Restriction map for
pF1KE0960
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AviII AvrII BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaI BseAI BsgI
BsiWI BsmBI BspDI BspEI BspHI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I ClaI CpoI DraI Eam1105I
Eco47III EcoNI EcoO65I EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 17 GGTACC G^GTACC
AccI 976 GTAGAC GT^[AC][GT]AC
AcsI 897 GAATTC [GA]^AATT[CT]
AcyI 131 GGCGTC G[GA]^CG[CT]C
AflIII 713 ACATGT A^C[GA][CT]GT
AocI 503 CCTTAGG CC^T[ACGT]AGG
ApoI 897 GAATTC [GA]^AATT[CT]
Asp718I 17 GGTACC G^GTACC
AspI 128 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 563 CTCGAG C^[CT]CG[GA]G
BalI 708 TGGCCA TGG^CCA
BanI 17 GGTACC G^G[CT][GA]CC
BbvCI 439 GCTGAGG CCTCAGC(-5/-2)
BpmI 1476 CTCCAG CTGGAG(16/14)
BsaHI 131 GGCGTC G[GA]^CG[CT]C
BsmAI 1024 GTCTC GTCTC(1/5)
BsmI 288 GCATTC GAATGC(1/-1)
BsoBI 563 CTCGAG C^[CT]CG[GA]G
Bsp1407I 531 TGTACA T^GTACA
BspLU11I 713 ACATGT A^CATGT
BspMI 776 ACCTGC ACCTGC(4/8)
BsrBI 387 CCGCTC CCGCTC(-3/-3)
BsrFI 209 ACCGGC [GA]^CCGG[CT]
BsrGI 531 TGTACA T^GTACA
BstYI 1184 GGATCT [GA]^GATC[CT]
Bsu36I 503 CCTTAGG CC^T[ACGT]AGG
Cfr10I 209 ACCGGC [GA]^CCGG[CT]
DraIII 793 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 86 GACGAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 705 CCATGG C^C[GA][CT]GG
EagI 522 CGGCCG C^GGCCG
EclXI 522 CGGCCG C^GGCCG
Eco52I 522 CGGCCG C^GGCCG
Eco81I 503 CCTTAGG CC^T[ACGT]AGG
EcoRI 897 GAATTC G^AATTC
EcoRV 1486 GATATC GAT^ATC
HaeII 1283 GGCGCT [GA]GCGC^[CT]
Hin1I 131 GGCGTC G[GA]^CG[CT]C
HincII 541 GTTGAC GT[CT]^[GA]AC
HindII 541 GTTGAC GT[CT]^[GA]AC
KpnI 17 GGTACC GGTAC^C
MflI 1184 GGATCT [GA]^GATC[CT]
MluNI 708 TGGCCA TGG^CCA
MscI 708 TGGCCA TGG^CCA
MseI 584 TTAA T^TAA
NcoI 705 CCATGG C^CATGG
PaeR7I 563 CTCGAG C^TCGAG
PflFI 128 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1474 GACTC GAGTC(4/5)
PshAI 1474 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 910 GAGCTC GAGCT^C
SfcI 228 CTACAG C^T[GA][CT]AG
SphI 1373 GCATGC GCATG^C
SspBI 531 TGTACA T^GTACA
TfiI 1130 GATTC G^A[AT]TC
Tru9I 584 TTAA T^TAA
Tsp509I 898 AATT ^AATT
Tth111I 128 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 546 CCATCGCAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 563 CTCGAG C^TCGAG
XhoII 1184 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Asp700I 880 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1414 GAAGCCCTTC
AvaII 515 GGACC G^G[AT]CC
1192 GGTCC
BanII 560 GGGCTC G[GA]GC[CT]^C
910 GAGCTC
BlpI 1393 GCTAAGC GC^T[ACGT]AGC
1450 GCTGAGC
Bpu1102I 1393 GCTAAGC GC^T[ACGT]AGC
1450 GCTGAGC
BsaWI 119 ACCGGA [AT]^CCGG[AT]
194 ACCGGA
BseRI 24 CTCCTC GAGGAG(10/8)
363 GAGGAG
BsiEI 192 CGACCG CG[GA][CT]^CG
522 CGGCCG
CelII 1393 GCTAAGC GC^T[ACGT]AGC
1450 GCTGAGC
DraII 514 GGGACCT [GA]G^G[ACGT]CC[CT]
1191 GGGTCCT
EaeI 522 CGGCCG [CT]^GGCC[GA]
708 TGGCCA
EcoO109I 514 GGGACCT [GA]G^G[ACGT]CC[CT]
1191 GGGTCCT
EcoT14I 705 CCATGG C^C[AT][AT]GG
1406 CCTTGG
HinfI 1130 GATTC G^A[ACGT]TC
1474 GACTC
MaeII 461 ACGT A^CGT
629 ACGT
MslI 289 CATTCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
960 CACAGCTGTG
NspI 713 ACATGT [GA]CATG^[CT]
1373 GCATGC
PpuMI 514 GGGACCT [GA]G^G[AT]CC[CT]
1191 GGGTCCT
SapI 954 GAAGAGC GCTCTTC(1/4)
1353 GCTCTTC
SspI 883 AATATT AAT^ATT
1235 AATATT
StyI 705 CCATGG C^C[AT][AT]GG
1406 CCTTGG
Tsp45I 116 GTGAC ^GT[GC]AC
1074 GTCAC
TspRI 948 CAGTG CA[GC]TG[ACGT][ACGT]^
987 CAGTG
XmnI 880 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1414 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BbvI BcnI BfaI BmyI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI TseI
TthHB8I