Restriction map for
pF1KB6522
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BmyI BsaHI BsaJI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmFI BsmI
BsoBI Bsp1286I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FauI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
HphI KasI KpnI KspI MaeI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI SphI Sse8387I StuI StyI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 811 GTAC GT^AC
Asp700I 9 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 472 GGTCC G^G[AT]CC
BbsI 412 GAAGAC GAAGAC(2/6)
BbvCI 1057 GCTGAGG CCTCAGC(-5/-2)
BbvI 740 GCTGC GCAGC(8/12)
BclI 387 TGATCA T^GATCA
BlpI 483 GCTAAGC GC^T[ACGT]AGC
BpmI 192 CTCCAG CTGGAG(16/14)
Bpu1102I 483 GCTAAGC GC^T[ACGT]AGC
BpuAI 412 GAAGAC GAAGAC(2/6)
BsaBI 922 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 863 GAGACC GGTCTC(1/5)
BsgI 434 CTGCAC GTGCAG(16/14)
BsmBI 213 GAGACG CGTCTC(1/5)
Bsp1407I 810 TGTACA T^GTACA
BsrDI 284 CATTGC GCAATG(2/0)
BsrGI 810 TGTACA T^GTACA
BssKI 475 CCTGG ^CC[ACGT]GG
BssSI 176 CTCGTG CACGAG(-5/-1)
BstNI 475 CCTGG CC^[AT]GG
CelII 483 GCTAAGC GC^T[ACGT]AGC
DraI 84 TTTAAA TTT^AAA
EcoRII 475 CCTGG ^CC[AT]GG
FbaI 387 TGATCA T^GATCA
Fnu4HI 740 GCTGC GC^[ACGT]GC
HindIII 440 AAGCTT A^AGCTT
ItaI 740 GCTGC GC^[ACGT]GC
MaeIII 127 GTTAC ^GT[ACGT]AC
MamI 922 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 738 CAGCTG C[AC]G^C[GT]G
MvaI 475 CCTGG CC^[AT]GG
PpuMI 471 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 738 CAGCTG CAG^CTG
RsaI 811 GTAC GT^AC
ScrFI 475 CCTGG CC^[ACGT]GG
SnaBI 150 TACGTA TAC^GTA
SspBI 810 TGTACA T^GTACA
SspI 647 AATATT AAT^ATT
TseI 740 GCTGC G^C[AT]GC
XcmI 1124 CCAACCTTCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 9 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 543 CCGC CCGC(-3/-1)
774 GCGG
AlwI 90 GGATC GGATC(4/5)
708 GGATC
AlwNI 750 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
920 CAGATACTG
BsiYI 543 CCGCCCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1128 CCTTCACTTGG
BslI 543 CCGCCCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1128 CCTTCACTTGG
BstYI 90 GGATCT [GA]^GATC[CT]
708 GGATCT
Cac8I 479 GCTTGC GC[ACGT]^[ACGT]GC
736 GCCAGC
DraII 471 AGGTCCT [GA]G^G[ACGT]CC[CT]
794 AGGCCCT
EcoO109I 471 AGGTCCT [GA]G^G[ACGT]CC[CT]
794 AGGCCCT
EcoRV 218 GATATC GAT^ATC
1030 GATATC
HgaI 517 GCGTC GACGC(5/10)
697 GCGTC
MflI 90 GGATCT [GA]^GATC[CT]
708 GGATCT
MslI 558 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1072 CATTATTATG
PleI 190 GACTC GAGTC(4/5)
785 GACTC
SfaNI 27 GATGC GCATC(5/9)
752 GATGC
TfiI 886 GATTC G^A[AT]TC
1102 GAATC
XhoII 90 GGATCT [GA]^GATC[CT]
708 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AcsI AluI ApoI BsaAI BseRI
BsmAI BsrI BstF5I Cfr13I DdeI
DpnII EarI FokI HaeIII HinfI
Ksp632I MaeII MboI MboII MnlI
MseI MwoI NdeII NlaIII Sau3AI
Sau96I Tru9I Tsp509I TspRI