Restriction map for
pF1KB6893
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FokI
FseI FspI HaeII HapII HgaI
Hin1I HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StyI SwaI
Tth111I Van91I XbaI XcmI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 412 GTCGAC GT^[AC][GT]AC
AfaI 360 GTAC GT^AC
AflIII 106 ACATGT A^C[GA][CT]GT
AlwI 277 GGATC GGATC(4/5)
Asp700I 334 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 21 GTGCTC G[AT]GC[AT]^C
AvaI 355 CTCGAG C^[CT]CG[GA]G
AvaII 170 GGACC G^G[AT]CC
BbsI 385 GAAGAC GAAGAC(2/6)
BbvI 508 GCTGC GCAGC(8/12)
BfaI 548 CTAG C^TAG
BmyI 21 GTGCTC G[AGT]GC[ACT]^C
BpuAI 385 GAAGAC GAAGAC(2/6)
BsaI 283 GGTCTC GGTCTC(1/5)
BsaJI 288 CCTGGG C^C[ACGT][ACGT]GG
BsiHKAI 21 GTGCTC G[AT]GC[AT]^C
BsmAI 284 GTCTC GTCTC(1/5)
BsmFI 180 GGGAC GGGAC(10/14)
BsoBI 355 CTCGAG C^[CT]CG[GA]G
Bsp1286I 21 GTGCTC G[AGT]GC[ACT]^C
BspLU11I 106 ACATGT A^CATGT
BsrDI 424 CATTGC GCAATG(2/0)
BsrI 325 ACTGG ACTGG(1/-1)
CfoI 455 GCGC GCG^C
Cfr13I 170 GGACC G^G[ACGT]CC
DpnII 278 GATC ^GATC
DraI 265 TTTAAA TTT^AAA
Fnu4HI 508 GCTGC GC^[ACGT]GC
HaeIII 352 GGCC GG^CC
HhaI 455 GCGC GCG^C
HinP1I 455 GCGC G^CGC
ItaI 508 GCTGC GC^[ACGT]GC
MaeI 548 CTAG C^TAG
MboI 278 GATC ^GATC
MspA1I 506 CAGCTG C[AC]G^C[GT]G
NdeII 278 GATC ^GATC
PaeR7I 355 CTCGAG C^TCGAG
PleI 415 GACTC GAGTC(4/5)
PvuII 506 CAGCTG CAG^CTG
RsaI 360 GTAC GT^AC
SalI 412 GTCGAC G^TCGAC
Sau3AI 278 GATC ^GATC
Sau96I 170 GGACC G^G[ACGT]CC
SfaNI 392 GCATC GCATC(5/9)
SmlI 355 CTCGAG C^T[CT][GA]AG
SphI 54 GCATGC GCATG^C
SspI 35 AATATT AAT^ATT
StuI 351 AGGCCT AGG^CCT
TfiI 162 GATTC G^A[AT]TC
TseI 508 GCTGC G^C[AT]GC
XhoI 355 CTCGAG C^TCGAG
XmnI 334 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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BseRI 95 CTCCTC GAGGAG(10/8)
98 CTCCTC
BsiYI 288 CCTGGGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
438 CCCAGCTATGG
BslI 288 CCTGGGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
438 CCCAGCTATGG
BsmI 156 GAATGC GAATGC(1/-1)
373 GAATGC
BssKI 288 CCTGG ^CC[ACGT]GG
309 CCTGG
BstNI 288 CCTGG CC^[AT]GG
309 CCTGG
DdeI 184 CTGAG C^T[ACGT]AG
229 CTAAG
EcoRII 288 CCTGG ^CC[AT]GG
309 CCTGG
HincII 71 GTCAAC GT[CT]^[GA]AC
412 GTCGAC
HindII 71 GTCAAC GT[CT]^[GA]AC
412 GTCGAC
HinfI 162 GATTC G^A[ACGT]TC
415 GACTC
HphI 328 GGTGA GGTGA(8/7)
470 TCACC
MaeIII 133 GTGAC ^GT[ACGT]AC
469 GTCAC
MvaI 288 CCTGG CC^[AT]GG
309 CCTGG
MwoI 392 GCATCAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
511 GCTTTGAGTGC
NspI 54 GCATGC [GA]CATG^[CT]
106 ACATGT
ScrFI 288 CCTGG CC^[ACGT]GG
309 CCTGG
Tsp45I 133 GTGAC ^GT[GC]AC
469 GTCAC
TspRI 19 CAGTG CA[GC]TG[ACGT][ACGT]^
143 CAGTG
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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AluI Cac8I MboII MnlI MseI
NlaIII TaqI Tru9I Tsp509I TthHB8I