Restriction map for
pF1KB6831
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DdeI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MaeI MaeIII MamI MfeI MflI
MluI MseI MunI MwoI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp45I Tsp509I TspRI
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 12 TCCGGA T^CCGGA
AcyI 77 GACGCC G[GA]^CG[CT]C
AlwI 416 GGATC GGATC(4/5)
AspHI 463 GAGCTC G[AT]GC[AT]^C
AspI 43 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 434 TGGCCA TGG^CCA
BanI 381 GGTGCC G^G[CT][GA]CC
BanII 463 GAGCTC G[GA]GC[CT]^C
BbsI 146 GAAGAC GAAGAC(2/6)
BclI 290 TGATCA T^GATCA
BpuAI 146 GAAGAC GAAGAC(2/6)
BsaHI 77 GACGCC G[GA]^CG[CT]C
BsaWI 12 TCCGGA [AT]^CCGG[AT]
BseAI 12 TCCGGA T^CCGGA
BsiHKAI 463 GAGCTC G[AT]GC[AT]^C
BsmBI 75 GAGACG CGTCTC(1/5)
BspEI 12 TCCGGA T^CCGGA
BspHI 455 TCATGA T^CATGA
BstXI 152 CCAGGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 434 TGGCCA [CT]^GGCC[GA]
EcoNI 348 CCTCAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 113 CCTTGG C^C[AT][AT]GG
FbaI 290 TGATCA T^GATCA
HaeII 305 AGCGCC [GA]GCGC^[CT]
HgaI 77 GACGC GACGC(5/10)
Hin1I 77 GACGCC G[GA]^CG[CT]C
MaeII 388 ACGT A^CGT
MluNI 434 TGGCCA TGG^CCA
MroI 12 TCCGGA T^CCGGA
MscI 434 TGGCCA TGG^CCA
MslI 30 CACTACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 367 CAGCTG C[AC]G^C[GT]G
NlaIII 456 CATG CATG^
PflFI 43 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 367 CAGCTG CAG^CTG
RcaI 455 TCATGA T^CATGA
SacI 463 GAGCTC GAGCT^C
SmaI 103 CCCGGG CCC^GGG
StuI 356 AGGCCT AGG^CCT
StyI 113 CCTTGG C^C[AT][AT]GG
TaqI 419 TCGA T^CGA
Tth111I 43 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 419 TCGA T^CGA
XmaCI 103 CCCGGG C^CCGGG
XmaI 103 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaI 72 CCCGAG C^[CT]CG[GA]G
103 CCCGGG
BbvI 20 GCTGC GCAGC(8/12)
369 GCTGC
BmyI 382 GTGCCC G[AGT]GC[ACT]^C
463 GAGCTC
BsaI 421 GAGACC GGTCTC(1/5)
108 GGTCTC
BsoBI 72 CCCGAG C^[CT]CG[GA]G
103 CCCGGG
Bsp1286I 382 GTGCCC G[AGT]GC[ACT]^C
463 GAGCTC
DpnII 291 GATC ^GATC
417 GATC
DraII 119 GGGACCC [GA]G^G[ACGT]CC[CT]
179 GGGACCC
EcoO109I 119 GGGACCC [GA]G^G[ACGT]CC[CT]
179 GGGACCC
HphI 288 GGTGA GGTGA(8/7)
293 TCACC
MboI 291 GATC ^GATC
417 GATC
NdeII 291 GATC ^GATC
417 GATC
PpuMI 119 GGGACCC [GA]G^G[AT]CC[CT]
179 GGGACCC
Sau3AI 291 GATC ^GATC
417 GATC
TseI 20 GCTGC G^C[AT]GC
369 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AluI AvaII BcnI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BssKI BstF5I BstNI BstUI
CfoI Cfr13I EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI Ksp632I MboII MnlI
MspI MvaI MvnI NciI NlaIV
Sau96I ScrFI