Restriction map for
pF1KE0967
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccII AclI AcyI AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspHI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaHI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I BspDI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII HpaI KasI Ksp632I
KspI MaeII MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1081 GGTACC G^GTACC
AccI 703 GTCTAC GT^[AC][GT]AC
AccIII 351 TCCGGA T^CCGGA
AcsI 1036 AAATTT [GA]^AATT[CT]
AflII 835 CTTAAG C^TTAAG
AflIII 1232 ACATGT A^C[GA][CT]GT
AhdI 673 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1036 AAATTT [GA]^AATT[CT]
Asp718I 1081 GGTACC G^GTACC
AspEI 673 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 1276 GAAGAC GAAGAC(2/6)
BclI 794 TGATCA T^GATCA
BcnI 1085 CCCGG CC^[GC]GG
BfrI 835 CTTAAG C^TTAAG
BpmI 1100 CTCCAG CTGGAG(16/14)
BpuAI 1276 GAAGAC GAAGAC(2/6)
BsaBI 778 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 849 GGTCTC GGTCTC(1/5)
BsaWI 351 TCCGGA [AT]^CCGG[AT]
BseAI 351 TCCGGA T^CCGGA
BseRI 1246 GAGGAG GAGGAG(10/8)
BsgI 752 GTGCAG GTGCAG(16/14)
BsmFI 962 GGGAC GGGAC(10/14)
Bsp1407I 479 TGTACA T^GTACA
BspEI 351 TCCGGA T^CCGGA
BspHI 807 TCATGA T^CATGA
BspLU11I 1232 ACATGT A^CATGT
BspMI 945 ACCTGC ACCTGC(4/8)
BsrGI 479 TGTACA T^GTACA
BstYI 783 GGATCT [GA]^GATC[CT]
Eam1105I 673 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 244 GATATC GAT^ATC
EcoT22I 1139 ATGCAT ATGCA^T
FauI 451 GCGGG CCCGC(4/6)
FbaI 794 TGATCA T^GATCA
HgaI 1393 GACGC GACGC(5/10)
HincII 1510 GTTGAC GT[CT]^[GA]AC
HindII 1510 GTTGAC GT[CT]^[GA]AC
HinfI 1528 GAATC G^A[ACGT]TC
KpnI 1081 GGTACC GGTAC^C
MamI 778 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 783 GGATCT [GA]^GATC[CT]
MroI 351 TCCGGA T^CCGGA
MslI 1494 CACAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1412 CAGCTG C[AC]G^C[GT]G
NciI 1085 CCCGG CC^[GC]GG
NsiI 1139 ATGCAT ATGCA^T
PvuII 1412 CAGCTG CAG^CTG
RcaI 807 TCATGA T^CATGA
SspBI 479 TGTACA T^GTACA
StuI 1312 AGGCCT AGG^CCT
TfiI 1528 GAATC G^A[AT]TC
XhoII 783 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 171 GCGG CCGC(-3/-1)
451 GCGG
AlwI 783 GGATC GGATC(4/5)
1386 GGATC
AvaII 535 GGACC G^G[AT]CC
1216 GGACC
BalI 936 TGGCCA TGG^CCA
1257 TGGCCA
BsmAI 1449 GAGAC GTCTC(1/5)
850 GTCTC
BsrDI 1011 CATTGC GCAATG(2/0)
1354 GCAATG
Cfr13I 535 GGACC G^G[ACGT]CC
1216 GGACC
DdeI 80 CTCAG C^T[ACGT]AG
395 CTAAG
EaeI 936 TGGCCA [CT]^GGCC[GA]
1257 TGGCCA
HapII 352 CCGG C^CGG
1086 CCGG
HpaII 352 CCGG C^CGG
1086 CCGG
MluNI 936 TGGCCA TGG^CCA
1257 TGGCCA
MscI 936 TGGCCA TGG^CCA
1257 TGGCCA
MspI 352 CCGG C^CGG
1086 CCGG
Sau96I 535 GGACC G^G[ACGT]CC
1216 GGACC
SmlI 524 CTCAAG C^T[CT][GA]AG
835 CTTAAG
XcmI 770 CCAGTGTTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1068 CCATGGACAGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI BanI BbvI BfaI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI Cac8I DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PstI RsaI Sau3AI ScrFI SfaNI
SfcI SspI StyI Tru9I TseI
Tsp45I Tsp509I TspRI