Restriction map for
pF1KE0931
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BanI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 624 CCGC CCGC(-3/-1)
Asp700I 298 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 440 GAGCAC G[AT]GC[AT]^C
AspI 922 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 125 CCCGGG C^[CT]CG[GA]G
AvaII 186 GGACC G^G[AT]CC
BalI 990 TGGCCA TGG^CCA
BanII 712 GAGCCC G[GA]GC[CT]^C
BcgI 633 CGAGGAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 272 CTCCAG CTGGAG(16/14)
BsiHKAI 440 GAGCAC G[AT]GC[AT]^C
BsoBI 125 CCCGGG C^[CT]CG[GA]G
BspHI 902 TCATGA T^CATGA
BstBI 551 TTCGAA TT^CGAA
DsaI 18 CCGTGG C^C[GA][CT]GG
EaeI 990 TGGCCA [CT]^GGCC[GA]
EcoNI 987 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 580 GATATC GAT^ATC
EcoT14I 987 CCTTGG C^C[AT][AT]GG
FauI 623 CCCGC CCCGC(4/6)
HapII 126 CCGG C^CGG
HpaII 126 CCGG C^CGG
MluNI 990 TGGCCA TGG^CCA
MscI 990 TGGCCA TGG^CCA
MslI 897 CACAGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 624 CCGCTG C[AC]G^C[GT]G
MspI 126 CCGG C^CGG
NspI 618 ACATGC [GA]CATG^[CT]
NspV 551 TTCGAA TT^CGAA
PflFI 922 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 945 GACTC GAGTC(4/5)
PpuMI 185 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 945 GACTCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 827 CTGCAG CTGCA^G
RcaI 902 TCATGA T^CATGA
SexAI 362 ACCTGGT A^CC[AT]GGT
SfuI 551 TTCGAA TT^CGAA
SmaI 125 CCCGGG CCC^GGG
StuI 164 AGGCCT AGG^CCT
StyI 987 CCTTGG C^C[AT][AT]GG
Tth111I 922 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 773 CCAGACAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
XmnI 298 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AclI 390 AACGTT AA^CGTT
793 AACGTT
AfaI 79 GTAC GT^AC
892 GTAC
BcnI 125 CCCGG CC^[GC]GG
126 CCGGG
BfaI 742 CTAG C^TAG
910 CTAG
BmyI 440 GAGCAC G[AGT]GC[ACT]^C
712 GAGCCC
BsmFI 277 GGGAC GGGAC(10/14)
331 GGGAC
Bsp1286I 440 GAGCAC G[AGT]GC[ACT]^C
712 GAGCCC
BsrI 484 ACTGG ACTGG(1/-1)
558 ACTGG
BstF5I 612 CATCC GGATG(2/0)
1038 GGATG
CfoI 33 GCGC GCG^C
807 GCGC
DraII 70 AGGCCCT [GA]G^G[ACGT]CC[CT]
185 AGGACCT
DraIII 349 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
965 CACATGGTG
EarI 174 GAAGAG CTCTTC(1/4)
1108 GAAGAG
EcoO109I 70 AGGCCCT [GA]G^G[ACGT]CC[CT]
185 AGGACCT
FokI 612 CATCC GGATG(9/13)
1038 GGATG
HaeII 32 GGCGCT [GA]GCGC^[CT]
806 GGCGCT
HhaI 33 GCGC GCG^C
807 GCGC
HinP1I 33 GCGC G^CGC
807 GCGC
Ksp632I 174 GAAGAG CTCTTC(1/4)
1108 GAAGAG
MaeI 742 CTAG C^TAG
910 CTAG
MaeII 391 ACGT A^CGT
794 ACGT
MseI 463 TTAA T^TAA
659 TTAA
NciI 125 CCCGG CC^[GC]GG
126 CCGGG
Psp1406I 390 AACGTT AA^CGTT
793 AACGTT
RsaI 79 GTAC GT^AC
892 GTAC
Tru9I 463 TTAA T^TAA
659 TTAA
Tsp45I 8 GTGAC ^GT[GC]AC
232 GTGAC
Tsp509I 199 AATT ^AATT
294 AATT
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AluI AlwI AlwNI BamHI BbvI
BsaJI BseRI BsiYI BslI BssKI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
TseI TspRI TthHB8I XhoII