Restriction map for
pF1KB3557
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbvCI BcgI BciVI
BcnI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I CpoI DraII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HpaI HpaII KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcsI 29 GAATTC [GA]^AATT[CT]
AfaI 742 GTAC GT^AC
AflII 870 CTTAAG C^TTAAG
AflIII 281 ACGTGT A^C[GA][CT]GT
ApoI 29 GAATTC [GA]^AATT[CT]
BanII 541 GGGCTC G[GA]GC[CT]^C
BbrPI 830 CACGTG CAC^GTG
BbvI 444 GCTGC GCAGC(8/12)
BclI 318 TGATCA T^GATCA
BfrI 870 CTTAAG C^TTAAG
BglII 618 AGATCT A^GATCT
BmyI 541 GGGCTC G[AGT]GC[ACT]^C
BpmI 393 CTGGAG CTGGAG(16/14)
BsaI 481 GGTCTC GGTCTC(1/5)
BsgI 688 GTGCAG GTGCAG(16/14)
BsmAI 482 GTCTC GTCTC(1/5)
Bsp1286I 541 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 741 TGTACA T^GTACA
BspDI 187 ATCGAT AT^CGAT
BsrGI 741 TGTACA T^GTACA
BstYI 618 AGATCT [GA]^GATC[CT]
Cac8I 350 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 702 GCGC GCG^C
Cfr13I 234 GGCCC G^G[ACGT]CC
ClaI 187 ATCGAT AT^CGAT
DraI 516 TTTAAA TTT^AAA
DraIII 830 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 29 GAATTC G^AATTC
EcoT14I 291 CCTTGG C^C[AT][AT]GG
FauI 347 CCCGC CCCGC(4/6)
FbaI 318 TGATCA T^GATCA
Fnu4HI 444 GCTGC GC^[ACGT]GC
HhaI 702 GCGC GCG^C
HinP1I 702 GCGC G^CGC
HindIII 337 AAGCTT A^AGCTT
HphI 321 TCACC GGTGA(8/7)
ItaI 444 GCTGC GC^[ACGT]GC
MflI 618 AGATCT [GA]^GATC[CT]
NlaIV 839 GGAACC GG[ACGT]^[ACGT]CC
PflMI 351 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 9 GACTC GAGTC(4/5)
PmaCI 830 CACGTG CAC^GTG
PmlI 830 CACGTG CAC^GTG
PstI 445 CTGCAG CTGCA^G
RsaI 742 GTAC GT^AC
Sau96I 234 GGCCC G^G[ACGT]CC
SexAI 66 ACCAGGT A^CC[AT]GGT
SspBI 741 TGTACA T^GTACA
StyI 291 CCTTGG C^C[AT][AT]GG
TaqI 188 TCGA T^CGA
TfiI 251 GAATC G^A[AT]TC
TseI 444 GCTGC G^C[AT]GC
Tsp45I 154 GTGAC ^GT[GC]AC
TthHB8I 188 TCGA T^CGA
Van91I 351 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 36 TCTAGA T^CTAGA
XcmI 237 CCACAGCATCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 618 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 348 CCGC CCGC(-3/-1)
523 CCGC
BbsI 6 GAAGAC GAAGAC(2/6)
199 GAAGAC
BpuAI 6 GAAGAC GAAGAC(2/6)
199 GAAGAC
BsaAI 280 TACGTG [CT]AC^GT[GA]
830 CACGTG
BsaJI 291 CCTTGG C^C[ACGT][ACGT]GG
683 CCCAGG
BsmFI 372 GGGAC GGGAC(10/14)
413 GTCCC
BssKI 67 CCAGG ^CC[ACGT]GG
684 CCAGG
BstF5I 561 GGATG GGATG(2/0)
756 GGATG
BstNI 67 CCAGG CC^[AT]GG
684 CCAGG
DdeI 845 CTGAG C^T[ACGT]AG
858 CTTAG
DpnII 319 GATC ^GATC
619 GATC
EcoRII 67 CCAGG ^CC[AT]GG
684 CCAGG
FokI 561 GGATG GGATG(9/13)
756 GGATG
HinfI 9 GACTC G^A[ACGT]TC
251 GAATC
MaeIII 63 GTTAC ^GT[ACGT]AC
154 GTGAC
MboI 319 GATC ^GATC
619 GATC
MslI 322 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
681 CACCCAGGTG
MvaI 67 CCAGG CC^[AT]GG
684 CCAGG
NdeII 319 GATC ^GATC
619 GATC
NlaIII 486 CATG CATG^
655 CATG
Sau3AI 319 GATC ^GATC
619 GATC
ScrFI 67 CCAGG CC^[ACGT]GG
684 CCAGG
SfcI 136 CTACAG C^T[GA][CT]AG
445 CTGCAG
StuI 378 AGGCCT AGG^CCT
670 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BfaI BsiYI BslI BsrI
HaeIII MaeI MaeII MboII MnlI
MseI SfaNI SmlI Tru9I Tsp509I
TspRI