Restriction map for
pF1KE0751
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AfaI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfaI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluNI MscI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tru9I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 877 TCCGGA T^CCGGA
AcsI 127 AAATTT [GA]^AATT[CT]
AflIII 623 ACGCGT A^C[GA][CT]GT
AlwNI 836 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 596 GGGCCC GGGCC^C
ApoI 127 AAATTT [GA]^AATT[CT]
BanI 22 GGCACC G^G[CT][GA]CC
BciVI 976 GGATAC GTATCC(6/5)
BlpI 553 GCTCAGC GC^T[ACGT]AGC
BpmI 180 CTCCAG CTGGAG(16/14)
Bpu1102I 553 GCTCAGC GC^T[ACGT]AGC
BsaWI 877 TCCGGA [AT]^CCGG[AT]
BseAI 877 TCCGGA T^CCGGA
BsmAI 113 GAGAC GTCTC(1/5)
BspEI 877 TCCGGA T^CCGGA
BspHI 155 TCATGA T^CATGA
BsrDI 415 GCAATG GCAATG(2/0)
BsrI 751 CCAGT ACTGG(1/-1)
BstF5I 644 GGATG GGATG(2/0)
BstXI 659 CCAACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 162 AGATCC [GA]^GATC[CT]
CelII 553 GCTCAGC GC^T[ACGT]AGC
DraII 837 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 837 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 653 GATATC GAT^ATC
FauI 344 GCGGG CCCGC(4/6)
FokI 644 GGATG GGATG(9/13)
HaeII 34 AGCGCC [GA]GCGC^[CT]
HgaI 622 GACGC GACGC(5/10)
HphI 97 TCACC GGTGA(8/7)
MaeII 202 ACGT A^CGT
MflI 162 AGATCC [GA]^GATC[CT]
MluI 623 ACGCGT A^CGCGT
MroI 877 TCCGGA T^CCGGA
NcoI 617 CCATGG C^CATGG
PflMI 703 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 837 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 155 TCATGA T^CATGA
TspRI 752 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 703 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 933 CCACGGCATAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 162 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 624 CGCG CG^CG
803 CGCG
AlwI 163 GATCC GGATC(4/5)
784 GGATC
BanII 596 GGGCCC G[GA]GC[CT]^C
918 GGGCTC
BmyI 596 GGGCCC G[AGT]GC[ACT]^C
918 GGGCTC
BsmFI 583 GGGAC GGGAC(10/14)
603 GGGAC
Bsp1286I 596 GGGCCC G[AGT]GC[ACT]^C
918 GGGCTC
BstUI 624 CGCG CG^CG
803 CGCG
EcoT14I 18 CCAAGG C^C[AT][AT]GG
617 CCATGG
MspA1I 737 CAGCGG C[AC]G^C[GT]G
761 CAGCGG
MvnI 624 CGCG CG^CG
803 CGCG
PleI 43 GACTC GAGTC(4/5)
874 GACTC
SfaNI 412 GATGC GCATC(5/9)
645 GATGC
SmaI 599 CCCGGG CCC^GGG
819 CCCGGG
SspI 333 AATATT AAT^ATT
383 AATATT
StyI 18 CCAAGG C^C[AT][AT]GG
617 CCATGG
TaqI 297 TCGA T^CGA
389 TCGA
TfiI 259 GATTC G^A[AT]TC
507 GATTC
TthHB8I 297 TCGA T^CGA
389 TCGA
XmaCI 599 CCCGGG C^CCGGG
819 CCCGGG
XmaI 599 CCCGGG C^CCGGG
819 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AvaI AvaII BbvI
BcnI BsaJI BsiYI BslI BsoBI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TseI Tsp45I
Tsp509I