Restriction map for
pF1KB7622
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI Aor51HI ApaLI AscI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BbvI BclI BcnI
BfrI BglI BglII BlnI BsaAI
BsaHI BsaI BseAI BsiEI BsiWI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CfoI ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FauI FbaI Fnu4HI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI ItaI KasI KpnI
KspI MaeII MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI TseI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 431 GTCGAC GT^[AC][GT]AC
AfaI 448 GTAC GT^AC
AgeI 417 ACCGGT A^CCGGT
AocI 555 CCTAAGG CC^T[ACGT]AGG
ApaI 193 GGGCCC GGGCC^C
AseI 967 ATTAAT AT^TAAT
AsnI 967 ATTAAT AT^TAAT
BamHI 90 GGATCC G^GATCC
BcgI 220 CGAGAACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 788 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 788 GCTCAGC GC^T[ACGT]AGC
BsaBI 340 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 417 ACCGGT [AT]^CCGG[AT]
BsgI 5 CTGCAC GTGCAG(16/14)
BsmAI 250 GAGAC GTCTC(1/5)
BsmBI 250 GAGACG CGTCTC(1/5)
BspMI 710 GCAGGT ACCTGC(4/8)
BsrFI 417 ACCGGT [GA]^CCGG[CT]
BstYI 90 GGATCC [GA]^GATC[CT]
Bsu36I 555 CCTAAGG CC^T[ACGT]AGG
Cac8I 212 GCCAGC GC[ACGT]^[ACGT]GC
CelII 788 GCTCAGC GC^T[ACGT]AGC
Cfr10I 417 ACCGGT [GA]^CCGG[CT]
DraI 679 TTTAAA TTT^AAA
DraII 193 GGGCCCC [GA]G^G[ACGT]CC[CT]
Eco81I 555 CCTAAGG CC^T[ACGT]AGG
EcoO109I 193 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 704 CCAAGG C^C[AT][AT]GG
HapII 418 CCGG C^CGG
HgaI 252 GACGC GACGC(5/10)
HincII 431 GTCGAC GT[CT]^[GA]AC
HindII 431 GTCGAC GT[CT]^[GA]AC
HpaII 418 CCGG C^CGG
HphI 187 TCACC GGTGA(8/7)
MaeIII 370 GTGAC ^GT[ACGT]AC
MamI 340 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 90 GGATCC [GA]^GATC[CT]
MspI 418 CCGG C^CGG
PflMI 349 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 417 ACCGGT A^CCGGT
PleI 896 GAGTC GAGTC(4/5)
PshBI 967 ATTAAT AT^TAAT
RsaI 448 GTAC GT^AC
SacI 544 GAGCTC GAGCT^C
SalI 431 GTCGAC G^TCGAC
ScaI 447 AGTACT AGT^ACT
SfcI 322 CTACAG C^T[GA][CT]AG
SpeI 450 ACTAGT A^CTAGT
StyI 704 CCAAGG C^C[AT][AT]GG
Tsp45I 370 GTGAC ^GT[GC]AC
Van91I 349 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 90 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 544 GAGCTC G[AT]GC[AT]^C
593 GAGCAC
BbsI 457 GAAGAC GAAGAC(2/6)
1064 GAAGAC
BciVI 175 GGATAC GTATCC(6/5)
116 GTATCC
BfaI 451 CTAG C^TAG
598 CTAG
BpuAI 457 GAAGAC GAAGAC(2/6)
1064 GAAGAC
BseRI 85 GAGGAG GAGGAG(10/8)
361 GAGGAG
BsiHKAI 544 GAGCTC G[AT]GC[AT]^C
593 GAGCAC
BsmI 1006 GAATGC GAATGC(1/-1)
859 GCATTC
BsrI 904 ACTGG ACTGG(1/-1)
988 ACTGG
Cfr13I 193 GGGCC G^G[ACGT]CC
194 GGCCC
EarI 55 GAAGAG CTCTTC(1/4)
103 GAAGAG
EcoRI 812 GAATTC G^AATTC
954 GAATTC
HaeIII 194 GGCC GG^CC
245 GGCC
Ksp632I 55 GAAGAG CTCTTC(1/4)
103 GAAGAG
MaeI 451 CTAG C^TAG
598 CTAG
MseI 680 TTAA T^TAA
968 TTAA
NlaIII 462 CATG CATG^
637 CATG
Sau96I 193 GGGCC G^G[ACGT]CC
194 GGCCC
SfaNI 508 GATGC GCATC(5/9)
535 GCATC
SmlI 470 CTCAAG C^T[CT][GA]AG
526 CTCAAG
Tru9I 680 TTAA T^TAA
968 TTAA
XcmI 180 CCAGGATTCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
636 CCATGAAGTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
BanII BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
DdeI DpnII EcoRII FokI HinfI
MboI MboII MnlI MslI MvaI
MwoI NdeII NlaIV Sau3AI ScrFI
TaqI TfiI Tsp509I TspRI TthHB8I
XmnI