Restriction map for
pF1KE0951
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AgeI Alw44I AlwNI Aor51HI
ApaLI AscI Asp700I Asp718I AvrII
BalI BbeI BbrPI BbvCI BcgI
BclI BglII BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspMI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I StuI
SwaI Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 75 CGCG CG^CG
AflII 976 CTTAAG C^TTAAG
AflIII 668 ACATGT A^C[GA][CT]GT
AhdI 667 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 658 CCTGAGG CC^T[ACGT]AGG
ApaI 1145 GGGCCC GGGCC^C
AseI 837 ATTAAT AT^TAAT
AsnI 837 ATTAAT AT^TAAT
AspEI 667 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 586 GTGCTC G[AT]GC[AT]^C
AspI 823 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 66 CCCGGG C^[CT]CG[GA]G
AviII 622 TGCGCA TGC^GCA
BamHI 849 GGATCC G^GATCC
BanII 1145 GGGCCC G[GA]GC[CT]^C
BciVI 930 GGATAC GTATCC(6/5)
BfrI 976 CTTAAG C^TTAAG
BglI 55 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1070 GAGACC GGTCTC(1/5)
BsgI 1214 GTGCAG GTGCAG(16/14)
BsiHKAI 586 GTGCTC G[AT]GC[AT]^C
BsmAI 1070 GAGAC GTCTC(1/5)
BsoBI 66 CCCGGG C^[CT]CG[GA]G
BspHI 590 TCATGA T^CATGA
BspLU11I 668 ACATGT A^CATGT
BsrBI 935 CCGCTC CCGCTC(-3/-3)
BstEII 48 GGTCACC G^GT[ACGT]ACC
BstPI 48 GGTCACC G^GT[ACGT]ACC
BstUI 75 CGCG CG^CG
BstYI 849 GGATCC [GA]^GATC[CT]
Bsu36I 658 CCTGAGG CC^T[ACGT]AGG
Eam1105I 667 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 658 CCTGAGG CC^T[ACGT]AGG
EcoNI 1305 CCTAGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 48 GGTCACC G^GT[ACGT]ACC
FauI 1010 CCCGC CCCGC(4/6)
FspI 622 TGCGCA TGC^GCA
HindIII 973 AAGCTT A^AGCTT
MflI 849 GGATCC [GA]^GATC[CT]
MslI 466 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 983 CAGCGG C[AC]G^C[GT]G
MvnI 75 CGCG CG^CG
NcoI 1035 CCATGG C^CATGG
NheI 1063 GCTAGC G^CTAGC
NspI 668 ACATGT [GA]CATG^[CT]
PflFI 823 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 199 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 837 ATTAAT AT^TAAT
PstI 422 CTGCAG CTGCA^G
RcaI 590 TCATGA T^CATGA
SapI 41 GCTCTTC GCTCTTC(1/4)
SmaI 66 CCCGGG CCC^GGG
SmlI 976 CTTAAG C^T[CT][GA]AG
SspI 773 AATATT AAT^ATT
TfiI 504 GAATC G^A[AT]TC
Tth111I 823 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 849 GGATCC [GA]^GATC[CT]
XmaCI 66 CCCGGG C^CCGGG
XmaI 66 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 15 GGTGCC G^G[CT][GA]CC
1248 GGTGCC
BbsI 594 GAAGAC GAAGAC(2/6)
1323 GAAGAC
BlpI 123 GCTGAGC GC^T[ACGT]AGC
1174 GCTGAGC
Bpu1102I 123 GCTGAGC GC^T[ACGT]AGC
1174 GCTGAGC
BpuAI 594 GAAGAC GAAGAC(2/6)
1323 GAAGAC
Bsp1407I 179 TGTACA T^GTACA
921 TGTACA
BsrGI 179 TGTACA T^GTACA
921 TGTACA
BsrI 95 ACTGG ACTGG(1/-1)
56 CCAGT
BstXI 818 CCAAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1253 CCAAGGCAGTGG
CelII 123 GCTGAGC GC^T[ACGT]AGC
1174 GCTGAGC
CfoI 121 GCGC GCG^C
623 GCGC
DsaI 892 CCGTGG C^C[GA][CT]GG
1035 CCATGG
EcoRI 758 GAATTC G^AATTC
955 GAATTC
HhaI 121 GCGC GCG^C
623 GCGC
HinP1I 121 GCGC G^CGC
623 GCGC
PleI 560 GACTC GAGTC(4/5)
145 GAGTC
SspBI 179 TGTACA T^GTACA
921 TGTACA
TaqI 101 TCGA T^CGA
137 TCGA
TthHB8I 101 TCGA T^CGA
137 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI Tru9I
TseI Tsp45I Tsp509I TspRI