Restriction map for
pF1KB7530
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
HindIII HpaI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MseI MspA1I MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcsI 79 GAATTT [GA]^AATT[CT]
AcyI 281 GGCGCC G[GA]^CG[CT]C
AocI 369 CCTCAGG CC^T[ACGT]AGG
ApoI 79 GAATTT [GA]^AATT[CT]
AspHI 157 GAGCAC G[AT]GC[AT]^C
AvaI 70 CCCGAG C^[CT]CG[GA]G
BanI 281 GGCGCC G^G[CT][GA]CC
BbeI 281 GGCGCC GGCGC^C
BbrPI 160 CACGTG CAC^GTG
BcnI 445 CCGGG CC^[GC]GG
BfaI 737 CTAG C^TAG
BsaAI 160 CACGTG [CT]AC^GT[GA]
BsaHI 281 GGCGCC G[GA]^CG[CT]C
BsaWI 635 ACCGGA [AT]^CCGG[AT]
BseRI 645 CTCCTC GAGGAG(10/8)
BsiHKAI 157 GAGCAC G[AT]GC[AT]^C
BsmI 375 GAATGC GAATGC(1/-1)
BsoBI 70 CCCGAG C^[CT]CG[GA]G
BspMI 420 GCAGGT ACCTGC(4/8)
BsrBI 274 GAGCGG CCGCTC(-3/-3)
BsrFI 175 ACCGGC [GA]^CCGG[CT]
BstBI 307 TTCGAA TT^CGAA
Bsu36I 369 CCTCAGG CC^T[ACGT]AGG
Cfr10I 175 ACCGGC [GA]^CCGG[CT]
DsaI 242 CCATGG C^C[GA][CT]GG
EaeI 177 CGGCCA [CT]^GGCC[GA]
Eco81I 369 CCTCAGG CC^T[ACGT]AGG
Hin1I 281 GGCGCC G[GA]^CG[CT]C
HincII 295 GTCAAC GT[CT]^[GA]AC
HindII 295 GTCAAC GT[CT]^[GA]AC
HinfI 709 GATTC G^A[ACGT]TC
KasI 281 GGCGCC G^GCGCC
MaeI 737 CTAG C^TAG
MboII 288 GAAGA GAAGA(8/7)
MslI 237 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 281 GGCGCC GG^CGCC
NciI 445 CCGGG CC^[GC]GG
NcoI 242 CCATGG C^CATGG
NspI 497 GCATGC [GA]CATG^[CT]
NspV 307 TTCGAA TT^CGAA
PflMI 672 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 160 CACGTG CAC^GTG
PmlI 160 CACGTG CAC^GTG
SbfI 399 CCTGCAGG CCTGCA^GG
SfaNI 551 GCATC GCATC(5/9)
SfoI 281 GGCGCC GGC^GCC
SfuI 307 TTCGAA TT^CGAA
SmlI 316 CTCAAG C^T[CT][GA]AG
SphI 497 GCATGC GCATG^C
Sse8387I 399 CCTGCAGG CCTGCA^GG
TfiI 709 GATTC G^A[AT]TC
Tsp45I 194 GTCAC ^GT[GC]AC
Tsp509I 80 AATT ^AATT
TspRI 196 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 672 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 180 CCACCACCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 101 CGCG CG^CG
271 CGCG
AlwNI 346 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
673 CAGGATCTG
AvaII 524 GGTCC G^G[AT]CC
633 GGACC
BanII 141 GGGCTC G[GA]GC[CT]^C
463 GAGCCC
BsrI 21 CCAGT ACTGG(1/-1)
694 CCAGT
BstAPI 215 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
402 GCAGGAGTTGC
BstF5I 381 CATCC GGATG(2/0)
12 GGATG
BstUI 101 CGCG CG^CG
271 CGCG
BstYI 365 AGATCC [GA]^GATC[CT]
675 GGATCT
EcoNI 221 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
285 CCTGAAGAAGG
EcoT14I 242 CCATGG C^C[AT][AT]GG
356 CCAAGG
FokI 381 CATCC GGATG(9/13)
12 GGATG
HaeII 168 AGCGCC [GA]GCGC^[CT]
281 GGCGCC
HaeIII 178 GGCC GG^CC
210 GGCC
MaeII 5 ACGT A^CGT
161 ACGT
MaeIII 194 GTCAC ^GT[ACGT]AC
509 GTAAC
MflI 365 AGATCC [GA]^GATC[CT]
675 GGATCT
MvnI 101 CGCG CG^CG
271 CGCG
PstI 136 CTGCAG CTGCA^G
400 CTGCAG
ScaI 38 AGTACT AGT^ACT
538 AGTACT
StyI 242 CCATGG C^C[AT][AT]GG
356 CCAAGG
TaqI 308 TCGA T^CGA
392 TCGA
TthHB8I 308 TCGA T^CGA
392 TCGA
XhoII 365 AGATCC [GA]^GATC[CT]
675 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI HapII HhaI HinP1I
HpaII HphI ItaI MboI MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfcI TseI