##################################
# NO CUT ENZYMES (  221 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DpnII       
DraI        DraII       DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HaeIII      HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIII      NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
Sau96I      SbfI        ScaI        SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AluI          168  AGCT                 AG^CT
AlwNI         164  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BbvI          166  GCAGC                GCAGC(8/12)
BsaWI          25  ACCGGA               [AT]^CCGG[AT]
BssKI         204  CCTGG                ^CC[ACGT]GG
BstNI         204  CCTGG                CC^[AT]GG
Cac8I         140  GCAAGC               GC[ACGT]^[ACGT]GC
DraIII        202  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRII        204  CCTGG                ^CC[AT]GG
Fnu4HI        166  GCAGC                GC^[ACGT]GC
HapII          26  CCGG                 C^CGG
HgaI           18  GACGC                GACGC(5/10)
HpaII          26  CCGG                 C^CGG
HphI          201  TCACC                GGTGA(8/7)
ItaI          166  GCAGC                GC^[ACGT]GC
MseI            8  TTAA                 T^TAA
MspA1I        167  CAGCTG               C[AC]G^C[GT]G
MspI           26  CCGG                 C^CGG
MvaI          204  CCTGG                CC^[AT]GG
PvuII         167  CAGCTG               CAG^CTG
ScrFI         204  CCTGG                CC^[ACGT]GG
SexAI         203  ACCTGGT              A^CC[AT]GGT
Tru9I           8  TTAA                 T^TAA
TseI          166  GCAGC                G^C[AT]GC
Tsp509I         6  AATT                 ^AATT
TspRI         135  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BbsI           15  GAAGAC               GAAGAC(2/6)
              159  GAAGAC              
BpuAI          15  GAAGAC               GAAGAC(2/6)
              159  GAAGAC              
BsiYI         198  CCTTCACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              204  CCTGGTGTTGG         
BslI          198  CCTTCACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              204  CCTGGTGTTGG         
DdeI          217  CTGAG                C^T[ACGT]AG
              235  CTTAG               
HinfI          74  GAATC                G^A[ACGT]TC
              104  GAATC               
MboII          15  GAAGA                GAAGA(8/7)
              159  GAAGA               
SfcI           56  CTACAG               C^T[GA][CT]AG
              132  CTACAG              
TfiI           74  GAATC                G^A[AT]TC
              104  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

BseRI       MnlI