Restriction map for
pF1KB3443
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BciVI BclI BcnI BfaI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrI BssHII
BssKI Bst1107I BstEII BstNI BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HapII HgaI Hin1I HincII
HindII HpaI HpaII HphI KasI
KspI MaeI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvaI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI ScrFI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tsp45I TspRI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 428 GGTACC G^GTACC
AflII 52 CTTAAG C^TTAAG
Asp718I 428 GGTACC G^GTACC
AvaII 711 GGACC G^G[AT]CC
BanI 428 GGTACC G^G[CT][GA]CC
BanII 303 GAGCCC G[GA]GC[CT]^C
BbsI 639 GTCTTC GAAGAC(2/6)
BbvCI 396 GCTGAGG CCTCAGC(-5/-2)
BbvI 327 GCAGC GCAGC(8/12)
BfrI 52 CTTAAG C^TTAAG
BglII 578 AGATCT A^GATCT
BmyI 303 GAGCCC G[AGT]GC[ACT]^C
BpuAI 639 GTCTTC GAAGAC(2/6)
BsiYI 646 CCACGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 646 CCACGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 303 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 191 TGTACA T^GTACA
BspHI 573 TCATGA T^CATGA
BspMI 249 ACCTGC ACCTGC(4/8)
BsrGI 191 TGTACA T^GTACA
BssSI 647 CACGAG CACGAG(-5/-1)
BstAPI 548 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 218 GGATG GGATG(2/0)
BstYI 578 AGATCT [GA]^GATC[CT]
CfoI 93 GCGC GCG^C
DraIII 647 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 13 GAAGAG CTCTTC(1/4)
Fnu4HI 327 GCAGC GC^[ACGT]GC
FokI 218 GGATG GGATG(9/13)
HaeII 92 AGCGCC [GA]GCGC^[CT]
HaeIII 626 GGCC GG^CC
HhaI 93 GCGC GCG^C
HinP1I 93 GCGC G^CGC
HindIII 142 AAGCTT A^AGCTT
ItaI 327 GCAGC GC^[ACGT]GC
KpnI 428 GGTACC GGTAC^C
Ksp632I 13 GAAGAG CTCTTC(1/4)
MflI 578 AGATCT [GA]^GATC[CT]
MslI 552 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 392 ACATGC [GA]CATG^[CT]
PflMI 646 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 251 CTGCAG CTGCA^G
RcaI 573 TCATGA T^CATGA
SfcI 251 CTGCAG C^T[GA][CT]AG
SmlI 52 CTTAAG C^T[CT][GA]AG
SspBI 191 TGTACA T^GTACA
TseI 327 GCAGC G^C[AT]GC
Van91I 646 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 412 CCATATATTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 578 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
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AcsI 69 GAATTT [GA]^AATT[CT]
203 AAATTC
ApoI 69 GAATTT [GA]^AATT[CT]
203 AAATTC
BcgI 386 CGATTAACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
327 GCAGCTTCTTCG
Cac8I 232 GCTGGC GC[ACGT]^[ACGT]GC
697 GCTGGC
Cfr13I 626 GGCCC G^G[ACGT]CC
711 GGACC
DdeI 97 CTGAG C^T[ACGT]AG
397 CTGAG
HinfI 281 GAATC G^A[ACGT]TC
570 GATTC
MseI 53 TTAA T^TAA
389 TTAA
Sau96I 626 GGCCC G^G[ACGT]CC
711 GGACC
TfiI 281 GAATC G^A[AT]TC
570 GATTC
Tru9I 53 TTAA T^TAA
389 TTAA
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AfaI AluI BstBI DpnII MboI
MboII MnlI MwoI NdeII NlaIII
NlaIV NspV RsaI Sau3AI SfaNI
SfuI TaqI Tsp509I TthHB8I