################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NcoI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AfaI 264 GTAC GT^AC AflIII 176 ACACGT A^C[GA][CT]GT ApaI 15 GGGCCC GGGCC^C BamHI 629 GGATCC G^GATCC BbeI 445 GGCGCC GGCGC^C BpmI 450 CTGGAG CTGGAG(16/14) BseRI 1164 CTCCTC GAGGAG(10/8) BsmAI 703 GAGAC GTCTC(1/5) BsmBI 703 GAGACG CGTCTC(1/5) BsrBI 461 CCGCTC CCGCTC(-3/-3) BssHII 283 GCGCGC G^CGCGC BssSI 499 CACGAG CACGAG(-5/-1) BstXI 677 CCACCGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 629 GGATCC [GA]^GATC[CT] DsaI 1274 CCGCGG C^C[GA][CT]GG KasI 445 GGCGCC G^GCGCC KspI 1274 CCGCGG CCGC^GG MflI 629 GGATCC [GA]^GATC[CT] MslI 678 CACCGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 243 GCCGGC GCC^GGC NarI 445 GGCGCC GG^CGCC NdeI 609 CATATG CA^TATG NgoMIV 243 GCCGGC G^CCGGC NheI 636 GCTAGC G^CTAGC NspI 824 GCATGT [GA]CATG^[CT] PvuII 1189 CAGCTG CAG^CTG RsaI 264 GTAC GT^AC SacII 1274 CCGCGG CCGC^GG SapI 201 GCTCTTC GCTCTTC(1/4) SexAI 838 ACCTGGT A^CC[AT]GGT SfoI 445 GGCGCC GGC^GCC TfiI 1135 GATTC G^A[AT]TC Tsp45I 835 GTGAC ^GT[GC]AC Tsp509I 1255 AATT ^AATT XhoII 629 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwI 630 GATCC GGATC(4/5) 629 GGATC BanII 15 GGGCCC G[GA]GC[CT]^C 1279 GAGCCC BsgI 153 CTGCAC GTGCAG(16/14) 658 GTGCAG BsiEI 89 CGGCCG CG[GA][CT]^CG 1291 CGGCCG BsrFI 243 GCCGGC [GA]^CCGG[CT] 679 ACCGGC BsrI 534 CCAGT ACTGG(1/-1) 689 CCAGT BstAPI 651 GCAGGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1101 GCAGGTGCTGC Cfr10I 243 GCCGGC [GA]^CCGG[CT] 679 ACCGGC DpnII 141 GATC ^GATC 630 GATC EagI 89 CGGCCG C^GGCCG 1291 CGGCCG EclXI 89 CGGCCG C^GGCCG 1291 CGGCCG Eco52I 89 CGGCCG C^GGCCG 1291 CGGCCG EcoNI 620 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1095 CCTACAGCAGG MboI 141 GATC ^GATC 630 GATC NdeII 141 GATC ^GATC 630 GATC NotI 88 GCGGCCGC GC^GGCCGC 1290 GCGGCCGC PpuMI 721 AGGACCC [GA]G^G[AT]CC[CT] 1217 AGGACCC Sau3AI 141 GATC ^GATC 630 GATC SmaI 66 CCCGGG CCC^GGG 1022 CCCGGG StuI 695 AGGCCT AGG^CCT 897 AGGCCT TspRI 535 CAGTG CA[GC]TG[ACGT][ACGT]^ 1055 CACTG XcmI 182 CCAACTTCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 797 CCAGCCTGACGCTGG XmaCI 66 CCCGGG C^CCGGG 1022 CCCGGG XmaI 66 CCCGGG C^CCGGG 1022 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaI AvaII AviII BalI BanI BbvI BcnI BfaI BglI BmyI BsaHI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PstI Sau96I ScrFI SfaNI SfcI SfiI TaqI TseI TthHB8I