Restriction map for pF1KB3479
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccIII      AciI        AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglII       BlnI        
BsaBI       BsaHI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BsmFI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      BstXI       BstYI       
Bsu36I      Cfr10I      ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRV       FauI        FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MroI        
MunI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccII        1120  CGCG                 CG^CG
AcsI          248  GAATTC               [GA]^AATT[CT]
AlwI          177  GGATC                GGATC(4/5)
ApoI          248  GAATTC               [GA]^AATT[CT]
Asp700I       969  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI          202  GAAGAC               GAAGAC(2/6)
BfaI         1003  CTAG                 C^TAG
BglI          754  GCCGAAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          679  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      679  GCTGAGC              GC^T[ACGT]AGC
BpuAI         202  GAAGAC               GAAGAC(2/6)
BsaAI        1018  TACGTG               [CT]AC^GT[GA]
BsaWI         434  ACCGGA               [AT]^CCGG[AT]
BseRI         341  CTCCTC               GAGGAG(10/8)
BsmI          998  GCATTC               GAATGC(1/-1)
BspMI         808  ACCTGC               ACCTGC(4/8)
BssSI          40  CACGAG               CACGAG(-5/-1)
BstBI          94  TTCGAA               TT^CGAA
BstEII        736  GGTGACC              G^GT[ACGT]ACC
BstPI         736  GGTGACC              G^GT[ACGT]ACC
BstUI        1120  CGCG                 CG^CG
CelII         679  GCTGAGC              GC^T[ACGT]AGC
CfoI         1119  GCGC                 GCG^C
DpnII         178  GATC                 ^GATC
DraI           66  TTTAAA               TTT^AAA
DsaI          662  CCATGG               C^C[GA][CT]GG
EcoO65I       736  GGTGACC              G^GT[ACGT]ACC
EcoRI         248  GAATTC               G^AATTC
EcoT22I      1148  ATGCAT               ATGCA^T
HapII         435  CCGG                 C^CGG
HhaI         1119  GCGC                 GCG^C
HinP1I       1119  GCGC                 G^CGC
HincII        826  GTTGAC               GT[CT]^[GA]AC
HindII        826  GTTGAC               GT[CT]^[GA]AC
HpaII         435  CCGG                 C^CGG
MaeI         1003  CTAG                 C^TAG
MaeII        1019  ACGT                 A^CGT
MboI          178  GATC                 ^GATC
MseI           67  TTAA                 T^TAA
MspI          435  CCGG                 C^CGG
MvnI         1120  CGCG                 CG^CG
NcoI          662  CCATGG               C^CATGG
NdeII         178  GATC                 ^GATC
NsiI         1148  ATGCAT               ATGCA^T
NspV           94  TTCGAA               TT^CGAA
PpuMI         476  AGGACCC              [GA]G^G[AT]CC[CT]
SapI          145  GAAGAGC              GCTCTTC(1/4)
Sau3AI        178  GATC                 ^GATC
SfuI           94  TTCGAA               TT^CGAA
SmlI          162  CTTGAG               C^T[CT][GA]AG
SphI         1146  GCATGC               GCATG^C
SspI          698  AATATT               AAT^ATT
Tru9I          67  TTAA                 T^TAA
XmnI          969  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          213  GTATAC               GT^[AC][GT]AC
              936  GTATAC              
AfaI          375  GTAC                 GT^AC
              432  GTAC                
AlwNI         290  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              804  CAGCACCTG           
AspHI         682  GAGCAC               G[AT]GC[AT]^C
             1170  GTGCTC              
AvaII           3  GGACC                G^G[AT]CC
              477  GGACC               
BalI          138  TGGCCA               TGG^CCA
              365  TGGCCA              
BanI          782  GGCACC               G^G[CT][GA]CC
              962  GGCACC              
BanII          56  GAGCCC               G[GA]GC[CT]^C
              338  GGGCTC              
BpmI          108  CTGGAG               CTGGAG(16/14)
              626  CTGGAG              
BsiHKAI       682  GAGCAC               G[AT]GC[AT]^C
             1170  GTGCTC              
BsmAI         310  GTCTC                GTCTC(1/5)
              501  GTCTC               
Bst1107I      213  GTATAC               GTA^TAC
              936  GTATAC              
BstZ17I       213  GTATAC               GTA^TAC
              936  GTATAC              
DraII         476  AGGACCC              [GA]G^G[ACGT]CC[CT]
              603  AGGCCCC             
DraIII       1069  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1155  CACAGAGTG           
EaeI          138  TGGCCA               [CT]^GGCC[GA]
              365  TGGCCA              
EcoO109I      476  AGGACCC              [GA]G^G[ACGT]CC[CT]
              603  AGGCCCC             
HphI          513  GGTGA                GGTGA(8/7)
              736  GGTGA               
MluNI         138  TGGCCA               TGG^CCA
              365  TGGCCA              
MscI          138  TGGCCA               TGG^CCA
              365  TGGCCA              
MslI          378  CATGCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              874  CATTACTGTG          
MspA1I        445  CAGCTG               C[AC]G^C[GT]G
             1140  CAGCTG              
NspI          377  ACATGC               [GA]CATG^[CT]
             1146  GCATGC              
PstI          801  CTGCAG               CTGCA^G
             1179  CTGCAG              
PvuII         445  CAGCTG               CAG^CTG
             1140  CAGCTG              
RsaI          375  GTAC                 GT^AC
              432  GTAC                
SfcI          801  CTGCAG               C^T[GA][CT]AG
             1179  CTGCAG              
TspRI         653  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1238  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AluI        BbvI        BmyI        BsaJI       BsgI        
BsiYI       BslI        Bsp1286I    BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
Ksp632I     MaeIII      MboII       MnlI        MvaI        
MwoI        NlaIII      NlaIV       Sau96I      ScrFI       
SfaNI       StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TthHB8I     
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