################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BbvI BciVI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FauI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PstI PvuI PvuII RcaI RsrII SacI SacII SapI SbfI ScaI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AclI 304 AACGTT AA^CGTT AfaI 229 GTAC GT^AC AluI 455 AGCT AG^CT ApaI 84 GGGCCC GGGCC^C AseI 429 ATTAAT AT^TAAT AsnI 429 ATTAAT AT^TAAT BanII 84 GGGCCC G[GA]GC[CT]^C BbsI 3 GAAGAC GAAGAC(2/6) BcgI 68 GCACAGTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 239 TGATCA T^GATCA BcnI 165 CCGGG CC^[GC]GG BmyI 84 GGGCCC G[AGT]GC[ACT]^C BpuAI 3 GAAGAC GAAGAC(2/6) Bsp1286I 84 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 228 TGTACA T^GTACA BsrGI 228 TGTACA T^GTACA BstXI 315 CCAGGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 240 GATC ^GATC EcoRV 511 GATATC GAT^ATC FbaI 239 TGATCA T^GATCA Fnu4HI 472 GCGGC GC^[ACGT]GC HaeIII 85 GGCC GG^CC HapII 165 CCGG C^CGG HgaI 65 GACGC GACGC(5/10) HinfI 480 GATTC G^A[ACGT]TC HpaII 165 CCGG C^CGG HphI 355 GGTGA GGTGA(8/7) ItaI 472 GCGGC GC^[ACGT]GC MaeII 305 ACGT A^CGT MboI 240 GATC ^GATC MboII 3 GAAGA GAAGA(8/7) MslI 118 CACATCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 470 CAGCGG C[AC]G^C[GT]G MspI 165 CCGG C^CGG NciI 165 CCGGG CC^[GC]GG NdeII 240 GATC ^GATC PshBI 429 ATTAAT AT^TAAT Psp1406I 304 AACGTT AA^CGTT RsaI 229 GTAC GT^AC Sau3AI 240 GATC ^GATC SexAI 387 ACCAGGT A^CC[AT]GGT SmlI 410 CTCAAG C^T[CT][GA]AG SspBI 228 TGTACA T^GTACA TfiI 480 GATTC G^A[AT]TC TspRI 254 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 28 GTCGAC GT^[AC][GT]AC 46 GTCGAC BsaI 384 GAGACC GGTCTC(1/5) 326 GGTCTC BsiYI 98 CCCTGCGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 99 CCTGCGGAGGG BslI 98 CCCTGCGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 99 CCTGCGGAGGG BsmFI 63 GGGAC GGGAC(10/14) 107 GGGAC BsrI 198 ACTGG ACTGG(1/-1) 255 ACTGG BstNI 315 CCAGG CC^[AT]GG 388 CCAGG Cac8I 486 GCAAGC GC[ACGT]^[ACGT]GC 564 GCAGGC Cfr13I 84 GGGCC G^G[ACGT]CC 85 GGCCC DdeI 13 CTCAG C^T[ACGT]AG 133 CTCAG DraII 83 AGGGCCC [GA]G^G[ACGT]CC[CT] 84 GGGCCCC EcoO109I 83 AGGGCCC [GA]G^G[ACGT]CC[CT] 84 GGGCCCC EcoRII 315 CCAGG ^CC[AT]GG 388 CCAGG MseI 430 TTAA T^TAA 500 TTAA MvaI 315 CCAGG CC^[AT]GG 388 CCAGG NlaIII 226 CATG CATG^ 237 CATG SalI 28 GTCGAC G^TCGAC 46 GTCGAC Sau96I 84 GGGCC G^G[ACGT]CC 85 GGCCC TaqI 29 TCGA T^CGA 47 TCGA Tru9I 430 TTAA T^TAA 500 TTAA TthHB8I 29 TCGA T^CGA 47 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AciI AcsI ApoI BsmAI BssKI BstF5I FokI HincII HindII MaeIII MnlI NlaIV ScrFI Tsp509I