Restriction map for
pF1KE0736
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI AlwNI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspHI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccIII 1259 TCCGGA T^CCGGA
AcyI 994 GACGCC G[GA]^CG[CT]C
AfaI 251 GTAC GT^AC
AflIII 1232 ACATGT A^C[GA][CT]GT
Alw44I 1186 GTGCAC G^TGCAC
AocI 592 CCTGAGG CC^T[ACGT]AGG
ApaI 542 GGGCCC GGGCC^C
ApaLI 1186 GTGCAC G^TGCAC
Asp700I 1062 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1186 GTGCAC G[AT]GC[AT]^C
AvaI 554 CCCGGG C^[CT]CG[GA]G
BalI 1253 TGGCCA TGG^CCA
BbsI 316 GAAGAC GAAGAC(2/6)
BlpI 359 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 359 GCTCAGC GC^T[ACGT]AGC
BpuAI 316 GAAGAC GAAGAC(2/6)
BsaHI 994 GACGCC G[GA]^CG[CT]C
BseAI 1259 TCCGGA T^CCGGA
BsiHKAI 1186 GTGCAC G[AT]GC[AT]^C
BsmBI 149 CGTCTC CGTCTC(1/5)
BsoBI 554 CCCGGG C^[CT]CG[GA]G
BspEI 1259 TCCGGA T^CCGGA
BspLU11I 1232 ACATGT A^CATGT
BspMI 1004 ACCTGC ACCTGC(4/8)
BsrBI 628 GAGCGG CCGCTC(-3/-3)
BstXI 1028 CCAGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 592 CCTGAGG CC^T[ACGT]AGG
CelII 359 GCTCAGC GC^T[ACGT]AGC
DrdI 655 GACATTAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 206 CCGTGG C^C[GA][CT]GG
EarI 813 GAAGAG CTCTTC(1/4)
Eco81I 592 CCTGAGG CC^T[ACGT]AGG
EcoNI 567 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 994 GACGCC G[GA]^CG[CT]C
HincII 652 GTTGAC GT[CT]^[GA]AC
HindII 652 GTTGAC GT[CT]^[GA]AC
Ksp632I 813 GAAGAG CTCTTC(1/4)
MaeIII 825 GTCAC ^GT[ACGT]AC
MluNI 1253 TGGCCA TGG^CCA
MroI 1259 TCCGGA T^CCGGA
MscI 1253 TGGCCA TGG^CCA
MspA1I 700 CAGCTG C[AC]G^C[GT]G
NspI 1232 ACATGT [GA]CATG^[CT]
PstI 869 CTGCAG CTGCA^G
PvuII 700 CAGCTG CAG^CTG
RsaI 251 GTAC GT^AC
SapI 813 GAAGAGC GCTCTTC(1/4)
SmaI 554 CCCGGG CCC^GGG
SmlI 1081 CTCAAG C^T[CT][GA]AG
TfiI 1097 GATTC G^A[AT]TC
Tsp45I 825 GTCAC ^GT[GC]AC
Tsp509I 1182 AATT ^AATT
XcmI 911 CCAGCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 554 CCCGGG C^CCGGG
XmaI 554 CCCGGG C^CCGGG
XmnI 1062 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 62 CGCG CG^CG
257 CGCG
AlwI 447 GATCC GGATC(4/5)
937 GATCC
AviII 263 TGCGCA TGC^GCA
1040 TGCGCA
BfaI 304 CTAG C^TAG
313 CTAG
BpmI 52 CTGGAG CTGGAG(16/14)
778 CTGGAG
BsaI 1051 GAGACC GGTCTC(1/5)
71 GGTCTC
BstUI 62 CGCG CG^CG
257 CGCG
BstYI 446 AGATCC [GA]^GATC[CT]
936 AGATCC
DpnII 447 GATC ^GATC
937 GATC
FauI 563 CCCGC CCCGC(4/6)
1158 CCCGC
FspI 263 TGCGCA TGC^GCA
1040 TGCGCA
HaeII 28 AGCGCC [GA]GCGC^[CT]
790 AGCGCC
HgaI 994 GACGC GACGC(5/10)
1172 GCGTC
MaeI 304 CTAG C^TAG
313 CTAG
MboI 447 GATC ^GATC
937 GATC
MflI 446 AGATCC [GA]^GATC[CT]
936 AGATCC
MseI 659 TTAA T^TAA
1217 TTAA
MvnI 62 CGCG CG^CG
257 CGCG
NdeII 447 GATC ^GATC
937 GATC
PpuMI 15 AGGACCC [GA]G^G[AT]CC[CT]
389 AGGTCCC
Sau3AI 447 GATC ^GATC
937 GATC
SexAI 959 ACCAGGT A^CC[AT]GGT
1034 ACCTGGT
SfaNI 138 GATGC GCATC(5/9)
94 GCATC
SfcI 36 CTGTAG C^T[GA][CT]AG
869 CTGCAG
Tru9I 659 TTAA T^TAA
1217 TTAA
XhoII 446 AGATCC [GA]^GATC[CT]
936 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboII MnlI
MslI MspI MvaI MwoI NciI
NlaIII NlaIV PleI Sau96I ScrFI
StyI TseI TspRI