Restriction map for
pF1KB3480
##################################
# NO CUT ENZYMES ( 160 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AvaII AviII AvrII BbeI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HapII HgaI HhaI HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MaeI MfeI MluI MroI
MseI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tru9I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 785 GACGTC GACGT^C
AcsI 487 AAATTC [GA]^AATT[CT]
AcyI 785 GACGTC G[GA]^CG[CT]C
AfaI 300 GTAC GT^AC
AflIII 302 ACACGT A^C[GA][CT]GT
ApoI 487 AAATTC [GA]^AATT[CT]
AspI 928 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 315 TGGCCA TGG^CCA
BamHI 501 GGATCC G^GATCC
BbrPI 303 CACGTG CAC^GTG
BglI 308 GCCAGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 303 CACGTG [CT]AC^GT[GA]
BsaBI 795 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 785 GACGTC G[GA]^CG[CT]C
BsaI 10 GAGACC GGTCTC(1/5)
BsgI 555 CTGCAC GTGCAG(16/14)
BsmFI 35 GTCCC GGGAC(10/14)
BsrDI 666 CATTGC GCAATG(2/0)
BsrI 313 ACTGG ACTGG(1/-1)
BstYI 501 GGATCC [GA]^GATC[CT]
DrdI 12 GACCCAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 139 CCGTGG C^C[GA][CT]GG
EaeI 315 TGGCCA [CT]^GGCC[GA]
FauI 293 GCGGG CCCGC(4/6)
Hin1I 785 GACGTC G[GA]^CG[CT]C
HincII 934 GTCAAC GT[CT]^[GA]AC
HindII 934 GTCAAC GT[CT]^[GA]AC
MamI 795 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 501 GGATCC [GA]^GATC[CT]
MluNI 315 TGGCCA TGG^CCA
MscI 315 TGGCCA TGG^CCA
MslI 538 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 178 ACATGC [GA]CATG^[CT]
PflFI 928 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 212 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 303 CACGTG CAC^GTG
PmlI 303 CACGTG CAC^GTG
RsaI 300 GTAC GT^AC
SapI 632 GCTCTTC GCTCTTC(1/4)
SfaNI 342 GCATC GCATC(5/9)
SmlI 130 CTTGAG C^T[CT][GA]AG
TfiI 234 GAATC G^A[AT]TC
Tth111I 928 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 212 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 531 CCACAGTCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 501 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AspHI 151 GTGCTC G[AT]GC[AT]^C
370 GTGCTC
BanI 422 GGTGCC G^G[CT][GA]CC
879 GGCACC
BanII 133 GAGCCC G[GA]GC[CT]^C
188 GGGCTC
BbvCI 327 CCTCAGC CCTCAGC(-5/-2)
417 GCTGAGG
BsiHKAI 151 GTGCTC G[AT]GC[AT]^C
370 GTGCTC
BstF5I 516 CATCC GGATG(2/0)
587 CATCC
Cac8I 81 GCTGGC GC[ACGT]^[ACGT]GC
190 GCTCGC
EarI 633 CTCTTC CTCTTC(1/4)
914 CTCTTC
FokI 516 CATCC GGATG(9/13)
587 CATCC
Ksp632I 633 CTCTTC CTCTTC(1/4)
914 CTCTTC
PstI 89 CTGCAG CTGCA^G
717 CTGCAG
PvuII 429 CAGCTG CAG^CTG
845 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AlwI BbvI BmyI
BpmI BsaJI BsiYI BslI BsmAI
Bsp1286I BssKI BstNI DdeI DpnII
EcoRII Fnu4HI HaeIII HinfI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI ScrFI
SfcI TseI Tsp45I Tsp509I TspRI