Restriction map for
pF1KB3632
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII AccI AccII AclI AcsI
AflII AgeI AhdI Alw44I ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
AvrII BanII BbvCI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV FseI
FspI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tsp509I Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 300 GGTACC G^GTACC
AccIII 33 TCCGGA T^CCGGA
AcyI 1051 GGCGCC G[GA]^CG[CT]C
Aor51HI 533 AGCGCT AGC^GCT
Asp718I 300 GGTACC G^GTACC
BalI 719 TGGCCA TGG^CCA
BamHI 366 GGATCC G^GATCC
BbeI 1051 GGCGCC GGCGC^C
BbrPI 171 CACGTG CAC^GTG
BbsI 888 GAAGAC GAAGAC(2/6)
BclI 773 TGATCA T^GATCA
BglI 110 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 805 CTGGAG CTGGAG(16/14)
BpuAI 888 GAAGAC GAAGAC(2/6)
BsaAI 171 CACGTG [CT]AC^GT[GA]
BsaHI 1051 GGCGCC G[GA]^CG[CT]C
BsaI 549 GAGACC GGTCTC(1/5)
BsaWI 33 TCCGGA [AT]^CCGG[AT]
BseAI 33 TCCGGA T^CCGGA
BsmAI 549 GAGAC GTCTC(1/5)
BspEI 33 TCCGGA T^CCGGA
BspLU11I 1044 ACATGT A^CATGT
BstAPI 210 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 291 GGTGACC G^GT[ACGT]ACC
BstPI 291 GGTGACC G^GT[ACGT]ACC
BstYI 366 GGATCC [GA]^GATC[CT]
EaeI 719 TGGCCA [CT]^GGCC[GA]
Eco47III 533 AGCGCT AGC^GCT
EcoNI 304 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 291 GGTGACC G^GT[ACGT]ACC
EcoT22I 932 ATGCAT ATGCA^T
FauI 744 GCGGG CCCGC(4/6)
FbaI 773 TGATCA T^GATCA
HgaI 219 GCGTC GACGC(5/10)
Hin1I 1051 GGCGCC G[GA]^CG[CT]C
KasI 1051 GGCGCC G^GCGCC
KpnI 300 GGTACC GGTAC^C
MflI 366 GGATCC [GA]^GATC[CT]
MluNI 719 TGGCCA TGG^CCA
MroI 33 TCCGGA T^CCGGA
MscI 719 TGGCCA TGG^CCA
MseI 334 TTAA T^TAA
MslI 285 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1051 GGCGCC GG^CGCC
NsiI 932 ATGCAT ATGCA^T
PmaCI 171 CACGTG CAC^GTG
PmlI 171 CACGTG CAC^GTG
PstI 208 CTGCAG CTGCA^G
SfcI 208 CTGCAG C^T[GA][CT]AG
SfoI 1051 GGCGCC GGC^GCC
SmlI 510 CTCAAG C^T[CT][GA]AG
SphI 104 GCATGC GCATG^C
TaqI 1092 TCGA T^CGA
Tru9I 334 TTAA T^TAA
TthHB8I 1092 TCGA T^CGA
XhoII 366 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 448 CCGC CCGC(-3/-1)
744 GCGG
AfaI 268 GTAC GT^AC
301 GTAC
AlwI 367 GATCC GGATC(4/5)
366 GGATC
AvaI 146 CCCGGG C^[CT]CG[GA]G
872 CCCGGG
BsoBI 146 CCCGGG C^[CT]CG[GA]G
872 CCCGGG
EarI 682 CTCTTC CTCTTC(1/4)
340 GAAGAG
HaeII 533 AGCGCT [GA]GCGC^[CT]
1051 GGCGCC
HinfI 204 GACTC G^A[ACGT]TC
652 GAGTC
Ksp632I 682 CTCTTC CTCTTC(1/4)
340 GAAGAG
NspI 104 GCATGC [GA]CATG^[CT]
1044 ACATGT
PflMI 273 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
530 CCAAGCGCTGG
PleI 204 GACTC GAGTC(4/5)
652 GAGTC
PvuII 568 CAGCTG CAG^CTG
643 CAGCTG
RsaI 268 GTAC GT^AC
301 GTAC
SapI 340 GAAGAGC GCTCTTC(1/4)
681 GCTCTTC
SmaI 146 CCCGGG CCC^GGG
872 CCCGGG
StuI 188 AGGCCT AGG^CCT
753 AGGCCT
Van91I 273 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
530 CCAAGCGCTGG
XmaCI 146 CCCGGG C^CCGGG
872 CCCGGG
XmaI 146 CCCGGG C^CCGGG
872 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AflIII AluI AlwNI AocI AvaII
BanI BbvI BcnI BfaI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PpuMI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
TspRI