################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BstXI BstYI Bsu36I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV FauI FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccII 1120 CGCG CG^CG AcsI 248 GAATTC [GA]^AATT[CT] AlwI 177 GGATC GGATC(4/5) ApoI 248 GAATTC [GA]^AATT[CT] Asp700I 969 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 202 GAAGAC GAAGAC(2/6) BfaI 1003 CTAG C^TAG BglI 754 GCCGAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 679 GCTGAGC GC^T[ACGT]AGC Bpu1102I 679 GCTGAGC GC^T[ACGT]AGC BpuAI 202 GAAGAC GAAGAC(2/6) BsaAI 1018 TACGTG [CT]AC^GT[GA] BsaWI 434 ACCGGA [AT]^CCGG[AT] BseRI 341 CTCCTC GAGGAG(10/8) BsmI 998 GCATTC GAATGC(1/-1) BspMI 808 ACCTGC ACCTGC(4/8) BssSI 40 CACGAG CACGAG(-5/-1) BstBI 94 TTCGAA TT^CGAA BstEII 736 GGTGACC G^GT[ACGT]ACC BstPI 736 GGTGACC G^GT[ACGT]ACC BstUI 1120 CGCG CG^CG CelII 679 GCTGAGC GC^T[ACGT]AGC CfoI 1119 GCGC GCG^C DpnII 178 GATC ^GATC DraI 66 TTTAAA TTT^AAA DsaI 662 CCATGG C^C[GA][CT]GG EcoO65I 736 GGTGACC G^GT[ACGT]ACC EcoRI 248 GAATTC G^AATTC EcoT22I 1148 ATGCAT ATGCA^T HapII 435 CCGG C^CGG HhaI 1119 GCGC GCG^C HinP1I 1119 GCGC G^CGC HincII 826 GTTGAC GT[CT]^[GA]AC HindII 826 GTTGAC GT[CT]^[GA]AC HpaII 435 CCGG C^CGG MaeI 1003 CTAG C^TAG MaeII 1019 ACGT A^CGT MboI 178 GATC ^GATC MseI 67 TTAA T^TAA MspI 435 CCGG C^CGG MvnI 1120 CGCG CG^CG NcoI 662 CCATGG C^CATGG NdeII 178 GATC ^GATC NsiI 1148 ATGCAT ATGCA^T NspV 94 TTCGAA TT^CGAA PpuMI 476 AGGACCC [GA]G^G[AT]CC[CT] SapI 145 GAAGAGC GCTCTTC(1/4) Sau3AI 178 GATC ^GATC SfuI 94 TTCGAA TT^CGAA SmlI 162 CTTGAG C^T[CT][GA]AG SphI 1146 GCATGC GCATG^C SspI 698 AATATT AAT^ATT Tru9I 67 TTAA T^TAA XmnI 969 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 213 GTATAC GT^[AC][GT]AC 936 GTATAC AfaI 375 GTAC GT^AC 432 GTAC AlwNI 290 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG 804 CAGCACCTG AspHI 682 GAGCAC G[AT]GC[AT]^C 1170 GTGCTC AvaII 3 GGACC G^G[AT]CC 477 GGACC BalI 138 TGGCCA TGG^CCA 365 TGGCCA BanI 782 GGCACC G^G[CT][GA]CC 962 GGCACC BanII 56 GAGCCC G[GA]GC[CT]^C 338 GGGCTC BpmI 108 CTGGAG CTGGAG(16/14) 626 CTGGAG BsiHKAI 682 GAGCAC G[AT]GC[AT]^C 1170 GTGCTC BsmAI 310 GTCTC GTCTC(1/5) 501 GTCTC Bst1107I 213 GTATAC GTA^TAC 936 GTATAC BstZ17I 213 GTATAC GTA^TAC 936 GTATAC DraII 476 AGGACCC [GA]G^G[ACGT]CC[CT] 603 AGGCCCC DraIII 1069 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG 1155 CACAGAGTG EaeI 138 TGGCCA [CT]^GGCC[GA] 365 TGGCCA EcoO109I 476 AGGACCC [GA]G^G[ACGT]CC[CT] 603 AGGCCCC HphI 513 GGTGA GGTGA(8/7) 736 GGTGA MluNI 138 TGGCCA TGG^CCA 365 TGGCCA MscI 138 TGGCCA TGG^CCA 365 TGGCCA MslI 378 CATGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 874 CATTACTGTG MspA1I 445 CAGCTG C[AC]G^C[GT]G 1140 CAGCTG NspI 377 ACATGC [GA]CATG^[CT] 1146 GCATGC PstI 801 CTGCAG CTGCA^G 1179 CTGCAG PvuII 445 CAGCTG CAG^CTG 1140 CAGCTG RsaI 375 GTAC GT^AC 432 GTAC SfcI 801 CTGCAG C^T[GA][CT]AG 1179 CTGCAG TspRI 653 CAGTG CA[GC]TG[ACGT][ACGT]^ 1238 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AluI BbvI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI Cac8I Cfr13I DdeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeIII MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I Tsp509I TthHB8I