################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FbaI FseI FspI HaeII HhaI HinP1I HindIII HpaI KasI MaeI MamI MfeI MluI MroI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 991 GACGTC GACGT^C Acc65I 752 GGTACC G^GTACC AccI 15 GTCTAC GT^[AC][GT]AC AflIII 707 ACGTGT A^C[GA][CT]GT AlwI 795 GATCC GGATC(4/5) Asp718I 752 GGTACC G^GTACC AspI 825 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 804 TGGCCA TGG^CCA BbsI 589 GAAGAC GAAGAC(2/6) BcnI 645 CCCGG CC^[GC]GG BglII 665 AGATCT A^GATCT BpmI 208 CTGGAG CTGGAG(16/14) BpuAI 589 GAAGAC GAAGAC(2/6) BsaAI 652 TACGTA [CT]AC^GT[GA] BsgI 241 CTGCAC GTGCAG(16/14) BsmBI 939 CGTCTC CGTCTC(1/5) Bsp1407I 731 TGTACA T^GTACA BspMI 427 ACCTGC ACCTGC(4/8) BsrBI 883 GAGCGG CCGCTC(-3/-3) BsrDI 166 CATTGC GCAATG(2/0) BsrGI 731 TGTACA T^GTACA BstAPI 952 GCATATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 70 TTCGAA TT^CGAA BstXI 737 CCATGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 140 GCCCGC GC[ACGT]^[ACGT]GC DraII 521 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 95 CCGCGG C^C[GA][CT]GG EaeI 804 TGGCCA [CT]^GGCC[GA] EcoO109I 521 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 945 GAATTC G^AATTC EcoRV 277 GATATC GAT^ATC EcoT22I 409 ATGCAT ATGCA^T HincII 994 GTCAAC GT[CT]^[GA]AC HindII 994 GTCAAC GT[CT]^[GA]AC KpnI 752 GGTACC GGTAC^C KspI 95 CCGCGG CCGC^GG MaeIII 332 GTAAC ^GT[ACGT]AC MluNI 804 TGGCCA TGG^CCA MscI 804 TGGCCA TGG^CCA NciI 645 CCCGG CC^[GC]GG NdeI 953 CATATG CA^TATG NsiI 409 ATGCAT ATGCA^T NspI 407 ACATGC [GA]CATG^[CT] NspV 70 TTCGAA TT^CGAA PflFI 825 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 521 GGGACCT [GA]G^G[AT]CC[CT] PshAI 564 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 95 CCGCGG CCGC^GG SfcI 926 CTACAG C^T[GA][CT]AG SfuI 70 TTCGAA TT^CGAA SmlI 526 CTCAAG C^T[CT][GA]AG SnaBI 652 TACGTA TAC^GTA SspBI 731 TGTACA T^GTACA Tth111I 825 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 50 CGCG CG^CG 96 CGCG AcyI 592 GACGCC G[GA]^CG[CT]C 991 GACGTC AfaI 732 GTAC GT^AC 753 GTAC BsaHI 592 GACGCC G[GA]^CG[CT]C 991 GACGTC BsaI 215 GAGACC GGTCTC(1/5) 367 GAGACC BsmFI 404 GGGAC GGGAC(10/14) 521 GGGAC BsmI 411 GCATTC GAATGC(1/-1) 860 GCATTC BsrI 677 CCAGT ACTGG(1/-1) 807 CCAGT BstF5I 852 CATCC GGATG(2/0) 913 CATCC BstUI 50 CGCG CG^CG 96 CGCG BstYI 665 AGATCT [GA]^GATC[CT] 794 AGATCC EcoT14I 836 CCAAGG C^C[AT][AT]GG 916 CCTTGG FokI 852 CATCC GGATG(9/13) 913 CATCC Hin1I 592 GACGCC G[GA]^CG[CT]C 991 GACGTC HphI 364 GGTGA GGTGA(8/7) 723 GGTGA MflI 665 AGATCT [GA]^GATC[CT] 794 AGATCC MseI 548 TTAA T^TAA 1025 TTAA MslI 493 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 738 CATGTTGGTG MvnI 50 CGCG CG^CG 96 CGCG PvuII 282 CAGCTG CAG^CTG 979 CAGCTG RsaI 732 GTAC GT^AC 753 GTAC SfaNI 726 GATGC GCATC(5/9) 237 GCATC StyI 836 CCAAGG C^C[AT][AT]GG 916 CCTTGG TaqI 71 TCGA T^CGA 943 TCGA TfiI 295 GAATC G^A[AT]TC 619 GATTC Tru9I 548 TTAA T^TAA 1025 TTAA TthHB8I 71 TCGA T^CGA 943 TCGA XhoII 665 AGATCT [GA]^GATC[CT] 794 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AcsI AluI ApoI AvaI AvaII BanI BanII BbvI BmyI BsaJI BseRI BsiYI BslI BsmAI BsoBI Bsp1286I BssKI BstNI Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI HaeIII HapII HgaI HinfI HpaII ItaI Ksp632I MaeII MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TseI Tsp509I TspRI