Restriction map for
pF1KE0937
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MaeII MamI MfeI
MluI MroI MslI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 244 GTCTAC GT^[AC][GT]AC
AccII 861 CGCG CG^CG
AcsI 97 GAATTC [GA]^AATT[CT]
Alw44I 1213 GTGCAC G^TGCAC
AlwI 1012 GGATC GGATC(4/5)
AocI 265 CCTGAGG CC^T[ACGT]AGG
ApaLI 1213 GTGCAC G^TGCAC
ApoI 97 GAATTC [GA]^AATT[CT]
AspI 373 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 60 TGGCCA TGG^CCA
BglI 846 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 917 AGATCT A^GATCT
BlpI 177 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 177 GCTCAGC GC^T[ACGT]AGC
BseRI 118 GAGGAG GAGGAG(10/8)
BsmI 1124 GCATTC GAATGC(1/-1)
BspMI 651 GCAGGT ACCTGC(4/8)
BsrBI 228 GAGCGG CCGCTC(-3/-3)
BstUI 861 CGCG CG^CG
BstYI 917 AGATCT [GA]^GATC[CT]
Bsu36I 265 CCTGAGG CC^T[ACGT]AGG
CelII 177 GCTCAGC GC^T[ACGT]AGC
Eco81I 265 CCTGAGG CC^T[ACGT]AGG
EcoRI 97 GAATTC G^AATTC
HgaI 15 GACGC GACGC(5/10)
HinfI 1146 GAGTC G^A[ACGT]TC
KspI 860 CCGCGG CCGC^GG
MflI 917 AGATCT [GA]^GATC[CT]
MluNI 60 TGGCCA TGG^CCA
MscI 60 TGGCCA TGG^CCA
MseI 701 TTAA T^TAA
MvnI 861 CGCG CG^CG
PflFI 373 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1192 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1146 GAGTC GAGTC(4/5)
PvuII 37 CAGCTG CAG^CTG
SacII 860 CCGCGG CCGC^GG
SapI 694 GAAGAGC GCTCTTC(1/4)
SbfI 556 CCTGCAGG CCTGCA^GG
SfaNI 112 GATGC GCATC(5/9)
SmaI 947 CCCGGG CCC^GGG
Sse8387I 556 CCTGCAGG CCTGCA^GG
StuI 614 AGGCCT AGG^CCT
TaqI 1246 TCGA T^CGA
Tru9I 701 TTAA T^TAA
Tsp45I 13 GTGAC ^GT[GC]AC
Tth111I 373 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1246 TCGA T^CGA
Van91I 1192 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 917 AGATCT [GA]^GATC[CT]
XmaCI 947 CCCGGG C^CCGGG
XmaI 947 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 195 GTAC GT^AC
456 GTAC
ApaI 754 GGGCCC GGGCC^C
1270 GGGCCC
BanII 754 GGGCCC G[GA]GC[CT]^C
1270 GGGCCC
BsgI 407 GTGCAG GTGCAG(16/14)
548 GTGCAG
BsmAI 379 GTCTC GTCTC(1/5)
495 GTCTC
BsrDI 1152 CATTGC GCAATG(2/0)
56 GCAATG
BsrFI 637 GCCGGC [GA]^CCGG[CT]
1047 GCCGGC
BstAPI 24 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
550 GCAGCTCCTGC
Cfr10I 637 GCCGGC [GA]^CCGG[CT]
1047 GCCGGC
DpnII 918 GATC ^GATC
1013 GATC
DsaI 860 CCGCGG C^C[GA][CT]GG
1253 CCGTGG
MaeIII 13 GTGAC ^GT[ACGT]AC
319 GTTAC
MboI 918 GATC ^GATC
1013 GATC
MspA1I 37 CAGCTG C[AC]G^C[GT]G
860 CCGCGG
NaeI 637 GCCGGC GCC^GGC
1047 GCCGGC
NdeII 918 GATC ^GATC
1013 GATC
NgoMIV 637 GCCGGC G^CCGGC
1047 GCCGGC
NspI 357 ACATGC [GA]CATG^[CT]
458 ACATGC
PpuMI 950 GGGACCT [GA]G^G[AT]CC[CT]
993 GGGACCC
RsaI 195 GTAC GT^AC
456 GTAC
Sau3AI 918 GATC ^GATC
1013 GATC
Tsp509I 98 AATT ^AATT
166 AATT
XcmI 329 CCATGCCAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
758 CCAAGGAAGAGTTGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaI
AvaII BbvI BcnI BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeI
MboII MnlI MspI MvaI MwoI
NciI NlaIII NlaIV PstI Sau96I
ScrFI SfcI StyI TseI TspRI