Restriction map for
pF1KE0681
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AciI 23 GCGG CCGC(-3/-1)
AluI 317 AGCT AG^CT
AlwI 418 GATCC GGATC(4/5)
AlwNI 322 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 208 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 679 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 676 GAAGAC GAAGAC(2/6)
BcgI 657 CGATTACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 36 GTATCC GTATCC(6/5)
BcnI 541 CCCGG CC^[GC]GG
BmyI 665 GTGCCC G[AGT]GC[ACT]^C
BpuAI 676 GAAGAC GAAGAC(2/6)
BsmBI 695 GAGACG CGTCTC(1/5)
Bsp1286I 665 GTGCCC G[AGT]GC[ACT]^C
BstXI 567 CCAAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 14 GCGC GCG^C
DraII 637 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 490 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 188 CCACGG C^C[GA][CT]GG
EarI 330 GAAGAG CTCTTC(1/4)
EcoO109I 637 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 567 CCAAGG C^C[AT][AT]GG
HapII 542 CCGG C^CGG
HhaI 14 GCGC GCG^C
HinP1I 14 GCGC G^CGC
HpaII 542 CCGG C^CGG
Ksp632I 330 GAAGAG CTCTTC(1/4)
MaeII 716 ACGT A^CGT
MseI 806 TTAA T^TAA
MspI 542 CCGG C^CGG
MwoI 14 GCGCCCGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 541 CCCGG CC^[GC]GG
NspI 581 ACATGC [GA]CATG^[CT]
PflFI 679 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 637 AGGACCT [GA]G^G[AT]CC[CT]
SfcI 319 CTACAG C^T[GA][CT]AG
StyI 567 CCAAGG C^C[AT][AT]GG
TfiI 518 GAATC G^A[AT]TC
Tru9I 806 TTAA T^TAA
Tth111I 679 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 208 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AvaII 480 GGTCC G^G[AT]CC
638 GGACC
BsaI 510 GAGACC GGTCTC(1/5)
684 GGTCTC
BsgI 718 GTGCAG GTGCAG(16/14)
754 GTGCAG
BsiYI 90 CCATTGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
470 CCTATAAAGGG
BslI 90 CCATTGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
470 CCTATAAAGGG
BsmI 776 GAATGC GAATGC(1/-1)
621 GCATTC
BsrI 9 CCAGT ACTGG(1/-1)
593 CCAGT
Cfr13I 480 GGTCC G^G[ACGT]CC
638 GGACC
DpnII 418 GATC ^GATC
611 GATC
DrdI 591 GACCAGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
703 GACTACATCGTC
HincII 52 GTCAAC GT[CT]^[GA]AC
712 GTCAAC
HindII 52 GTCAAC GT[CT]^[GA]AC
712 GTCAAC
HphI 185 TCACC GGTGA(8/7)
625 TCACC
MaeIII 301 GTCAC ^GT[ACGT]AC
424 GTGAC
MboI 418 GATC ^GATC
611 GATC
MslI 186 CACCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
447 CACCCAGGTG
NdeII 418 GATC ^GATC
611 GATC
NlaIV 479 GGGTCC GG[ACGT]^[ACGT]CC
544 GGAGCC
PleI 336 GACTC GAGTC(4/5)
243 GAGTC
Sau3AI 418 GATC ^GATC
611 GATC
Sau96I 480 GGTCC G^G[ACGT]CC
638 GGACC
SfaNI 463 GATGC GCATC(5/9)
296 GCATC
Tsp45I 301 GTCAC ^GT[GC]AC
424 GTGAC
Tsp509I 236 AATT ^AATT
790 AATT
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AfaI BbvI BsaJI BsmAI BssKI
BstF5I BstNI DdeI EcoRII Fnu4HI
FokI HinfI ItaI MboII MnlI
MvaI NlaIII RsaI ScrFI TseI
TspRI