Restriction map for
pF1KB3432
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# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MflI
MluI MroI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tsp509I
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AlwNI 209 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 16 GAGCTC G[AT]GC[AT]^C
BalI 152 TGGCCA TGG^CCA
BanII 16 GAGCTC G[GA]GC[CT]^C
BbvCI 265 GCTGAGG CCTCAGC(-5/-2)
BglI 120 GCCTTTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 16 GAGCTC G[AGT]GC[ACT]^C
BsiHKAI 16 GAGCTC G[AT]GC[AT]^C
BsmAI 10 GTCTC GTCTC(1/5)
Bsp1286I 16 GAGCTC G[AGT]GC[ACT]^C
BspMI 201 ACCTGC ACCTGC(4/8)
BsrDI 182 GCAATG GCAATG(2/0)
BstAPI 211 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI 266 CTGAG C^T[ACGT]AG
DpnII 81 GATC ^GATC
EaeI 152 TGGCCA [CT]^GGCC[GA]
HincII 160 GTCAAC GT[CT]^[GA]AC
HindII 160 GTCAAC GT[CT]^[GA]AC
HphI 60 GGTGA GGTGA(8/7)
MaeII 158 ACGT A^CGT
MboI 81 GATC ^GATC
MluNI 152 TGGCCA TGG^CCA
MscI 152 TGGCCA TGG^CCA
MseI 95 TTAA T^TAA
MslI 49 CACGACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 81 GATC ^GATC
NlaIII 321 CATG CATG^
PflMI 208 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 307 GAGTC GAGTC(4/5)
PpuMI 234 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 16 GAGCTC GAGCT^C
Sau3AI 81 GATC ^GATC
SfaNI 26 GCATC GCATC(5/9)
TfiI 298 GAATC G^A[AT]TC
Tru9I 95 TTAA T^TAA
TspRI 249 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 208 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaII 199 GGACC G^G[AT]CC
235 GGTCC
BpmI 206 CTCCAG CTGGAG(16/14)
260 CTCCAG
BsaJI 149 CCCTGG C^C[ACGT][ACGT]GG
302 CCGAGG
BseRI 268 GAGGAG GAGGAG(10/8)
304 GAGGAG
BsgI 46 CTGCAC GTGCAG(16/14)
221 CTGCAC
BsiYI 208 CCAGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
226 CCAGCAGGAGG
BslI 208 CCAGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
226 CCAGCAGGAGG
BsrFI 103 GCCGGT [GA]^CCGG[CT]
193 GCCGGT
BssKI 130 CCTGG ^CC[ACGT]GG
150 CCTGG
BstNI 130 CCTGG CC^[AT]GG
150 CCTGG
Cac8I 18 GCTCGC GC[ACGT]^[ACGT]GC
22 GCCTGC
Cfr10I 103 GCCGGT [GA]^CCGG[CT]
193 GCCGGT
DraII 146 AGGCCCT [GA]G^G[ACGT]CC[CT]
234 AGGTCCT
EcoO109I 146 AGGCCCT [GA]G^G[ACGT]CC[CT]
234 AGGTCCT
EcoRII 130 CCTGG ^CC[AT]GG
150 CCTGG
HapII 104 CCGG C^CGG
194 CCGG
HinfI 298 GAATC G^A[ACGT]TC
307 GAGTC
HpaII 104 CCGG C^CGG
194 CCGG
MaeIII 61 GTGAC ^GT[ACGT]AC
67 GTCAC
MboII 273 GAAGA GAAGA(8/7)
295 GAAGA
MspA1I 212 CAGCTG C[AC]G^C[GT]G
263 CAGCTG
MspI 104 CCGG C^CGG
194 CCGG
MvaI 130 CCTGG CC^[AT]GG
150 CCTGG
NlaIV 170 GGAGCC GG[ACGT]^[ACGT]CC
190 GGGGCC
PvuII 212 CAGCTG CAG^CTG
263 CAGCTG
ScrFI 130 CCTGG CC^[ACGT]GG
150 CCTGG
Tsp45I 61 GTGAC ^GT[GC]AC
67 GTCAC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AluI BbvI Cfr13I Fnu4HI HaeIII
ItaI MnlI MwoI Sau96I TseI