Restriction map for
pF1KB6872
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# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BciVI
BclI BcnI BfrI BglI BglII
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssKI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI ItaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI MspI MvaI
NaeI NarI NciI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI ScrFI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TseI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 250 CGCG CG^CG
AciI 570 CCGC CCGC(-3/-1)
AfaI 63 GTAC GT^AC
AflIII 156 ACATGT A^C[GA][CT]GT
AlwI 20 GGATC GGATC(4/5)
AseI 277 ATTAAT AT^TAAT
AsnI 277 ATTAAT AT^TAAT
AvrII 54 CCTAGG C^CTAGG
BcgI 240 GCAAGCACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 55 CTAG C^TAG
BlnI 54 CCTAGG C^CTAGG
BpmI 46 CTCCAG CTGGAG(16/14)
BsiYI 387 CCTTATCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 387 CCTTATCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 504 GAATGC GAATGC(1/-1)
Bsp1407I 62 TGTACA T^GTACA
BspLU11I 156 ACATGT A^CATGT
BsrGI 62 TGTACA T^GTACA
BstF5I 172 CATCC GGATG(2/0)
BstUI 250 CGCG CG^CG
BstYI 20 GGATCT [GA]^GATC[CT]
Cfr13I 150 GGGCC G^G[ACGT]CC
DdeI 90 CTTAG C^T[ACGT]AG
DsaI 515 CCATGG C^C[GA][CT]GG
FokI 172 CATCC GGATG(9/13)
HaeIII 151 GGCC GG^CC
HinfI 328 GAATC G^A[ACGT]TC
MaeI 55 CTAG C^TAG
MaeII 246 ACGT A^CGT
MfeI 194 CAATTG C^AATTG
MflI 20 GGATCT [GA]^GATC[CT]
MslI 516 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 570 CCGCTG C[AC]G^C[GT]G
MunI 194 CAATTG C^AATTG
MvnI 250 CGCG CG^CG
MwoI 540 GCTCAAGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 515 CCATGG C^CATGG
PshBI 277 ATTAAT AT^TAAT
RsaI 63 GTAC GT^AC
SapI 184 GAAGAGC GCTCTTC(1/4)
Sau96I 150 GGGCC G^G[ACGT]CC
SfaNI 418 GATGC GCATC(5/9)
SmlI 541 CTCAAG C^T[CT][GA]AG
SspBI 62 TGTACA T^GTACA
TfiI 328 GAATC G^A[AT]TC
Tsp45I 487 GTGAC ^GT[GC]AC
XhoII 20 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AspHI 43 GTGCTC G[AT]GC[AT]^C
538 GTGCTC
BsaJI 54 CCTAGG C^C[ACGT][ACGT]GG
515 CCATGG
BsgI 305 GTGCAG GTGCAG(16/14)
531 GTGCAG
BsiHKAI 43 GTGCTC G[AT]GC[AT]^C
538 GTGCTC
BsrI 48 CCAGT ACTGG(1/-1)
582 CCAGT
BssSI 235 CACGAG CACGAG(-5/-1)
322 CTCGTG
EarI 119 CTCTTC CTCTTC(1/4)
184 GAAGAG
EcoT14I 54 CCTAGG C^C[AT][AT]GG
515 CCATGG
Ksp632I 119 CTCTTC CTCTTC(1/4)
184 GAAGAG
NspI 128 ACATGC [GA]CATG^[CT]
156 ACATGT
StyI 54 CCTAGG C^C[AT][AT]GG
515 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BmyI Bsp1286I Cac8I DpnII
MaeIII MboI MboII MnlI MseI
NdeII NlaIII Sau3AI Tru9I Tsp509I
TspRI