)
187 CTCTTC
MspI 509 CCGG C^CGG
563 CCGG
Sau96I 323 GGACC G^G[ACGT]CC
758 GGACC
StyI 326 CCATGG C^C[AT][AT]GG
937 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI BalI BbvI
BfaI BpmI BsaJI BseRI BsiYI
BslI BsmFI BssKI BstF5I BstNI
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MslI MvaI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScrFI SfaNI TseI Tsp45I TspRI